涂片标本

Then take the contrast of the two antimicrobial susceptibility testing and the extent of the coincidence of the selecˉtionof the NIT and LEU bacteriuria testing.Results In96patients of the acute urinary infection,90positive sample of both NIT and LEU,and6NIT-negative LEU-positive sample,and through culture both samples examine the bacˉteria.

结果 96例急性泌尿道感染患者尿液标本中，NIT和LEU均阳性样本90份，NIT阴性、LEU阳性样本6份，经培养均检测出病原菌；LEU00/μl的样本直接涂片找细菌阳检率低。

This image was composed to depict the appearance of a 4X field with approximately 1400 cells. It is to be used as a guide in assessing squamous cellularity of conventional specimens.

此图像提示在一4倍视野中细胞大约有1400个细胞，在传统涂片标本中，此作为评估鳞状细胞数量的一个指标。

Through this issue, we found that with 2% NaOH-NALC digested within 10 minutes in the rapid decomposition of the viscous sputum, in ensuring the effectiveness of digestion at the same time not too much because of the role of NaOH to kill bacteria (time to 20 minutes), and would not affect the role of magnetic particles; quantitative simulation of self-made through the application of clinical samples, we compared the acid-fast stain and auramine O staining of the detection limit, auramine O fluorescence staining against the backdrop of a clear dark green light acid-fast stain of Mycobacterium tuberculosis than the white background of the red eye-catching TB is more capacity, auramine O staining in the detection limit of 0.05 to 0.001 between Maxwell, higher than the acid-fast stain of Michael's 0.05 units, The former is more sensitive; In addition, the accumulation of magnetic nanoparticles of the samples after the smear, in the microscope show the number of TB was significantly higher than that of the specimens has not been enriched.

通过本次课题，我们发现用2%NaOH-NALC消化液可在10分钟内迅速裂解痰液的粘性介质，在保证消化效果的同时不会由于NaOH过强的作用杀死细菌（时间控制在20分钟内），亦不会影响磁性颗粒的作用；通过应用自制定量模拟临床标本，我们比较了抗酸染色和金胺O荧光染色的检出限，金胺O荧光染色黑暗背景下发出明显绿色亮光的结核杆菌较抗酸染色白色背景中的红色结核杆菌更容醒目，金胺O荧光染色的检出限在0.05~0.001个麦氏单位之间，高于抗酸染色的0.05个麦氏单位，前者敏感度较高；另外，经过磁性纳米颗粒富集作用后的标本涂片，在镜下显示其结核杆菌数量明显高于未经过富集的标本。

Methods Nested polymerase chain reaction was used to detect HPV B19-DNA on stock bone marrow smears from 84 children with ITP. Twenty-four cases of macrocytic anemia were chosen as controls.

应用巢式聚合酶链反应技术检测84例ITP患儿存档骨髓涂片标本中HPV B19-DNA，并以24例巨幼细胞性贫血患儿为对照。

Methods 614 sputum samples were detected by centrifuging the liquored sputa and collecting and normal smear method simultaneously.

对614份痰标本同时进行夹层杯集菌离心涂片法与普通痰涂片法检测抗酸杆菌，比较2种方法的检出率。

Pretreatment Gram stain and culture of expectorated sputum should be performed only if a good-quality specimen can be obtained and quality performance measures for collection, transport, and processing of samples can be met.

如果需要做治疗之前的痰涂片革兰染色和痰培养检查，则必须要是合格的标本，同时对于标本的收集、传运、处理也要符合要求。

The suptum samples from 102 tuberculosis patients and 45 non-tuberculosis patients were detected by fluorescence quantitative pcr and acid-fast stain.

对102例培养涂阳的结核痰液标本和45例非结核感染者的痰标本，应用荧光定量pcr法、痰涂片抗酸染色法检测结核分枝杆菌。

Methods: Cervical swabs taken from 98 sex workers, 261 gynecological physical check-up clients and 599 sexual transmitted infections clinic female outpatients were cultured using commercial selective medium. Some positive cultures were further, biotyped and serotyped by PCR. Cervical or urethral swabs taken from 80 sexual transmitted infections clinic outpatients were cultured.

对98例卖淫妇女、261例妇科体检病人以及599例性病门诊女性患者的宫颈涂片进行解脲脲原体的培养，部分培养阳性的标本用PCR的方法进行Uu生物分群，对生物1群阳性的标本进一步区分血清型，并对该流行病学资料进行了统计学分析。

Methods Sputum sample of 146 patients with tuberculosis are examined by smear, Bactec detection and culture on acidic Rose Medium, with the supernatant, the middle-supernatant, the supernatant of self-sedimentation and centrifugal precipitate in sample tubes after treatment of 2%NaOH.

对146例结核病人的痰标本分别作涂片、Bactec 法检测和 2%NaOH 碱处理后，吸取标本管内上清液体、中部液体、自沉淀液体和离心沉淀法后的标本接种于酸罗氏培养基。

Through this topic, we discover with the 2%NaOH-NALC digesting fluid may in 10 minute in rapid decomposition sputamentum resisting medium, during guarantee digestion effect not because the NaOH strong function will have killed the bacterium (timing control in 20 minutes), also will not affect the magnetic pellet the function; Through the application self-restraint quota simulation clinical specimen, we have compared the acid-fast stain and the pyoktanin yellow O fluorescence dye pick out limit, the pyoktanin yellow O fluorescence dyes the dark background to after-crop the obvious green bright the bacillus tuberculosis to compare in the acid-fast stain white background the red bacillus tuberculosis to accommodate strikingly, the pyoktanin yellow O fluorescence dyes picks out limits between 0.05~0.001 Mai unit, is higher than the acid-fast stain 0.05 Mai unit, the former sensitivity is high; Moreover, concentrates the function after magnetic nano particles the specimen smear, under the mirror demonstrated that its bacillus tuberculosis quantity is higher than the specimen which obviously has not passed through concentrates.

通过本次课题，我们发现用2%NaOH-NALC消化液可在10分钟内迅速裂解痰液的粘性介质，在保证消化效果的同时不会由于NaOH过强的作用杀死细菌（时间控制在20分钟内），亦不会影响磁性颗粒的作用；通过应用自制定量模拟临床标本，我们比较了抗酸染色和金胺O荧光染色的检出限，金胺O荧光染色黑暗背景下发出明显绿色亮光的结核杆菌较抗酸染色白色背景中的红色结核杆菌更容醒目，金胺O荧光染色的检出限在0.05~0.001个麦氏单位之间，高于抗酸染色的0.05个麦氏单位，前者敏感度较高；另外，经过磁性纳米颗粒富集作用后的标本涂片，在镜下显示其结核杆菌数量明显高于未经过富集的标本。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。