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There was a linear positive correlation between the alkaline phosphatase activities and cell density.

液氮冷冻保存后复苏培养复层成骨细胞钙钴法碱性磷酸酶染色阳性率在45%以上,超微结构显示为高分化功能活跃成骨细胞。

It increases in the inclination angle range of 0° to 90°, and decreases in range of 90° to 180°, while the influence of the channel length on CHF is quite complicated.

在运用液氮冷却的场合中,特别是在一些需要紧凑结构设计的时候,窄缝常被应用。

To study this refrigerator below liquid nitrogen temperature, a low temperature rotary valve pulse tube refrigerator with liquid nitrogen precooler was built.

为了研究这种制冷机在液氮温区的性能,笔者搭建了带液氮预冷器的低温旋转阀式脉冲管制冷机并进行了测试。

Nothing comes out of the tailpipe except gaseous nitrogen, the main component of air.

这种取名为液氮 2000 ( LN2000 )的货车,其能源系统遵循的是古老的液氮气化释放能量的原理。

The umbilical cord blood solution, which the DMSO concentration is 1.0M, 1.5M and 1.75M,was confected. The solution was in equilibrium for 20 minutes. Then, the umbilical cord blood was completely immerged in liquid nitrogen for about 24 hours. Then, the umbilical cord blood was taken out and was warming in the thermostat water bath for 20 minutes. The fraction surviving of the umbilical cord blood was more than 80%.

先将含有细胞的培养液、0.9%的NaCl和2.0M的DMSO溶液配制成分别DMSO浓度为1.0M、1.5M、1.75M的脐带血干细胞溶液,将配好的玻璃化溶液置于4℃静置20分钟使脐带血干细胞的玻璃化溶液达到细胞内外溶液平衡,然后将其投入液氮中大约24小时左右,最后将液氮中的细胞取出37℃水浴复温,细胞存活率达到了80%以上。

In the reforming cryopreserving method, the fetal liver tissues were permeated and equilibnzed in cryoprotectant A and B for 20 minutes, respectively, and then put into liquid nitrogen quickly after cold equilibrium at liquid nitrogen steam for 5 minutes.

改良冻存法:胎肝组织在A液、B液中分别渗透平衡20min后,在冷冻保护液B液中经液氮蒸汽冷平衡5min后迅速入液氮冻存,1周后复苏,入DMEM培养液37℃孵育1h,胶原酶消化胎肝组织,分离、收集消化后的胎肝细胞培养观察。

The bending properties of China birch plywood were studied in normal condition, in low temperature (-196℃) and after being treated by Liquefied Nitrogen (-196℃, 48 h), its feasibility for application to insulated tank of LNG ship was analyzed in this paper.

研究了国产桦木胶合板在常温状态下、液氮低温(-196℃)状态下和液氮低温处理(-196℃, 48h)后的弯曲力学性能,并分析了其用于LNG船绝缘箱的可行性。

Sea perch embryos were cryopreservated in liquid nitrogen for 0.5~24h, 4 survival embryos were also obtained and one of them hatched, furthermore the fry without feeding survived for 3 days.

其中妒鱼胚胎成活4枚,孵化1枚,孵化后的妒鱼苗在没有投喂饵料的情况下存活了3天(在液氮中保存了28h);牙醉胚胎成活5枚,孵化4枚,孵化后其中l尾牙虾苗在没有投喂饵料的情况下存活了6天(在液氮中保存了24h)。

The essential differences were concluded between the explosive boiling and conventional boiling, also the differences between the explosive boiling in low temperature liquid and the one in normal liquid.

在实验研究基础上,数值模拟计算了液氮爆发沸腾过程中的温度变化,计算与实验结果基本吻合,能较好地反映液氮爆发沸腾过程中温度变化的基本规律。

Combined use of trypsin, hypertonic solution and chemical detergent is a better method to prepare acellular vascular matrix, and the vascular matrix be wellpreserved in liquid nitrogen.

液氮保存后1、2、3月扫描电镜观察与新鲜标本无明显差别。结论酶、低渗溶液、化学除垢剂联合法是制备脱细胞血管基质的较好方法,制得的脱细胞血管基质可放入液氮中保存。

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