液囊

Firstly , the V4 stock was purified three times by plaque formation. It's difficult to see clear plaques in CEF cells due to the avirulent characteristic , so we treated the V4 stock with trypsin and then allantoic Fluid and Mg2+ were added in the layer gel. In this case, we could see small plaques occasionally, but not stably.

本实验首先对实验室保存的V4株进行了三轮的蚀斑纯化，由于V4株属无毒株，其在CEF细胞中很难形成可见的蚀斑，因此我们采用了用胰酶处理V4病毒，并在上层胶中添加尿囊液及Mg2+，这样可以偶尔产生可见的蚀斑，但实验结果重复性很差。

The amniotic fluid and allantoic fluid of chicken embryo have been analyzed and the resonances of most substances in them were assigned by 1-dimention and 2-dimention Nuclear Magnetic Resonance methods. This work could be the basis of quantitative analysis of metabolites and studying the metabolites changing between the amniotic and allantoic fluid during the hatching process. Also, it provided a new way to study the process of embryo developing.

用一维及二维液体高分辨核磁共振(Nuclear Magnetic Resonance，NMR)方法综合分析了鸡胚羊水和尿囊液的成分，对其中的大多数谱峰进行了归属，发现了一些未经报道的小分子代谢物，为研究胚胎发育过程中各个胚囊中体液的交换和小分子的代谢以及进一步对发育过程中代谢物含量变化的定量研究打下了基础，并为研究胚胎发育的过程提供了新的思路。

The avian influenza virus RNA was directly extracted and purified from chicken em-bryo allantoic fluid. According to published gene sequence,a pair of specific primers to the nucleoprotein gene of AIV was designed and synthesized. After that,the NP cDNA was amplified by RT-PCR .Then the complete NP gene was sequenced.

直接从鸡胚尿囊液中提取H9N2亚型禽流感病毒的RNA，并根据已发表的A型流感病毒株的核苷酸序列，设计了1对特异引物，采用RT-PCR技术成功地扩增了AIV的NP基因；将NPcDNA克隆后进行了序列测定。

The PCR products appeared to be approximate 600 bp, the expected size. The allantoic fluid of infected embryonating eggs of 6 IBV reference strains and 6 field strains were amplified by RT-PCR using three pairs of primers of 1.7, 0.2, 0.6 kb on same and different condition, 3, 5, 9 strains of IBV were amplified respectively. While 12 different sentypes of the 12 IBV strains can be divided in 6 genotypes.

用1.7、0.2、0.6 kb 3对引物对6个IBV毒株和6个分离株的含毒尿囊液在相同和不同条件下进行RT-PCR，结果3对引物分别扩增出3、5、9株IBV，同时可将不同血清型的12个IBV株分成6种基因型。

The main points included:the selection of the best level, the puncture point, angle and depth of needle; exhaustedthe allantoic fluid, pumped into pure ethanol and exhausted it after 5~10 minusts.

要点包括选择最佳层面，穿刺点、进针角度及深度；抽净囊液，注入纯无水乙醇保留5～10 min抽净。

The best survuval rate of each stage thawed-embryos existed in groups of Zygote EDT3O-two-step method with I mm equilibration(66%), of Morulae EDT3O-two-step method with I mm equilibration(94%) and EDT4O-two--step method with 0.5 mm equilibration(94%), of Blastocyte EDT3O-two-step method with I mm equilibration(91%) and EDT4O-one-step method with I mm equilibration(91%).

总之，我们的研究表明，DET玻璃液冷冻方法是简单、经济而又高效的，冷冻小鼠各阶段胚胎都能获得较高的存活率，其中受精卵的EDT30二步法1分钟平衡组(66％)、桑椹胚的EDT30二步法1分钟平衡组(94％)和EDT40二步法0.5分钟平衡组(94％)、囊胚的EDT30二步法1分钟平衡组(91％)和EDT40一步法1分钟平衡组(91％)冷冻效果最好。

Results The main CT features of adrenal neuroblastoma in children included:huge and irregular mass(25 cases),the density is more even(18 cases),calcific

结果：儿童肾上腺神经母细胞瘤的CT主要表现有大的不规则肿块25例，密度较均匀18例，钙化12例，囊变或坏死8例，出血4例，越过中线22例，包绕腹膜后大血管20例，腹膜后淋巴结肿大18例，2例肝转移，8例腹腔积液，2例盆腔转移。

The diffusive XGPN shows cystic-solid multi-occupying with the whole kidney involved and kidney renal pelvis and calices enlarged. The lesions were extensive with unclear border which made the perirenal space dim renal fasciae thicken and with the abscess formation of psoas and abdominal wall. The localized XGPN shows mass in the renal parenchyma with low density clear border after enhancement and rim enhancement.

弥漫型累及全肾，肾影增大，肾盂肾盏扩大积液，肾实质幕粥发囊实性占位，病变分界不清，向肾外扩展，肾周间隙模糊，肾筋膜增厚并累及腰大肌，腹壁脓肿形成；局灶型表现为肾实质内局限性占位，病灶区呈略低密度灶，增强后边界清晰，病灶内见环形强化，而略低密度灶者强化较明显，但仍低于肾实质。

Ingredients:water,glycerin,caprylic/capric triglyceride, cetyl alcohol ,propylene glycol,glyceryl stearate,panthenol,betaine dimethicone,simmondsia chinensis, rosa rugosa flower extract,allantoin,carbomer,parfum,triethanolamine,tocopheryl acetate,methyl paraben,propyl paraben,creatine,tetrasodium edta,phenoxyethanol,caprylyl glycol,sorbic.

成分：去离子水，甘油，辛酸/葵酸三甘油酯，鲸蜡醇，丙二醇，硬脂酸甘油酯，泛醇，甜菜碱，聚二甲基硅氧烷，霍霍巴油，玫瑰花提取液，尿囊素，卡波姆，香精，三乙醇胺，生育酚乙酸酯，对羟基苯甲酸甲酯，对羟基苯甲酸丙酯，肌酸，乙二胺四乙酸四钠，苯氧乙醇，辛二醇，山梨酸。

8Neoplastic bone or neoplastic calcified cartilage: the former demonstrate ill-defined patched, radial or conglomeratic high density area in CT; the latter demonstrate spotty or circled high density in CT.

MR检查显示软组织肿胀I度2例，n度6例，nl度6例。3例显示脓肿样囊腔，在MR DWI上均呈明显高信号。1例显示脂液平面。1例显示软组织肿块。14例MR检查均未见软组织内气体。70例恶性骨肿瘤病人中，54例行CT检查，49例行MR检查。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。