液氮

Methods:Fresh pig corneas were drilled down by 13mm-diameter trephine.

选择新鲜猪角膜，以13mm直径环钻制取角膜标本，依次放入四种冷冻保护液中冷平衡，"简化四步法"程序降温后放入液氮中保存，使用前40℃水浴复温。

As the models for VLE and enthalpy, MH-81' equation of state was applied to calculate the nitrogen scrubbing tower for two different operating modes by the tridiagonal matrix method. The calculate...

采用三对角矩阵方法，对两种不同工况的液氮洗涤塔进行了模拟，计算结果与设计值和实测值符合较好。

Results: The result is that PCNA positive appeared 6 hours after transplantation, increase unit time pass by, and reach peat at 1 to 2 week. After 2 weeks, PCNA positive of SMC in the tunica media begin to decline and recover to the level 6 hours after the operation at the 8 weeks.

本实验的第三部分，我们将于液氮固定的移植静脉段经组织匀浆后用于研究ECE和ET-1的表达情况，我们分别从mRNA水平和蛋白质水平，采用RT-PCR和免疫组织化学方法，对ECE和ET-1在移植静脉的表达情况进行分析。

PartⅡFeasibility of vitrifying normospermic samples using droplets without cryoprotectans Objective Discuss the feasibility of droplets to vitrify normospermic samples without cryoprotectants. Methods Normal prepared sperm were frozen by conventional freezing protocol and non-cryoprotectant freezing in droplets respectively. Freezing in droplets means freezing sperm in every 10ul drop. After thawing, compared the two groups'sperm mobilities.

第二部分微滴法无保护剂玻璃化冷冻正常精子的可行性探讨目的探讨在不添加冷冻保护剂的情况下微滴法玻璃化冷冻正常精子的可行性方法收集16份正常精液标本，上游处理后分别进行慢速冷冻和微滴法冷冻，微滴法即用拉细巴氏管将标本形成10ul的液滴，直接投入液氮内冻存，复苏后镜检其活动率。

RESULTS: Myocardial cells of the adult rats were preserved with ultrahypothermia liquid nitrogen storage and a sufficient number of bacilliform myocardial cells which met the quality standard were separated.

结果：采用成年大鼠心室肌细胞超低温液氮保存分离法，成功地分离出足够数量符合质量的杆状心肌细胞。

The cantharellus cibarius freezing technics was studied through analyzing the freezing curve at different temperature,freezing speed and juice leakage by liquid nitrogen flash freezer .

采用液氮速冻，探讨在不同冻结条件下鸡油菌的冻结温度曲线及冻结温度与解冻后鸡油菌的汁液流失率之间的关系。

In the case of cold induced brain injury with chalcone, the content of LPO and activity of SOD in animal b...

体内实验表明，免经液氮冷冻致脑损伤脑水肿后，其血清及脑组织中的SOD活性明显降低，LPO含量明显升高。

MethodThe extracts was prepared by grinding 800-1 000 mg fresh leaves of Clerodendron inerme and Vitex rotundifolia added with liquid nitrogen. The sample of total RNA was prepared by centrifugating the extracts added with reagent at 4 ℃, centrifugating at room temperature after ice bath and centrifugating at room temperature after adding reagent in order.

方法］取800～1 000 mg许树和单叶蔓荆的鲜叶，加液氮研磨后制成提取液，提取液加试剂4 ℃下离心，冰浴后室温下离心，依次添加试剂后室温下离心，即得total RNA样品。

ObjectiveTo explore the application of liquid nitrogen in the treatment of exfoliative gum lesion.

目的：探讨应用液氮低温治疗剥脱性牙龈病损的临床疗效。

The freezable water content in the frozen wet gluten was determined by differential scanning calorimetry, Compared with the conventional freezing process, more water was frozen during the ultrasound-assisted and liquid nitrogen freezing processes.

用差示扫描量热仪测定了不同冷冻环境下湿面筋蛋白中可冻结水的含量，结果可见：超声辅助冷冻和液氮浸渍冷冻的湿面筋蛋白中可冻结水的含量比传统冷冻过程的高。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新