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Owing to the inaccessibility of substrate, the physiological function of aleurone beta-amylase is unknown. Some lipases reported possess both lipase and phospholipase activities.

因此推测玉米的beta淀粉酶可能和大豆beta淀粉酶同样具有抑制脂解酶的能力,其存在可以部分抑制脂解酶活性。

Cloning of the Schwanniomyces occidentalis α-amylase and high expression in S.cerevisiae.The E.coli / yeast shuttle plasmid YCEpl partial library of Schwanniomyces occidentalis DNA was constructed and α-amylase gene fragments were screened in Saccharomyces cerevisiae by amylolytic activity.Several transformants with amylolysis were obtained and one of the fusion plasmids has about 5.0 kb inserted DNA fragment.It contains the upstream and downstream sequences of α-amylase gene from S.occidentalis .

以E.coli/yeast穿梭质粒YCEp1为载体构建西方许旺酵母部分基因组文库,在大肠杆菌中扩增后提取混合质粒DNA,经电转化非缺陷标志酒精酵母 AS.2.1364,在YPDS平板(含1%葡萄糖和1%可溶性淀粉)上用淀粉水解活力筛选含水解淀粉的阳性转化子,从阳性转化子中分离重组质粒证实含5.0kb的插入片段,用α-淀粉酶基因两端序列设计的引物PCR扩增及扩增片段序列分析证实该片段中含有α-淀粉酶全部编码序列。

Though monitoring of the bacteriolysis of lysozyme to M. Lysodeikeicus and the hydrolysis of soluble starch catalyzed by α-amylase, lysozyme and α-amylase in human saliva were determined.

通过溶菌酶对溶壁微球菌的溶菌作用及α-淀粉酶对淀粉的水解作用,成功地测定了人体唾液中的溶菌酶和α-淀粉酶。

A form of amylase in the saliva of human beings and some animals that catalyzes the hydrolysis of starch into maltose and dextrin.

唾液淀粉酶在人类或某些动物的唾液中的一种淀粉酶,能把水解淀粉催化成麦芽糖和糊精

The effect of electric field strength on the secondary structure of α-amylase was studied by circular dichroism after treating it with different strength of electric field.

α-淀粉酶被不同强度的电场作用,用圆二色光谱研究不同强度的电场对α-淀粉酶二级结构的影响。

A form of amylase in the saliva of human beings and some animals that catalyzes the hydrolysis of starch into maltose and ''.

唾液淀粉酶在人类或某些动物的唾液中的一种淀粉酶,能把水解淀粉催化成麦芽糖和糊精

The inhibitory effect of NaCl on the halophytic seeds decreased with the delay of treatment time, but that on nonhalophytic seeds incresed up to the 8 th day, after then did not incre...

另外 ,在 0 。6MPaNaCl下,随处理时间延长,盐地碱蓬和琐琐种子的α淀粉酶活性不断提高,SOD活性基本不变,但小麦和大麦种子的α淀粉酶活性在处理 8d后接近最大值,SOD活性显著下降。

During hydrolysis, when both the mesothermal-α-amylase and fungal-α-amylase were used, the transformation ratio of the buckwheat starch hydrolysis surpassed 99.5%. It also could get a higher ratio when two kinds of enzymes mixed with different proportion.

在荞麦淀粉的水解过程中,中温α-淀粉酶与真菌α-淀粉酶对荞麦淀粉的水解转化率均超过99.5%,两种酶按不同比例混合使用对淀粉的水解转化率更高。

Its activity was specific and was affected by pH, reaching the peak in weak acidic condition. It obviously inhibits amylases from D. melanogaster, Solenopsis invicta, Odontotermes formosanus, Periplaneta Americana Linnaeus, and Alphitobius sp. In addiction, the study points out Phaseolus vulgaris, Phaseolus coccineus L. and Glycine max, which have significant inhibition toward pancreatic amylase.

经测试发现此抑制剂在85℃时仍具备抑制果蝇淀粉酶之活性,为一热安定性蛋白质,其抑制作用受pH 值影响很大,在偏酸性环境下的效果最好,且其抑制作用具特性,可明显抑制果蝇、入侵红火蚁、白蚁、蟑螂及面包虫等昆虫的淀粉酶活性。

The protease activity in foregut was higher than that of midgut in an acidic condition at pH 5.0-5.8, while in an alkalescence condition at pH 7.0-8.6, the protease activity was higher in midgut than that in foregut. The lipase activity of foregut was high and stable in two pH ranges: 4.2-5.0 and 6.2-7.0, and that of midgut was high at pH 3.8, while the lipase lost enzyme activity at pH over 9.0. The amylase activity was high and stable at pH 6.6-7.4 in both foregut and midgut. The cellulase activity of foregut and midgut was high and stable at pH 6.2-7.4 and 5.4-7.0 respectively.

刺参前肠蛋白酶活力在酸性环境下较中肠高,且在反应pH为5.0-5.8之间酶活力相对稳定,而中肠蛋白酶活力在碱性环境下较前肠高,且在反应pH为7.0-8.6之间酶活力相对稳定;刺参前肠脂肪酶活力随着pH的升高出现两个相对稳定的峰值,分别为4.2-5.0和6.2-7.0区间,中肠脂肪酶活力在pH为3.8时达到最大值,而在pH超过9.0明显失活;刺参前肠和中肠淀粉酶活力在pH变化时表现出相似的变化趋势,在反应pH为6.6-7.4之间酶活力较高且相对稳定;刺参前肠和中肠中纤维素酶活力在pH变化时反应不一致,前肠淀粉酶在反应pH为6.2-7.4之间酶活力较高且相对稳定,中肠纤维素酶活力在反应pH为5.4-7.0之间酶活力较高且相对稳定。

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The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作:对伸臂位于顶部的单层框架—筒体模型进行分析,提出了等效转动约束的概念和转动约束刚度的表达式。

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Its advantage is that it can be used in smaller units.

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