液氮

Methods The cochlear samples cochlea of guinea pig and mouse were infiltrated with increasing concentrations of sucrose, followed by degassed embedding medium before final orientation and freezing. The sections were stained by hematein、eosin and immunohistochemistry. The morphology of the tissue sections was observed.

取1天龄大鼠、成年豚鼠、成年小鼠耳蜗标本经蔗糖快速浸透，用离心管定向并用新型组织包埋剂Tissue-Tek包埋，液氮中快速冷冻后在恒冷箱切片机中切片，然后分别做苏木素、伊红染色和免疫组化染色，观察组织切片的形态结构。

The results showed that the positive rates of RNA integrity in grind with liquid nitrogen group and in homogenizer without liquid nitrogen group were 68.42% and 29.79% respectively, and there was significant difference between these two groups; however, there was no statistic difference in positive rate of RNA integrity, OD260/OD280 ratio and 3-actin gene expression level between the drug-induced labor group and non-drug induced labor group.

结果发现，液氮研磨组分离完整RNA的阳性率高於匀浆器处理组，分别为68.42%和29.29%，两组比较有显著性差异；药物引产组及非药物引产组在分离完整RNA阳性率、胚胎组织RNA的OD260/OD280比值和胚胎组织β-actin基因表达量上均无统计学差异。

To investigate the method of RNA isolation from human embryonic tissues and the factors influencing the quality of RNA, the RNA from human embryonic tissues obtained with drug-induced labor or non-drug induced labor were isolated by using grind with liquid nitrogen or homogenizer without liquid nitrogen.

为探讨人类胚胎组织完整RNA的分离方法及其影响因素，采用液氮研磨及匀浆器两种制备组织匀浆方法，对不同引产方式的人类胚胎组织进行总RNA分离，并对RNA质量进行鉴定。

Objective To examine the cell viability and hemodynamic functions of the stented homograft valves preserved in liquid nitrogen.

文章摘要：目的检测经液氮保存的人同种带支架瓣膜的细胞活性和流体力学性能。

This study evaluated the clinical outcome of cryopreserved self-designed valved homograft conduit in the treatment of complex congenital heart disease.

回顾性分析自行制备和液氮深低温保存的同种带瓣管道治疗复杂先天性心脏病的临床应用效果。

Methods Cell viability of the stented homograft valve preserved in liquid nitrogen after 3 months of preservation （experimental group,n = 6） was examined using flow cytometer.

制作同种带支架瓣膜经液氮保存3个月后，利用流式细胞仪技术定量检测瓣叶细胞活性（实验组，n=6），采集新鲜同种瓣膜作为对照（对照组，n=6）。

Computational results about the pressure and temperature distributions along the cavitating hydrofoil were then compared to the experimental data of NASA to validate the model.

然后通过对液氮水翼汽蚀过程的计算，得到压力、温度等分布并与美国NASA实验数据做对比，验证了数值模型的准确性。

OBJECTIVE: To investigate the effects of liquid nitrogen cryopreservation on osteoblast immunogenicity.

目的： 探讨液氮冷冻对成骨细胞免疫原性的影响。

SEM result showed that the sample had uniform phase distribution and smooth surface at dried state as well as interpenetrate network structure at hydrogel state. It was seen from the swell ing behavior curves that groups T1, T2 and T3 swelled rapidly within 10-30 minutes, and formed elastic composite hydrogel pipes. In addition, the expanding ratio of inner and outer diameter of the tube was 20%-30% and 100%-120% with swell ing, respectively. The equil ibrium water content of the hydrogel pipes increased with increase of collagen composition. Three groups of samples were immerged in PBS solution contained 2 mg/mL lysozyme at 37 for 1 month, their nozzle cracked, their wall became thin and more transparent. And also, there was small floc deposited on the tube surface.

扫描电镜观察到液氮脆断的人工泪小管内部成分分布均匀，内外壁表面平整，冷冻干燥的人工泪小管断口呈凝胶态的互穿网络结构。3 组不同成分的人工泪小管在PBS 溶液中浸泡30 min 后均快速吸水溶胀，外径扩大100%～ 120%，内径扩大20%～ 30%，并且随胶原含量增高，溶胀速度增快，平衡溶胀率增大。3 组人工泪小管在37℃含2 mg/mL 溶菌酶的PBS 液中浸泡1 个月后，表面有部分细小絮状物，管口开裂，管壁变薄，透明度增加。

Methods Apply four ways: human baited net trap, catching with a net, light trap and indoor resting collection methods to catch the mosquitoes at the four habitat: urban areas, rice field, livestock and poultry shed, sad household, aid the environmental factors were recorded at the same time.

用人帐法、网捕法、灯诱法、人工小时法在城区、稻田、畜禽舍、住户4种不同生境捕获蚊虫，分类计数，放入液氮罐中带回实验室以备病毒分离。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新