液氮

By partially thawing the prefrozen samples and refreezing them in liquid nitrogen before the final liyophilization, we prepared biomimetic multilayered scaffolds.

采用-20℃冷冻后，部分熔融再液氮重冻的预冻方式，冷冻干燥制备了厚度约2mm的仿生多层软骨支架。

The figure of the broken cases of liquid nitrogen container is 15 of which 7 are stevedores' damage.

液氮集装箱的破箱数量是15，其中7箱是工残。

Are specializing in the design, production of industrial equipment and auxiliary gas, dissolved acetylene equipment (seamless, welding, dissolved acetylene, liquefied petroleum, natural gas and other gas cylinders) Chemical Equipment and accessories, all kinds of gas Filling Units, bus high-purity, specialty, medical gas cylinder seamless vacuum devices do bath, stainless steel PTFE metal software, bellows, industrial gas high, medium pressure valves, the fittings in the gas, industrial gases pallets, providing liquid oxygen, liquid nitrogen, liquid argon, liquefied petroleum, liquefied natural gas, two ice machines, to undertake air separation, acetylene equipment¨and renovation project as well as standard gas, mixed gases, specialty gases, high purity gases configuration and installation industry.

丹阳市荣发气体设备有限公司是专门设计，生产工业气体设备及辅机，溶解乙炔成套设备（无缝，焊接，溶解乙炔，液化石油，天然气等气瓶）化工设备及配件，各种气体充灌台，汇流排高纯，特种，医用气体无缝气瓶真空干澡装置，不锈钢四氟金属软件，波纹管，工业气体高，中压阀门，各中气体配件，工业气体集装架，提供液氧，液氮，液氩，液化石油，液化天然气，二冰机，承接空分，乙炔设备建站及改造工程以及标准气体，混合气体，特种气体，高纯气体的配置工业及装置等。

"Feihang" brand man-made boards produced by the Group include medium density fibre boards, composite floor boards, joinery boards, container bottom boards and so on.The Group has become the largest container bottom board-produced ernterprise."Bashan" brand metal wires, wire mesh sreeens, filter screens are widely used for many fields,such as aerospace, autos manufacture, metallurgy, chemical industry, machinery, electronics and so on."Hangkong" brand liquid nitrogen living things vessel line products has obtained State Silver Medal for three times. The company has produced different specifications and types of cryogenic containers.

集团生产的&飞航&牌人造板材包括中密度纤维板、复合地板、细木工板、集装箱底板等100多个品种，成为国内最具规模的集装箱底板生产企业；&巴山&牌金属丝、金属筛网、滤网等产品广泛用于航空航天、汽车制造、冶金、化工、机械、电子等行业；&航空&牌液氮生物容器系列产品三次被评为国家银质奖章，生产出3升到400升30余种不同规格和型号的低温容器，广泛应用于超低温冷冻技术和畜牧、医疗、科研等不同领域。

METHODS: Open chest operation was performed on the anesthetized rats to take out their hearts. The remaining blood was washed, and the large vessels, atriums and right ventricle were removed. The left myocardium was cut into two parts, then put in the DMEM tube containing 600 mL/L of glycerol, frozen at 4℃ for 30 minutes, at -20℃ for 30 minutes, and at80℃ for 30 minutes. Then they were stored in the liquid nitrogen at196℃.

麻醉大鼠开胸后，迅速取出心脏，置于冷Krebs-Henseleit液中，洗去残存的血液，弃去大血管、心房和右心室，将左心室肌剪成两块，然后置于含600 mL/L甘油的DMEM的冻存管中，程序冷冻，即4℃30min，-20℃30 min，-80℃30 min，最后保存于-196℃的液氮中备用。

He devised a system of liquid nitrogen delivery through insulated cannulas that could be placed within the substance of the liver.

他设计出了一套用绝热导管输送液氮并释放到肝实质的系统。

The lucky winner of the prize promoting the revamp of New Scientist magazine won't be able to collect the award until death when he or she will be cooled to a temperature at which decay of the body stops and then suspended in liquid nitrogen in a state known as cryonic preservation.

届时获奖者的身体将被冷却到足以停止身体腐坏过程的低温，然后被悬浮在液氮中，进入冷冻保存状态。

The total static loads and dynamic loads are summed as 4.692W and 38.4W, respectively. And comparison between the double-wall cooling method and the cooling tube method for the 77 heat shield has been discussed to provide the thermal parameters for flow process simulation. The critical thermal parameters of the main components of the QWR cooling system were determined for parallel arranged QWR cryostats. The mass flow and time for cool-down of the QWR to 4.2K was also estimated.

本文在详细描述恒温柜冷却系统的基础上，详细计算了4.2K冷质量与77K冷屏的辐射漏热，同时，还对恒温柜内部支撑系统计算了变物性下的导热漏热，得出了恒温柜系统的静态和动态热负荷分别为4.692W和38.4W，并分析比较了液氮冷屏采用夹层冷却方式与铜管缠绕冷却方式的优缺点，为流程模拟的参数确定提供了依据。

In the aspect of research and development of HAS, the United States, Japan, united Kingdom, France and Italy recently tried tk use lower eucaryote, espesially yeast expression system to express HAS, obtained outstanding progress.

HSA的表达及工程化：将含HSA基因的酵母转化子单个菌落分别接种，并且分别进行甲醇诱导表达；将酵母表达的HSA蛋白质经SDS-PAGE电泳鉴定，筛选出多拷贝整合和高水平表达HSA菌株；将高表达HSA工程菌加保护剂后分别在液氮和－70C°低温冰箱内保存。

On-hoard determination of picoplankton is preferred, if possible. Otherwise, it is better to use paraformaldehyde or the mixture of paraformaldehyde and glutaraldehyde as fixatives, the samples stored in liquid nitrogen should be analyzed as soon as possible.

如有可能，应该尽量现场测定微微型浮游生物样品，否则建议采用多聚甲醛或其与戊二醛的混合物作为固定剂，将样品保存于液氮中，待返回实验室后尽快进行测定。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新