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The reasons for significant error were studied by adopting TTC chromogenic method, analyzing alcohol extract of natural antimicrobic component's polarity and making sample appropriate diffusion before plate cultivated.

采用氯化三苯四氮唑对指示菌显色、分析天然产物乙醇浸膏中抗菌成分的极性、样品在平板培养前适当扩散等方法,对K-B法检测天然抗菌成分存在显著误差的原因进行了研究。

Among them, 24 rats with normal Hb value were randomly divided into 2 groups: model group in which rats received the extract of Aristolochia manshuriensis Kom by gavage, and control groupreceived tap water only by gavage.

随机选择24只Hb正常的大鼠,分为对照组及模型组,各12只,后者予关木通浸膏水溶液间断灌胃制作CAAN模型。

objective:to observe the acute toxicity and the death status of diffe rent solvent extracts of blumea ripariadc. by ig in mice.

目的:观察滇桂艾纳香药材用不同溶剂提取的浸膏灌胃给予小鼠后产生的急性毒性反应和死亡情况。

ConclusionThe extracts of Phrynium capitatum Willd flower with butyl alcohol and with water have excitatory effects on the non-pregnant or diethylstilbestrol-injected animal uterus in vitro ,which is more potent than that of Herba Leonuri fluid extract.

结论棕叶花正丁醇、水溶性组分对家兔、豚鼠、大鼠、小鼠离体子宫有明显兴奋作用。对离体子宫兴奋作用比益母草流浸膏显著。

MethodsIsolated uterus preparations of above animals were made according to the literature and its contractive effects to four different extracts of Phrynium capitatum Willd flower were observed and recorded with BL-410 experimental system for biological function; the EC50s of the extracts were determined and compared with it of Herba Leonuri fluid extract.

目的探讨棕叶花不同提取物对家兔、豚鼠、大鼠、小鼠离体子宫的兴奋作用。方法采用BL-410生物机能实验系统,观察棕叶花4种提取物对非孕或注射己烯雌酚后家兔、豚鼠、大鼠、小鼠离体子宫的兴奋作用,并用EC50与益母草流浸膏进行比较。

ConclusionThe extracts of Phrynium capitatum Willd flower with butyl alcohol and with water have excitatory effects on the non-pregnant or diethylstilbestrol-injected animal uterus in vitro ,which is more potent than that of Herba Leonuri fluid extract.

结论棕叶花正丁醇、水溶性组分对家兔、豚鼠、大鼠、小鼠离体子宫有明显兴奋作用。对离体子宫兴奋作用比益母*流浸膏显著。

MethodsIsolated uterus preparations of above animals were made according to the literature and its contractive effects to four different extracts of Phrynium capitatum Willd flower were observed and recorded with BL-410 experimental system for biological function; the EC50s of the extracts were determined and compared with it of Herba Leonuri fluid extract.

方法采用BL-410生物机能实验系统,观察棕叶花4种提取物对非孕或注射己烯雌酚后家兔、豚鼠、大鼠、小鼠离体子宫的兴奋作用,并用EC50与益母草流浸膏进行比较。

ResultsThe optimum process was : add 12 times water, soak for 3 hours, decoct 4 times and 2 hours every time.

应用 正交实验设计,以浸膏得率和黄芪甲苷含量为综合指标。

Monoaminonium Glycyrrhizinate、Diammonium Glycyrrhizinate、Monopotassium Glycyrrhizinate、Glycyrrhinic Acid、Glycyrrhetinic Acid、Dipotassium Glycyrrhizinate、Licorice Extract Block、Licorice Extract Powder、Matrine、Oxymatrine、Astragalus、Black Cohosh、Epimedium、Ginkgo Biloba、Maitake Mushroom、Red Clover、Glabridin.

甘草酸单铵、甘草酸二铵、甘草酸单钾、甘草酸二钾、甘草黄酮、甘草酸、甘草次酸、甘草浸膏、苦参碱、氧化苦参碱、植物提取物(黄芪、黑升麻、淫羊藿、银杏、灰树花、红东轴等等)、光甘草定。

Methods The orthogonal test was conducted to test the effect of the temperature of extraction, time of extraction, concentration and volume of ethanal on extracts yield and the content of hypericins and and total flavonoids from Hypericum perforatum L., and optimize the extraction technology.

方法以总金丝桃素含量、总黄酮含量及浸膏得率为指标,采用正交试验法对提取过程中提取温度、提取时间、乙醇浓度及乙醇用量4个因素进行优选研究。

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