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The results showed that the optimal pH were 2.0 and 6.5~7.5 for gastric protease and amylase,10.5 and 6.5 for intestinal protease and amylase and 7.5 and 8.0 for intestinal trypsin and chymotrypsin,respectively.

结果表明:胃蛋白酶和淀粉酶的最适pH分别为2.0和6.5~7.5,肠蛋白酶和淀粉酶的最适pH分别为10.5和6.5,肠胰蛋白酶、糜蛋白酶的最适pH分别为8.5和8.0。

The results show that the inhibition effect of phytic acid is obvious,and it is an ideal improver for quality of germinant wheat.

通过测定不同植酸添加量下的α-淀粉酶活性及降落值,表明植酸对α-淀粉酶的抑制效果十分明显,是一种理想的芽麦品质改良剂。

GIB852 could efficaciously inhibit human pancreatic α-amylase and α-maltase glucoamylase. The inhibition of maltase is stronger than the same dose of the commercial α-glucosidase inhibitor miglitol 28.7 times, while the inhibition of α-amylase is weak.

GIB852对人类胰腺α-淀粉酶和α-麦芽糖酶都有明显的抑制作用,其对麦芽糖酶的抑制性比同剂量的市售双糖酶抑制剂米格列醇强28.7倍,而对α-淀粉酶的抑制性较弱。

When chinese wampee seeds were naturally desiccated, the content of soluble sugar in seeds increased, and the content of starch in seeds decreased. In the meantime, the activities of phosphorylase and isocitrate lyase in seeds, and of α-and β-amylase in embryonic axes enhanced initially, and then fell.

自然脱水时,黄皮种子中可溶性糖的含量增加,淀粉含量下降;磷酸化酶,异柠檬酸裂解酶以及胚轴中α-和β-淀粉酶的活性先增加然后下降:子叶中α-和β-淀粉酶的活性呈下降趋势。

The study suggested that the optimal method not only enhanced the affinity and the maximum reaction velocity of α-amylase with its substrate, but also improved the accuracy and the sensitivity on the activity of α-amylase from M. separata larvae in assays, whereas it was not the optimal method for determining the activity of α-amylase from T. molitor, C. pipiens pollens and M. domestica larvae.

结果说明,在该优化体系下,粘虫α-淀粉酶与底物的亲和力增强,最大反应速度增大,测定酶活性的准确性和灵敏度显著提高;同时该优化体系也可作为测定德国小蠊α-淀粉酶活性的优化方法,但不适合作为黄粉虫、淡色库蚊和家蝇α-淀粉酶的最优化测定方法。

An amylase or a mixture of amylases that converts starch to maltose, found in certain germinating grains such as malt.

淀粉酶一种把淀粉或麦芽糖的淀粉酶或淀粉酶的混合物,可在某些发芽的谷物比如麦芽中找到

An amylase or a mixture of amylases that converts starch to malt ose,found in certain germinating grains such as malt .

淀粉酶一种把淀粉或麦芽糖的淀粉酶或淀粉酶的混合物,可在某些发芽的谷物比如麦芽中找到

Shaking culture with the liquid containing 1.5×10^6 connidia per millilitre for 3.5 d at the temperature of (25±1)℃ and 120r/min resulted in max mycelial of 5.1mg/mL and amylase yield of 272.42U/mg. An initial range of pH 4~6 in the liquid culture was the most favorable to amylase production with the yields of 1377.20~1528.99 U/mg. The activity of E.

在孢子浓度为1.5×10^6个/mL的PD培养液中恒温(25±1)℃振荡(120r/min)培养3.5d,菌丝生物量最大达5.1mg/mL,产淀粉酶量达272.42U/mg;培养液初始pH4~6最有利淀粉酶的产生,产酶量为1377.20~1528.99U/mg菌丝。

The results of its intrinsic fluorescence spectroscopy and fluorescence phase diagram showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed a partially folded intermediate of Bacillus amyloliquefaciens a-amylase during its unfolding procedure, which followed a three-state model; the result of its fluorescence probe showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed some stable hydrophobic regions, which could interact with a hydrophobic reagent 8-anilino-1-naphthalene sulfonic acid, in the partially folded intermediate of Bacillus amyloliquefaciens a-amylase; and the results of fluorescence quenching using acrylamide and potassium iodide as quenchers showed the distribution of Trp residues in Bacillus amyloliquefaciens a-amylase in different denaturation solution, with the maximum number (8) of tryptophan residues in a partially folded intermediate Bacillus amyloliquefaciens a-amylase molecule could be quenched by potassium iodide; and the results of their protein electrophoresis and SEC showed that no aggregate or aggregate precipitation of Bacillus amyloliquefaciens a-amylase formed during the whole unfolding procedure of Bacillus amyloliquefaciens a-amylase induced by guanidine hydrochloride.

内源荧光光谱和荧光相图结果表明,当变性液中盐酸胍浓度约为1.0 mol/L时,芽孢杆菌a-淀粉酶的去折叠过程中出现一个部分折叠中间体,其去折叠过程符合&三态模型&;荧光探针结果表明,在溶液中盐酸胍浓度约为1.0 mol/L时,中间态芽孢杆菌a-淀粉酶分子中存在着能够与探针分子1-苯胺基-8-萘磺酸结合的稳定的疏水区域;荧光猝灭研究给出了不同程度变性的淀粉液化芽孢杆菌a-淀粉酶中的Trp的分布情况,结果表明中间态芽孢杆菌a-淀粉酶分子中能够被碘化钾猝灭的位于分子表面的色氨酸残基数目达到最大的8个;蛋白电泳和体积排阻色谱结果表明,在盐酸胍诱导的芽孢杆菌a-淀粉酶分子的整个去折叠过程中,不会以共价键或非共价键形式形成芽孢杆菌a-淀粉酶分子之间的集聚体或集聚体沉淀。

Taking the wild snail Su bag as the raw material to extract the snail enzyme product,and determining the activity of cellulose enzyme and starch enzyme .

以野生蜗牛嗉囊为原料提取蜗牛酶产品,检测所含纤维素酶、淀粉酶的活性,对其蜗牛酶产品进一步测试,结果表明新疆蜗牛酶中的纤维素酶活力为450U/g 淀粉酶为405U/g。

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