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The image analyzing results showed cNHX1 protein was not located in mitochondria but in vacuole membrane.

图象分析结果表明cNHX1蛋白没有定位于线粒体,而是位于液泡膜上。

Membrane that surrounds a vacuole of a plant ''.

液泡膜包围植物''。

In cells which began degeneration and thus stopped secretion, enzyme reaction product was localized mainly on the vacuole membrane.

分泌活动停止后处于解体状态的细胞内,反应产物主要定位于液泡膜上。

NaCl and iso-osmotic PEG had inhibitory effect on the germination of two halophytic seeds, and the inhibitory level of PEG was greater than that of iso-osmotic NaCl. Inhibitory effects of NaCl and water stress mainly showed lowering the seed germination rate, delaying initial time of seed germination and extending the time of seed germination.

本研究从生理和分子角度对其耐盐性进行了初步的探讨,开展这两种盐生植物耐盐相关基因的克隆及盐爪爪液泡膜上多个耐盐相关基因的遗传工程,为作物和林木的遗传改良奠定坚实的理论和实验基础。

The immature stage pointed to the development of SE before vacuole membrane was ruptured.In this stage,the cell radial expanding,cell wall incrassation,sieve element plastid and P-protein producing of SE occurred.

未成熟期是指筛管液泡膜破裂之前筛管的发育阶段,在这一时期内出现了细胞的径向扩展、壁的增厚以及筛管质体和P-蛋白质的产生。

Specific primers were designed according to gene NHX1 of Limonium gmelinii published on NCBI. Then, the gene was cloned by means of RT-PCR. Eukaryotic expression vector pCAM-BIA1301-NHX1 had been constructed, then transformed into tomato by Agrobacterium-mediated transformation method. Transgenic tomatos were obtained.

根据NCBI公布的大叶补血草NHX1基因序列设计特异性引物,经RT-PCR方法克隆得到大叶补血草液泡膜Na/H逆向转运蛋白基因NHX1,构建植物融合表达载体pCAMBIA1301-NHX1,重组质粒通过农杆菌介导转化番茄,经PCR和RT-PCR鉴定,获得转基因番茄植株。

The following results have been obtained: 1 Expression of SbNHX1 in E. coli enhanced Na+ and K+ tolerances, but not to others; 2 The sequence from 1353-1514bp in SbNHX1 gene played a role for SbNHX1 to Na+ and K+ tolerances; 3 The Na+/H+ antiporter of Salicornia Bigelovii Torr. catalysed Na+/H+ or K+/H+ exchange with equal affinity, which indicates that the SbNHX1 is more likely to locate on member of vacuolar, and responds to transport Na+ into the vacuole from cytoplasm.

证明了SbNHX1能抵抗Na+和K+的胁迫,但对其它盐离子没有抗性;2)证明了北美海蓬子Na+/H+逆向转运蛋白基因(SbNHX1)C末端从基因的C末端1353bp至1514bp的序列在耐盐中起一定的作用;3)证明了北美海蓬子Na+/H+逆向转运蛋白(SbNHX1)对Na+和K+交换的等亲和性,对于这一点,说明北美海蓬子Na+/H+逆向转运蛋白与定位于液泡膜上的Na+/H+逆向转运蛋白的功能相似。

Autophagy, appears to involve an active participation of the vacuolar membranes.

自体吞噬似乎与液泡膜的积极参加有关。

Two types of pyrophosphatase are present in plant tissues: soluble one in cytoplasm and insoluble one on the vacuolar membrane.

植物组织中有两种类型的焦磷酸酶,一类是细胞质中可溶性的无机焦磷酸酶,另一类是与细胞液泡膜结合的不可溶性焦磷酸酶(vacuolar PPase,V-PPase)。

These results suggest that the products of the novel gene,mNHX1,on the vacuolar membrane,functions as a Na~/H~ antiporter and may be one of the most important factors determining salt tolerance in mulberry trees .

根据已报道的At-NHX1同源基因的功能推断,mNHX1所编码的细胞液泡膜上Na+/H+逆向运转蛋白是桑树抗盐性能的一个极其重要的因素。

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