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breast cancer cell line MCF-7 were marked killed by trametes robiniphila murr in certain concentration. The IC50 of trametes robiniphila murr in three days to MCF-7 was about 0.05mg/ml. Its cell toxin were increased while the touched time was delayed or the concentration was advanced. Trametes robiniphila murr also can advance cell toxin of adriamycin and reverse resistance of MCF-7/Adr.

用MTT 法测定不同浓度的槐耳浸膏作用不同时间下对乳腺癌细胞系MCF-7 的杀伤作用,同时测定应用槐耳浸膏时对阿霉素的增敏作用和耐药逆转作用;结论:金克在一定范围内对乳腺癌细胞系具有明显的杀伤作用,其作用3 天时,IC50 在0.05mg/ml 左右,随着药物浓度升高和作用时间的延长其杀伤作用相应的增强,对阿霉素化疗具有增敏作用和逆转耐药作用。

objectiveto predict the promoting effect of chinese medicine on proliferation of rat bone marrow mesenchymal stem cells by bioactivity fingerprint in vitro.methodsthe ointment of plastrum testudinis was extracted by petroleum ether,ether and dichloromethane in burn to gain the extracts.the effects on the proliferation of bmmscs were examined by mtt assay and flow cytometry analysis, and each sample was studied by gc-ms.

目的运用生物活性指纹图谱预测其它中药能否促进骨髓间充质干细胞(bone marrow mesenchymal stem cells,bmmscs)。方法龟版浸膏依次用石油醚、乙醚、二氯甲烷提取,得到溶剂提取物,用mtt 比色法及流式细胞仪器研究了溶剂提取物干细胞活性,采用gc-ms 技术研究每一个提取物,根据总离子流色谱图的相同保留时间和相应峰面积,获得龟版浸膏 gc-ms 生物活性指纹图谱。

Comparatively sundried flowers are more suitable to be extracted as an industrial material although the concrete yield of fresh flowers is a little more. At the same time the experiment process fully proves the selectivity of SFE-CO2. Low temperature and low pressure are suitable to extract concrete from natural flavor and fragrance;(2) The materials prepared to extract rutin are the flowers that are gotten rid of concrete by SFE-CO2. The extraction methods include the SFE-CO2 modified by alcohol, the method of hot alkali-precipitate with acid, and alkaly dissolving and acid subsiding combined with microwave or ultrasonic.

66%;产品运用GC-MS做了鉴定;虽然鲜花浸膏产率较高,但干槐花的物料状态更适合工业生产;同时实验过程充分体现了SFE-CO2的选择性,低温低压条件适合香料的SFE-CO2;(2)以超临界CO2(Supercritical CO2,简称SC-CO2)萃取过浸膏的干槐花为原料,运用夹带剂修饰的SFE-CO2、超声或微波或直接加热的碱溶酸沉法提取中药--芸香甙,相比之下,夹带剂修饰的SFE-CO2和超声辅助的碱溶酸沉法提取效果较好,芸香甙提取率分别为2。

Coefficient of Unguent of macro-reticular absorbing resin,chitosans clarification method, water-extraction and alcohol-precipitation methods were compared, and qualitative assay of Ginsenoside Rg1 ,Re, and snide measurement of Ginsenoside Rg1,Ferulic acid, and stability experiment were made.

比较大孔吸附树脂法、乙醇沉淀法和壳聚糖澄清法所得到的浸膏得率及浸膏中人参皂苷Rg1,Re的定性分析以及Rg1、阿魏酸含量测定考察。

Methods: the SMMC7721 cancer cell line were cultured in the absence of differente concentration Daphne odora Var.Marginata Jingao and assary the cell cycle by flow cytometry . MTT method was used to examine the effects of different concentration Daphne odora Var.

采用流式细胞术,观察不同浓度金边瑞香浸膏对SMMC7721细胞增殖周期的影响;采用MTT法检测不同浓度金边瑞香浸膏对SMMC7721, NKM45, A549, S180肿瘤细胞株增殖作用的影响。

II. The State practices the special-purpose control system with respect to the production, management, use and export of ephedrine (including extracts from ephedra grass and chemically synthesized, laevorotatory and dextrotatory) as well as their saline categories such as ephedrine hydrochloride, ephedrine oxalic, ephedrine sulphate and raw products of ephedrine (including ephedrine extracts, ephedrine extract powder and ephedra powder), ephedrine derivatives and prescribed preparations made with ephedrine as the raw material.

二、国家对麻黄素(含从麻黄草提取和化学合成的,包括左、右旋)及其盐类如盐酸麻黄素、草酸麻黄素、硫酸麻黄素和麻黄素粗品(含麻黄浸膏、麻黄浸膏粉、麻黄草粉)、麻黄素衍生物以及以麻黄素为原料生产的单方制剂等的生产、经营、使用、出口实行专项管理制度。

The State practices the special-purpose control system with respect to the production, management, use and export of ephedrine (including extracts from ephedra grass and chemically synthesized, laevorotatory and dextrotatory) as well as their saline categories such as ephedrine hydrochloride, ephedrine oxalic, ephedrine sulphate and raw products of ephedrine (including ephedrine extracts, ephedrine extract powder and ephedra powder), ephedrine derivatives and prescribed preparations made with ephedrine as the raw material.

二、国家对麻黄素(含从麻黄草提取和化学合成的,包括左、右旋)及其盐类如盐酸麻黄素、草酸麻黄素、硫酸麻黄素和麻黄素粗品(含麻黄浸膏、麻黄浸膏粉、麻黄草粉)、麻黄素衍生物以及以麻黄素为原料生产的单方制剂等的生产、经营、使用、出口实行专项管理制度。

The State practices the special-purpose control system with respect to the production, management, use and export of ep hedrine (including extracts from ep hedra grass and chemically synthesized, laevorotatory and dextrotatory) as well as their saline categories such as ep hedrine hydrochloride, ep hedrine oxalic, ep hedrine sulphate and raw products of ep hedrine (including ep hedrine extracts, ep hedrine extract powder and ep hedra powder), ep hedrine derivatives and prescribed pr ep arations made with ep

国家对麻黄素(含从麻黄草提取和化学合成的,包括左、右旋)及其盐类如盐酸麻黄素、草酸麻黄素、硫酸麻黄素和麻黄素粗品(含麻黄浸膏、麻黄浸膏粉、麻黄草粉)、麻黄素衍生物以及以麻黄素为原料生产的单方制剂等的生产、经营、使用、出口实行专项管理制度。

To explore the mechanism of metastatic potential inhibited with Trametes robiniophila in human hepatocellular carcinoma cells and to provide the experimental basis of Trametes robiniophila in treatment for liver cancer.

探索槐耳浸膏抑制肝癌细胞转移的机制,为槐耳浸膏在临床上治疗肝肿瘤提供理论依据。

Objective: We explore the mechanism that Trametes robiniophila inhibits proliferation of the human hepetocellular carcinoma cells and provide the experimental basis for Trametes robiniophila treating liver cancer in clinic.

目的:探索槐耳浸膏抑制肝癌细胞增殖的机理,为槐耳浸膏在临床上治疗肝肿瘤提供理论依据。

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I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像,后来回台湾,甚至去年在北京,居然也四处看见她的海报,才惊讶她的魅力之大。

Ah may dee:You are chinese living in a democratic country.

你是居住在民主国家的中国人吧。