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METHODS: The different concentrations of natural monoclonal antikeratin antibody IgM 3B4 were incubated with Candida albicans yeast phase suspension on condition that profited germ tube formation of Candida albicans to observe the action of natural monoclonal antikeratin antibody IgM 3B4 to Candida albicans germ tube formation.And the different concentrations of natural monoclonal antikeratin antibody IgM 3B4 were incubated with the mixed suspenions of Candida albicans yeast phase with malpighian cells, human buccal epithelial cells, endothelial cells of fetal umbilical vein, respectively, to observe the action of natural monoclonal antikeratin antibody IgM 3B4 to the adhesion of Candida albicans to malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein.

将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4分别与白念珠菌酵母相悬在有利于芽管形成的条件下共同孵育,倒置显微镜下计数白念珠菌总数以及白念珠菌芽管数;将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4与白念珠菌酵母相和人角质形成细胞混悬、白念珠菌酵母相和人口腔黏膜上皮细胞混悬、白念珠菌酵母相和胎儿脐静脉内皮细胞混悬共同孵育,分别计数人角质形成细胞、人口腔黏膜上皮细胞、胎儿脐静脉内皮细胞上粘附的白念珠菌数。

Keratin antibody IgM 3B4 specifically against Candida albicans in vitro. METHODS: The different concentrations of natural monoclonal anti??keratin antibody IgM 3B4 were incubated with Candida albicans yeast phase suspension on condition that profited germ tube formation of Candida albicans to observe the action of natural monoclonal anti??keratin antibody IgM 3B4 to Candida albicans germ tube formation.And the different concentrations of natural monoclonal anti??keratin antibody IgM 3B4 were incubated with the mixed suspenions of Candida albicans yeast phase with malpighian cells, human buccal epithelial cells, endothelial cells of fetal umbilical vein, respectively, to observe the action of natural monoclonal anti??

将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4分别与白念珠菌酵母相悬在有利于芽管形成的条件下共同孵育,倒置显微镜下计数白念珠菌总数以及白念珠菌芽管数;将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4与白念珠菌酵母相和人角质形成细胞混悬、白念珠菌酵母相和人口腔黏膜上皮细胞混悬、白念珠菌酵母相和胎儿脐静脉内皮细胞混悬共同孵育,分别计数人角质形成细胞、人口腔黏膜上皮细胞、胎儿脐静脉内皮细胞上粘附的白念珠菌数。

METHODS: 24 rabbits were divided into four groups: control group; rapid pacing group; adenosine perfusing group; selective adenosine receptor antagonist perfusing group. The index of observed: time of repolarization in monophase action potential (APD90); the active level of A1 adenosine receptor; the level of free calcium in the atrium cells and the ability of atrium cell to release calcium.

新西兰白兔24只,随机分为4组:①对照组:常规克氏灌流,②腺苷灌流组:灌流中加入2umol/L的腺苷,③心房快速起搏组:常规克氏灌流+心房快速起搏,④腺苷受体拮抗剂干预组:克氏+DPCPX(100nmol/L)灌流+心房快速起搏;观察指标:①起搏前后心房肌单相动作电位复极时间的变化;②起搏组和对照组的心房肌腺苷A〓受体活性对比;③心房肌细胞内游离钙含量以及氯化钾激动后钙释放能力对比。

METHODS: Rat bilateral olfactory bulbs and olfactory mucosa at 1/3 nasal septum were obtained, sliced, digested in trypsin, and made into monoplast suspension. At 1×109/L, cells were incubated in uncoated 25 cm2 culture flask. At 18-20 hours, cell suspension was moved into another uncoated 25 cm2 culture flask (the first differential adhesion). At 24 hours, cell suspension was moved into a poly-D-lysine-coated 25 cm2 culture flask or poly-D-lysine-coated 6-well culture plate (the second differential adhesion). At 48 hours, parenchyma cells were removed after a half of medium was changed.

完整取大鼠双侧嗅球及剪取鼻中隔后1/3嗅黏膜,剪碎后胰酶消化,制成单细胞悬,按1×109 L-1密度接种于未包被的25 cm2玻璃培养瓶中,18~20 h后将细胞悬转移至另一未包被的25 cm 2玻璃培养瓶中(第1次差速贴壁),24 h后再将细胞悬转移至经多聚右旋赖氨酸包被的25 cm2塑料培养瓶或经多聚右旋赖氨酸包被的6孔培养板中(第2次差速贴壁),接种培养48 h后半量换以去除杂细胞。

Results There were hemodynamic changes of double kidneys in all 120 cases of pregnant women, thereinto, the signs were seen for nephrauxe, hydronephrosis (including 35 cases with hydrops of double kidneys, 12 cases with hydrops of left kidneys ) and sparate collecting system of kidney in various degree in 108 cases, otherwise, there were no visible ultrasonic symptom on hydrops of double kidneys, but low-grade nephrauxe in 12 cases.

结果 120例中孕妇双侧肾脏血流动力学均发生改变,108例受检孕妇中均有不同程度的肾脏体积增大、肾脏集合系统分离、肾脏积,其中35例双侧肾脏积,12例仅表现为左侧肾脏积;120例中有12例双侧肾脏未见明显积声像,但有轻度的肾脏体积增大。

Furthermore,using oxaldehyde,make the emulsion crosslinked and form a kind of net structure,thus a new kind of water-resistant adhesive is created.

通过醋酸乙烯酯共聚改性的方法,用醋酸乙烯与丙烯酸系单体共聚,采用无皂乳聚合,然后用乙二醛交联,使乳形成网络结构,制备了一种新型耐水性胶粘剂,研究了共聚单体对乳性能的影响,并采用DSC、透射电镜等对乳结构及性能进行了测试。

The magnetic properties of carbonyl iron particle s were characterized by VSM;the apparent viscosities and the magnetic field-induced shear stresses of the magnetorheological fluids above were investigated by an advanced rheometric expansion system;the properties of MR damper were tested by an electro-hydralic servocontrol testing system.

研究了一种新型MR和MR阻尼器;用振动样品磁强计测试了MPS-MRF-25羰基铁粉的磁性能;用磁流变仪测试了MR的表观粘度和磁致剪切应力;用静置沉降法分析了MR的抗沉降稳定性;用电伺服材料性能试验机测试了MR阻尼器的性能。

Make up 40% sugar juice now, using sugar volume and raw material to compare is 1: 0.7, 0.2% citric acid are added in sugar juice, because watermelon skin organization is harder, can appear candy law to heat with boil make, cast the watermelon skin that cent has cut into to boil make, it is OK to begin big a crucial moment heats, make its fast evaporate moisture, boil raw material to become transparent all the time, when sugar juice concentrates ceaselessly and showing syrup, should be to use small fire to undertake heating, lest produce anxious saccharify to make product darkly Brown, determine sugar juice chroma is achieved 70% when should stop to heat.

现配制40%糖,用糖量与原料比是1:0.7,在糖中加入0.2%柠檬酸,因西瓜皮组织比较坚硬,可用一次透糖法加热煮制,把分切好的西瓜皮投进一起煮制,开始可以大火候加热,让其快速蒸发水分,一直煮到原料变得透明,糖不断浓缩而呈糖浆时,应是用小火进行加热,以免产生焦糖化使产品呈黑褐色,测定糖浓度达到70%时就应停止加热。

Armigera and M. separata mid-gut juice, but, other less than 60kD proteins also can be got by M. separata mid-gut juice .4 Many Bt Cry1AcE toxins can be got by the method of precipitate and resolve, which have high purity.

在相同的时间下,粘虫中肠消化蛋白酶活性比棉铃虫中肠消化要高;在37℃条件下,棉铃虫中肠消化经过30和60min将130kD的Bt CrylAc原毒素酶解为60kD的毒素,而粘虫中肠消化经过30和60min对Bt CrylAc原毒素酶解作用,除得到60kD的毒素外还得到了40kD和分子量更小的蛋白。

2 The analysis step by 125mL separatory funnel, a fartoo 0.1mL cadmiun forcultivation added liquid (equivalent 1 μ g cadmiun) and 50mL acetate (4%), and the other one is to join the 50.0mL sample lixivium, separatory funnel and 2mL in the seignette solution,-amoxycillin sodium hydroxide solution and potassium cyanide-2mL hydroxylammonium hydrochloride solution, each adding a reagent shake after.

9.2.2 分析步骤取125mL分漏斗两只,一只加入0.1mL镉标准使用(相当1μg镉)及50mL乙酸(4%),另一只加入50.0mL样品浸泡,分别向分漏斗中各加2mL酒石酸钾钠溶,10mL氢氧化钠-氰化钾溶及2mL盐酸羟胺溶,每加入一种试剂后均须摇匀。

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But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊!不用提了。提到肉,真是糟透了。

Tristan, I have nowhere to send this letter and no reason to believe you wish to receive it.

Tristan ,我不知道把这信寄到哪里,也不知道你是否想收到它。