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By comparing the activity of theα-amylase and the soluble acid invertase in the kernel and the pedicel, the trend of theα-amylase activity in the kernel and the pedicel showed a peak, theα-amylase activity was high in the early stage, it could promoted amylolysis, supply the nutrition for the kernel development; theα-amylase activity was low in the rapid filling stage, it was in favorable to the starch accumulation.

通过比较籽粒和小穗柄中α-淀粉酶和可溶性酸性蔗糖转化酶的活性表明,籽粒和小穗柄中α-淀粉酶活性均呈单峰变化趋势,籽粒发育早期α-淀粉酶活性较高,可以促使淀粉转化,为籽粒的早期的发育提供营养物质;籽粒发育中期为快速灌浆期,淀粉酶活性较低,利于淀粉的积累。

BFEPP has a designed productive capacity of 30,000 standard tons each year, a production level well ahead of its domestic counterparts. Now BFEPP has 11 lines of products, including amylase765, basified protease2709, neutral protease1398, acid protease, pullulanase, cellulase, pectinase, carbohydrase,β-glucans, biological pesticide, intermediate-temperature amylase element and high-temperature amylase element.

该厂是北京市认定的高新技术企业,年设计生产能力为30000标吨,现在形成12大系列产品,其中包括:淀粉酶765、碱性蛋白酶2709、中性蛋白酶1398、酸性蛋白酶、异淀粉酶、纤维素酶、果胶酶、糖化酶、β-葡聚糖酶、高温淀粉酶、淀粉糖和。

A conclusion was reached that as all various factors jointly play their roles, the dosages of Daqu and proteinase had prominent impact on the formation of fusel oil, while adding saccharifying enzyme and dry yeast had less great effect on the generation of fusel oil, but adding the amylase could barely influence the formation of fusel oil.

得出在各因素共同作用的情况下,大曲用量和蛋白酶用量显著影响杂醇油的生成量,而干酵母和糖化酶的添加也可以影响杂醇油的生成,但效果不显著,淀粉酶的添加则几乎对杂醇油的生成没有影响。

Significantly genotypic differences and great variations were detected in grain β-amylase activity,β-glucan and protein fraction contents. The largest variation and highest average value of β-amylase activity were found in Hangzhou 2006-2007, while the trend of coefficient of variation was opposite to the mean content of β-glucan over different environments. Hordein content had the highest correlation coefficient with β-amylase activity among four protein fractions.

研究结果表明:籽粒β-淀粉酶活性、β-葡聚糖和蛋白组分含量均存在显著的基因型差异;杭州2006-2007年度种植条件下的β-淀粉酶活性均值和变异幅度最高,且β-淀粉酶活性和β-葡聚糖含量的变异系数和变化趋势恰好相反;4种蛋白质组分中,醇溶蛋白含量与β-淀粉酶活性呈显著正相关。

And found root exudates have the promotable effect on the activity of IAAoxidase of cucumber and squash,.

在对其生理生化特性的研究中发现,根系分泌物显著的促进黄瓜和南瓜体内吲哚乙酸氧化酶的活性,从而降低其体内吲哚乙酸的水平,抑制其生长发育;根系分泌物还显著抑制受体淀粉酶的活性,这种抑制性随浓度升高而增加,通过影响黄瓜和南瓜体内的能量转化来影响其正常生长。

Four goats (42.4±3.5 kg) surgically fitted with cannulas in common bile duct and abomasal were used in 4×4 Latin square design and randomly fed diets contained 20%, 30%, 40% or 50% of starch to determine the effects of rumen escape starch, by altering dietary starch concentration, onα-amylase secretion in goats. Increasing rumen escape starch results in a quadratic increase in pancreaticα-amylase secretion.

活体分泌试验表明,日喂3次情况下,胰胆汁分泌量和α-淀粉酶分泌活性呈非匀速分泌,在饲喂前后1h内最高(P.05);胰腺α-淀粉酶总分泌活性随随过瘤胃淀粉数量的提高呈二次曲线变化(P.05),过瘤胃淀粉为102g/d时胰腺α-淀粉酶总分泌量达到最大。

To examine the hypothesis, we test whether maize beta-amylase can serve as a lipase inhibitor.In order to conduct the interaction assay of beta-amylase and lipase, we adopted a preparative electrophoresis method with a Prep Cell to purify beta-amylase proteins from germinating maize endosperms and soybean cotyledons. We pre-mixed tested proteins with lipid substrate prior to the addition of lipase preparation and assayed the lipid hydrolytic activity.

本实验利用Prep Cell纯化玉米萌芽谷粒和大豆子叶中的beta-淀粉酶,分别将两种不同物种的beta-淀粉酶与胰脂解酶预先反应后,再加入脂解酶反应液中观察活性的变化,结果发现脂解酶的活性未如预期,没有抑制的效果产生,将甘薯beta-淀粉酶以同样方法与胰脂解酶作用,结果同上述两种beta淀粉酶依旧没有抑制的现象。

The results of its fluorescence probe showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L,there existed some stable hydrophobic regions,which could interact with a hydrophobic reagent 8-anilino-1-naphthalene sulfonic acid,in the partially folded intermediate of Bacillus amyloliquefaciensα-amylase;with the denaturation concentration increasing,the stable hydrophobic regions disappered.the results of fluorescence quenching using acrylamide and potassium iodide as quenchers showed that using acrylamide as quenchers,with the protein denaturation extent increasing,the number of Trp that can be quenched increased untill all the Trp residues were quenched;Using potassium iodide as quenchers,with the maximum number(8) of tryptophan residues in a partially folded intermediate Bacillus amyloliquefaciensα-amylase molecule could be quenched by potassium iodide;with the denaturation concentration increasing,the number of Trp that can be quenched decreased to 5.the results of their protein electrophoreses and SEC showed that no aggregate or aggregate precipitation of Bacillus amyloliquefaciensα-amylase formed during the whole unfolding/refolding procedure of Bacillus amyloliquefaciensα-amylase induced by guanidine hydrochloride or urea.

ANS外源荧光探针结果表明:盐酸胍诱导的芽孢杆菌α-淀粉酶分子去折叠过程中存在着能够与探针分子1-苯胺基-8-萘磺酸结合的稳定的疏水区域;而随着芽孢杆菌α-淀粉酶分子在盐酸胍溶液中变性程度的加深,这一疏水区域逐步被瓦解。丙烯酰胺和碘化钾猝灭结果表明:在盐酸胍溶液中,随着芽孢杆菌α-淀粉酶分子变性程度的进一步加深,其分子内能够被丙烯酰胺接近的色氨酸残基逐渐增多,直至全部被猝灭。但位于芽孢杆菌α-淀粉酶分子表面的能够被碘化钾猝灭的色氨酸残基,在中间态芽孢杆菌α-淀粉酶分子中数目达到最大的8个,而随着其分子变性程度的进一步加深,反而减少至5个。

In this thesis, three kinds of starch enzyme s were taken to hydrolyze potato flour to make fat mimics of carbohydrate style under control.

本文采用不同的淀粉酶控制水解马铃薯淀粉制备低DE麦芽糊精作为脂肪模拟品,研究不同淀粉酶作用产物的不同性质,确定了碳水化合物型脂肪模拟品生产用酶为耐高温α-淀粉酶,最佳工艺条件为:酶添加量为0。

Potato flour, soybean powder, FeSO_4 were screened out as the most suitable carbon source, nitrogen source and inorganic salt source, respectively. Based on these, screening methodology Plackett-Burman design was used to evaluate the effects of twelve factors related to amylase production and three statistically significant factors soybean powder, temperature, bran were selected.

酶对底物的作用方式表现出多样性,以表面扩散侵蚀的方式作用于马铃薯和木薯生淀粉,以表面扩散侵蚀和形成大侵蚀孔洞的方式同时作用于红薯淀粉。4、利用响应面法对菌株AF-1固态发酵产生淀粉酶的条件进行了优化。

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