液氮

Objective To study the difference of healing process of reconstructing the knee cruciate ligaments with allograft tendons which were treated by program freezing and －80℃ deepfrozen.

目的 从实验角度观察程序冷冻液氮保存和－80℃深低温保存方法处理同种异体髌腱后重建膝关节交叉韧带的愈合过程并比较其差异。

Department of Orthopaedics,Qingdao Municipal Hospital,Qingdao 266000,ChinaAbstract:Objective To study the difference of healing process of reconstructing the knee cruciate ligaments with allograft tendons which were treated by program freezing and －80℃ deepfrozen.

目的 从实验角度观察程序冷冻液氮保存和－80℃深低温保存方法处理同种异体髌腱后重建膝关节交叉韧带的愈合过程并比较其差异。

Methods Reconstruct the ACL of the knee with the BPTB of rabbits which had been preserved for 2 weeks with －80℃ deepfrozen or liquid nitrogen deepfrozen after program freezing,and the cells poison response,cells activity,the maximum load and morphologic changes of the grafts were observed to compare the two allografting groups each other and compare them with the autografting group.

将兔的1/2骨髌腱骨复合体经-80℃深低温保存和程序冷冻液氮保存2周后观察冻存变化差异并行同种异体移植重建前交叉韧带，分别于术后3周和8周观察细胞毒反应、细胞活性、最大载荷和形态学变化等指标进行比较并与自体移植组对照。

The present invention is characterized in that the ammonium metatungstate powders and the cobltic-nitrate powders are respectively dissolved in the water and scattered in the liquid nitrogen to be pre-frozen using a nitrogen spray gun after being mixed and defecated, and then are placed in a freezedryer to be dried vacuumly and finally the powders are obtained. The nanometer powder i.e. the nano tungsten-cobalt composite powder is obtained by reducing the freeze-dried mixed powder in the atmosphere of hydrogen.

该微晶硬质合金的制备方法特征为：将偏钨酸铵粉末与硝酸高钴粉末分别溶于水中，混合澄清后使用氮气喷枪，将其分散在液氮中预冻后置于冻干机中进行真空干燥得到粉末；在氢气气氛中对冻干的混合粉末进行还原得到纳米粉体，即钨钴纳米复合粉体。

An adiabatic system is designed to obtain the cooled circulating condition for the superconducting magnet. The system is comprised of vacuum adiabatic mti1ayer, copper thermal shield, snake vent-pipe and so on, which elided the complicated frame of liquid nitrogen thermal shield outside of the inner-cryostat, simplified technical structures, and achieved favorable adiabatic effect.

为了获得有效的超导磁体运行的低温环境，研制了一套采用真空多层绝热、铜辐射冷屏、蛇形排气管结构形式的绝热系统，省去了传统的在内杜瓦外面添加液氮屏的结构，简化了工艺结构，操作方便，绝热效果良好。

The critical heat fluxes in narrow channels are experimentally investigated in the present study.

本文在实验的基础上对窄缝通道中液氮的临界热流密度进行了实验研究。

Methods By lyophilizing the scaffolds which were prefrozen at -20℃ and in liquid nitrogen successively, we prepared double-layered spongy scaffolds.

采用先后于-20℃和液氮中冷冻的预冻方式，冷冻干燥法制备双层支架。

Methods Ground dragon was respectively processed by traditional processing technology and cold-processing technology.The latter was done by the liquid nitrogen rapid freezing technology and lyophilizing technology.By means of qualitative and quantitative tests,SDS-PAGE and the software of ImageTool were applied to analyzing the difference of protein components in ground dragon from the two processing techniques.Fibrin plate method and thrombolysis test in vitro were carried out to determine the difference of the two processing techniques for the fibrinolytic activity.Micro-observation and MTT methods were used to assess the differences of antihepatic cancer activity between the traditional and the cold-processing groups.

方法地龙的炮制采用传统与低温两种工艺，低温炮制采用液氮快速冷冻与冷冻干燥技术；采用SDS-PAGE与ImageTool软件，对两种炮制工艺的地龙蛋白质组成的差异进行定性与定量分析；应用纤维蛋白平板法与体外溶栓试验，定性与定量分析两种炮制工艺的纤溶活性的差异；应用显微观察与MTT法，对两种炮制工艺体外抑制人肝癌细胞增殖活性进行定性与定量比较分析。

In 1999, Kuwayama left the boine world to became head of research at Tokyo's Kato Ladies Clinic, which says it performed nearly 18,000 in-itro fertilization cycles last year. He soon deeloped a quick-freezing method using liquid nitrogen that reduced crystallization and lowered the need for alcohol.

在1999年，Kuwayama离开牛厂去成为东京Kato Ladies Clinic的研究主任，它说，他去年做了18000例体外受精实验，然后不久他发展了用液氮快速冷冻的方法，这种方法不仅降低了结晶而且也减少了对乙醇的需求。

The sample was extracted by deionized water. The extract was cleaned up and concentrated on a SPE column, then eluted by methyl-tert-butyl ether. The elution was concentrated under nitrogen and redissolved to 1 mL.

样品中24种目标物用蒸馏水浸出后，经固相萃取小柱富集净化，再用叔丁基甲醚洗脱，洗脱液氮吹浓缩定容后用气相色谱－质谱法测定。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新