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Moreover, the heterozygote is refrigerated at low temperature or frozenly reserved in liquid nitrogen for a long time, and after resuscitation, the heterozygote can be directly used in the repair of tissues or organs so as to effectively avoid the necrosis of tissues due to untimely use after assembling.

然后在低温下泠藏或在液氮中长期冻存,复苏后可直接用于组织或器官的修复,有效避免了细胞在组装后若不及时使用就会出现的坏死现象。

Methods By intratracheally instillation, two strains of mice (C57BL/6 and C3H/He mice) were exposed to PM2.5 once time per day, for continuously two days.

采用气管滴注的染毒方法,两种品系的小鼠(C57BL/6小鼠和C3H/He小鼠)均予PM2.5染毒,连续染毒2d,最后一次染毒24h后,处死小鼠,取肺组织,并且立即置于液氮中保存。

All of them were labeled with [32P] orthophosphate (2.03×10^5 Bq/mL) for 2 h and then stimulated with CCK8 l0^(-7 mol/L and DMEM respectively for different time. Reactions were terminated by freezing neurons in liquid nitrogen. The 32P-labeled lysates isoelectrically focused on IPG Drystrip pH 4~7 linear gels and subsequently separated by second-dimensional SDS-PAGE.

培养乳小鼠神经元,随机分为CCK8刺激组和对照组,32P-NaH2PO4标记后分别给予CCK810^(-7 mol/L和等量无磷培养基,作用不同时间后液氮终止反应,经双向电泳、放射自显影,获得磷酸化蛋白质的电泳图谱。

After freezing and thawing,the ability for itaconic acid formation of Asp.terreus AS 3.2811,AS 3.2822,...

所测定的液氮保存8株菌种的生理活性,除两株糖化酶活力稍有降低外,其它菌株没有明显的变化。

It was shown in our experiment that boiling heat transfer coefficient of forced liquid nitrogen flow in lunate channel is 3~5 times larger than that in smooth tube.

摘 要:对液氮在0.5~1.5mm狭缝通道内受迫流动沸腾换热的情况进行了研究。

In this paper lunate channel is adopted to study forced flow boiling heat transfer and its pressure drop in detail.

因此本文采用这种独特的狭缝通道结构形式——弦月形狭缝通道,实验和理论研究了液氮在0.5~1.5mm狭缝通道内受迫流动沸腾时的传热特性和流动特性。

It was shown in our experiment that boiling heat transfer coefficient of forced liquid nitrogen flow in lunate channel is 3~5 times larger than that in smooth tube.

液氮在0.5~1.5 mm狭缝通道内受迫流动沸腾换热的情况进行了研究。

In one case of which phrenic nerve was transferred to the musculocutaneous nerve (the nerve graft 8 cm), the biceps showed some nerves regeneration shown in the EMG 15 months postoperatively.

方法采用特制保护液浸泡、液氮冷冻及断面修整、粘合与固定等轴索修复技术,对12例周围神经(13根神经)损伤的患者进行轴索水平的修复,术后平均随访9.7个月。

Methods The active homograft bioprosthetic pediculated conduit preserved in liquid nitrogen were selected as experimental material and decellularized by sodium dodecyl sulplate containing low osmotic Tris buffer.

方法选取经液氮保存、具有活性的同种生物带瓣管道作为实验材料,用含十二烷基硫酸钠的Tris低渗缓冲液脱去同种带瓣管道存在的细胞;固定剂固定后,分别行HE染色、胶原纤维和弹力纤维染色的光镜和扫描电镜观察、摄片。

The result indicated that: the amount of the excess HF formed direct ratio to the yield of phosphorus pentafluoride and the optimum amount of the excess HF used is about 35%; the trickling rate of oleum shouldnt make the temperature of hexafluorophosphoric acid exceed 25 ℃ and the amount of oleum is 110% to 125% that of stoichiometry so that the content of phosphorus pentafluoride can be achieved 90%; the time and temperature of heating hexafluorophosphoric acid influenced both the yield and the content of phosphorus pentafluoride, and the optimum time should be about 30 minutes and the proper temperature was 148 to 153℃; when alcohol used asrefrigerant, the content of phosphorus pentafluoride was 94.03%, and the content can be up to 97.38%, provided that using liquid nitrogen as refrigerant.

通过实验发现,氟化氢用量与五氟化磷的产率成正比,最佳过量百分率宜在35%左右;发烟硫酸的滴加速度不能使中间产物温度大于25℃,发烟硫酸用量大于理论量10%~25%时,得到的五氟化磷纯度高于90%;六氟磷酸的加热温度对五氟化磷的产率和纯度均有影响,宜在148~153℃的范围内,加热时间应在30分钟左右;用工业酒精做冷凝介质得到的五氟化磷纯度最高只能到达94.03%,使用液氮做冷凝介质可以使五氟化磷的纯度达97.38%以上。

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