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In this project, in order to develop a designable and controllable mixed microbial culture with intelligent signals, we chose the alkalophilic mixed culture as model system for production of multienzymes consisting of alkaline cellulase and alkaline amylase, and the basic characteristics of the mixed culture was examined.

本项目选择了能够生产碱性淀粉酶和碱性纤维素酶的嗜碱微生物复合体系来生产复合酶,重点研究嗜碱菌复合培养过程的特点及基于荧光蛋白基因快速定量解析复合培养体系的组成菌种及其相互作用的方法论基础。

The result shows that taking the liquefaction technique under the condition that the starch raw material is out of the process of making koji can save 40% of starch raw material and the overplus can be used to produce the modified starch.

结果表明,采用淀粉酶液化工艺后,淀粉质原料不参与制曲,可节约这部分原料的40%,节约下来的原料可制成变性淀粉,提高酱油的无盐固形物含量,增加酱油的粘稠度。

When the concentration ratio of skatole to enzymes is 5/1, the inhibition ratios of skatole on those three enzymes are 32.4 % 28.7% and 26.6%.

当向三种消化酶中加入不同浓度的粪臭素时,随着粪臭素浓度的增加,α-淀粉酶在265 nm附近、胃蛋白酶在275 nm附近及胰蛋白酶在270 nm附近的紫外吸收峰均有一定程度的升高。

Ap with hyperlipaemia model was prepared by both triton wr1339 and cerulein simultaneously. human albumin was used to treat the ap accompanied with hyperlipaemia. in each group, the histological score, volume of ascites, ratio of pancreatic wet/dry weigh, serum amylase and pancreatic acinar cell apoptosis in each group were compared. the level of protein kinase c membrane translocation in pancreatic tissue was detected by western blot.

分别用triton wr1339、cerulein制作大鼠高脂血症和急性胰腺炎(acute pancreatitis,ap)模型,同时应用两者制作伴有高脂血症的ap模型,应用白蛋白治疗伴有高脂血症的ap,比较各组胰腺病理损害评分、腹水量、胰腺湿/干比、血清淀粉酶和胰腺组织的凋亡;western blot检测胰腺组织pkc的膜转位。

Methods Hyperlipaemia and AP model were established with Triton WR1339 and Cerulein respectively. AP with hyperlipaemia model was prepared by both Triton WR1339 and cerulein simultaneously. Human albumin was used to treat the AP accompanied with hyperlipaemia. In each group, the histological score, volume of ascites, ratio of pancreatic wet/dry weigh, serum amylase and pancreatic acinar cell apoptosis in each group were compared. The level of protein kinase C membrane translocation in pancreatic tissue was detected by Western blot.

分别用Triton WR1339、Cerulein制作大鼠高脂血症和急性胰腺炎(acute pancreatitis,AP)模型,同时应用两者制作伴有高脂血症的AP模型,应用白蛋白治疗伴有高脂血症的AP,比较各组胰腺病理损害评分、腹水量、胰腺湿/干比、血清淀粉酶和胰腺组织的凋亡;Western blot检测胰腺组织PKC的膜转位。

At higher dietary protein levels, the activity of pepsin and tryptase increase significantly, while the ratio of the activity of amylase to tryptase decrease. No correlation between growth and activity of digestive enzymes is found in present work.

随着饲料中蛋白水平的上升,3种消化酶的比活性也呈逐渐上升的趋势,而淀粉酶与类胰蛋白酶比活性的比值逐渐下降,表明罗氏沼虾消化酶活性对饲料中蛋白含量有适应性反应。

Saccharifying enzyme and β-amylase were added in the preparation of mellow sugar solution to increase the sugar content and the solution quality.

糖化发酵阶段使用1 %的混合酒曲和15 u/g的酸性蛋白酶及0.1 %的黄酒活性酵母;制备醇香糖液时添加0.1 %的糖化酶和β-淀粉酶,提高含糖量和品质。

Gelatinization degree determination and texture profile analysiswere used in the study on the effect of amylases on retrogradation of instant rice .

通过测定不同样品的糊化度和质构特性来研究几种淀粉酶对即食米饭老化的影响,希望能够较好的解决即食米饭的老化问题。

Study on effect factors of liquidation of potato flour;2. According to an enzymatic process for the manufacturing of high maltose syrup through orthogonal test, the factors affecting the liquidation process of potato starch were studied deeply in this paper with the raw material of potato starch.

以马铃薯淀粉为原料,利用酶制剂制备高麦芽糖浆,采用正交实验法,对马铃薯淀粉液化过程的影响因素进行了详细研究,得到液化工艺的最佳条件为:淀粉浆浓度40%、耐高温α-淀粉酶用量106U/g淀粉、液化温度94℃、液化时间10min,所得液化液的DE值为9。

Grain β-amylase activity and protein content were significantly higher in the treatments where all top-dressed N fertilizer was applied at booting stage or equally applied at two-leaf stage and booting stage than in the treatment where all N fertilizer was applied at two-leaf age stage.

籽粒β-淀粉酶活性和蛋白质含量,追肥孕穗期一次性施和二叶期与孕穗期各施50%的处理显著高于二叶期一次施的处理,且以孕穗期一次施的处理为最高。

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