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Results:(1)NSCs form typical neurospheres under adequate concentration in vitro, which are immunoreactive to Vimentin. Typically and terminally differentiated mature neural cells could not be found without the stimulus of mitogen or only under NSCs self-regulation and self-induction;(2)NSCs derived from hippocampus maintain the character of stem cells much longer with better biological behavior; NSCs passed to the 2-3 passage are the best to graft since they have not differentiated;(3)NSCs cultured in vitro could self-regulate and differentiate into neurospheres and progenitors positively immunoreactive to specific antibodies representing neurons, astrocytes, oligodendrocytes and Schwann cells;(4)There are widespread synaptic contacts between various kinds of descendent clones and cells;(5)Neurospheres could be formed without the stimulus of mitogen when NSCs and OECs are cocultured. Many neurospheres and cells immunoreactive to Vimentin, GFAP, MAP2, 02, p75NGFR, GFAP, S-100, Synaptosis, Vimentin, Tau (Tau is only positive in cocultureof HNSCs+HOECs) could be found;(6)The supernatant fluid triturated from adult rat spinal cord stimulates NSCs to differentiate into neurons, but do not terminally differentiate;(7)Fibroblasts and O4 oligodendrocytes are not supported to grow under this culture medium.Part II: Isolation, culture and identification of rat and human olfactory ensheathing cellsOlfactory ensheathing cells/glials are the most powerful cells to enable the regeneration of axons in the central nervous system.

结果表明:①在适宜的浓度体外培养条件下,NSCs能形成典型的神经干细胞克隆球,Vimentin免疫荧光染色阳性,单靠丝裂原刺激或NSCs自我调节和分化诱导,不会产生典型的终末分化的成熟神经细胞;②海马源性的NSCs维持干细胞特性的时间更长,生物学特性更优;③传至第2~3代的NSCs尚未分化时移植最佳;④体外培养的NSCs能自我调控分化为神经元、星形胶质细胞、O2少突胶质细胞、雪旺氏细胞染色阳性克隆球和前体细胞;⑤各种子代克隆球和细胞存在广泛的突触联系;⑥NSCs与OECs联合培养时,不需丝裂原刺激即能形成克隆球,获得大量Vimentin、GFAP、MAP2、O2、p75NGFR、GFAP、S-100、Synaptosis、Vimentin、Tau(Tau只有人HNSCs+HOECs联合培养时出现阳染)染色阳性的克隆球和细胞;⑦脊髓研磨后的上清液刺激神经干细胞向神经元方向分化,但并不出现终末分化;⑧本研究培养条件不利于成纤维细胞、O4生长。

The method for manufacturing the filter includes the steps of immersing a supporter in a suspension in which the powder of the fraipontite mineral and the powder of an inorganic substance as a binder are dispersed as the suspensoid, and drying the supporter impregnated with the suspensoid, thereby forming an inorganic material layer fixed to the surface of the supporter.

制造该过滤器的方法,包括将载体浸渍在悬浮液中的步骤,在该悬浮液中将丝锌铝石矿物粉末和作为粘结剂的无机物质粉末分散成悬浮肢体,和干燥用该悬浮胶体浸渍过的载体的步骤,从而制成固定到载体表面上的无机材料层。

Used for filtering of rotated chipping, grinding, honing, washing, drawbench, Stretching liquid, plating, milling, hardening liquid and oil regenerating.

转削、磨削、钻孔、搪磨、洗涤、拉丝机、拉伸液、电镀、研磨、硬花液、油再生之过滤。

When criterion of 80% urinary red cell dysmorphism was assigned as diagnostic for glomerulonephritis, and the causes of hematuria agreed with the clinical, histologic, and laboratory diagnosis, the examination of urinary red cell morphology had a diagnostic sensitivity of 93.3% and specificity of 100% in the 38 hematuria children.

若以尿液中变形红血球有大於80%以上做为肾丝球出血之标准,且以临床症状,组织,生化及放射线学等实验室检查做为佐证时,在本研究的结果中显示;以尿液中红血球形态做为血尿来源之诊断,其敏感性约为93.3%,而其专一性可达100%。

In this paper, the influence of liquid loadage, rotating speed, xylose concentration and organic nitrogen source on xylose fermentation by Candida shehatae CICC 1766 to produce ethanol were investigated. Besides, ethanol tolerance of Candida shehatae CICC 1766 was studied.

研究了装液量、转速、木糖浓度、有机氮源等条件对休哈塔假丝酵母CICC 1766发酵木糖生产乙醇的影响,探讨休哈塔假丝酵母的乙醇耐受能力。

The process is carried out by rubbing into filament raw materials, digesting in plasticizing liquid of sodium hydrate and sodium silicate at 100-130degree for 1-2hrs to obtain plasticized raw material, high-speed agitating plasticized raw materials with non-metallic minerals, octadecanoic acid and silicon oil at 100-130degree for 15-45hrs, coating and drying to obtain the final product.

先用农用搓揉机直接搓揉成丝状物料,然后丝状物质在含氢氧化钠和硅酸钠的塑化液中于100~130℃下蒸煮1~2小时,得到塑化物料,最后将塑化物料同改性非金属矿粉、硬脂酸和硅油在100~130℃下高速搅拌15~45分钟进行包覆处理,干燥后便得到目标产物。

The coarse grain in cast strip is produced on the condition that cool speed is not fast enough to overcool aluminum melt in whole cast area, and only provide proper temprature gradiant to keep cellular grain growing up.

铸轧区内铝液与铸轧辊接触瞬间,当铸轧辊冷却强度不足以使整个断面铝液同时产生较大的过冷,而仅能保持胞状晶生长所需的液体温度梯度时,铸轧板就会出现粗大晶粒;根据传热学原理,分析了具体生产条件,提出增大铸轧辊冷却强度、降低前箱温度以及添加铝钛硼丝等防止铸轧板产生粗大晶粒的措施。

Root tip generally became shorter and thicker because the aqueous extracts of B. pilosa could accelerate periclinal divisions and increased cell layers of the maturation zone and inhibit cell elongation.

三叶鬼针草水浸提液抑制了细胞有丝分裂的正常进行,并随着水浸提液质量分数的升高,分裂期细胞数目下降,微核率逐渐升高。

Result In PDA culture medium to add 10%-20%, 20%-30%, 20%-40% of the Tenebrio molitor Linnaeus extracts, the mycelium of Russula, Coprinus comatus, Pleurotus and Pleurotus geesteranus growth had increased markedly, but the mycelium of Hericium erinaceus had not significantly growth, and when the Tenebrio molitor Linnaeus extracts concentration was more than 20%, it was inhibition.

结果]在PDA培养基中加入10%~20%、20%~30%、20%~40%的黄粉虫粪提取液时分别对红菇、平菇和鸡腿菇、秀珍菇菌丝丝生长具有明显的促进作用,但对猴头菇菌丝生长影响不显著,且黄粉虫粪提取液加入量达到20%以上时出现抑制现象。

The experiment results shown that: 1 the smaller the pH value, the smaller the use amount of alkaline metal and the alkali earth metal electrolyte demulsifier, when the pH value was 3, the waste fluid could be demulsified no any demulsifier and the use amount of the demulsifier did not increase remarkably along with the wiredrawing fluid concentration increased. 2 Using polymeric ferric sulfate as coagulant demulsifying, when the pH value was 8.4, the best adding amount was 40 mL per liter wastewater and the best precipitation time was 4 hours. Then the COD concentration was 480 mg/L, reaching the water quality requirements to enter the sewage treatment plant.

pH值越小,碱金属和碱土金属电解质破乳剂的用量越小,pH值为3时,无须加入任何破乳剂即可破乳;破乳剂用量并不随拉丝液浓度的增大而显著增加。2)用混凝剂聚合硫酸铁破乳,在pH值8.4时,最佳投加量为40 mL/L,最佳沉淀时间4 h,处理后上清液COD浓度为480 mg/L,能满足进入污水处理厂水质要求。

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