浸泡

We used Aeromonas hydrophila which we separated from the diseased Paralichthys olivaceus to made a inactivated whole cell bacterin .We injected this vaccine in the mice and the Paralichthys olivaceus .Both the mice and the Paralichthys olivaceus gained a good protection of 80% and 70%.The fish with dipping immersion gained a good protection of 85%,and the incidence of disease was 6%.We detected the antibody level in the antiserum by agglutination reaction and indirect ELISA which seemed to be immunogenic crucially.We anatomized the mice then we found that all the purtenance of the immunzied mice are healty.All of this proved that the vaccine is effective.

本实验将所分离到的嗜水气单胞菌用终浓度为0.2%~0.5%的甲醛对菌体进行灭活，制备了灭活疫苗，并优化灭活疫苗的灭活条件；将制备的灭活疫苗分别注射小鼠和牙鲆，均取得了较为理想的效果，其中小鼠的免疫保护率达到80%，牙鲆的免疫保护率达到70%；采用渗透压差法大规模浸泡免疫牙鲆也取得了很好的效果，浸泡免疫过的牙鲆发病率仅为6%，攻毒后免疫保护率为85%；通过应用间接ELISA方法检测受免动物的抗血清，并将牙鲆的抗血清注射新西兰白兔，从而获得兔抗鱼血清，更便于检测，结果都表明动物体内产生了较高的抗体水平；剖检攻毒后的动物，发现免疫过的动物内脏健康无异样，表明该疫苗安全有效，从而为防治牙鲆腹水症开辟了一条新途径，也为进一步制备其基因工程疫苗奠定了基础。

Tow types of retting process are mainly employed for flax fibres, of which dew-retting is the commonest.

浸泡的过程的拖曳类型为胡麻纤维主要被使用，露水浸泡是最共同的。

Steeping is one of key steps of wet milling process, steeping affects starch yield and quality.

浸泡是玉米淀粉湿法加工工艺的一个重要环节，玉米浸泡的质量，直接影响到后道工序的正常生产，影响淀粉生产的得率、质量和产量。

It is necessary that searching effective methods to decide the most optimizing steeping process parameters to rise yield, reduce energy and improve quality of starch.

在整个淀粉生产过程中，浸泡工段周期最长，需要的能量较大，不适宜的浸泡操作不仅增加成本，而且影响产品质量和产量。

Methods A lamellar flap was produced in the rabbit cornea with a microkeratome. The flap interface in whole corneas and corneal beds were soaked in the rAAV –EGFP solution for 10 min, BSS solution was applied in the control group. The corneas were harvested at day 1, 3, 14, and 21 after application for the structure analysis of cornea by HE staining. Expression of the transferred gene was monitored by fluorescence stereoscope and laser confocal microscope. Results After 3d of infection with rAAV-EGFP ,slight fluorescence was detected in rabbit cornea by fluorescence stereoscope .

微型角膜刀制作兔角膜基质瓣；转染组用含rAAV－EGFP转染液浸泡角膜瓣和角膜基质床10 min，对照组用等量BSS液浸泡角膜瓣和角膜基质床10 min，不同时间点(1、3、7、14、21d)通过荧光体视镜观察角膜出现EGFP荧光的起始时间及分布情况，收集角膜，其中一半用激光共聚焦显微镜观察、照相，另一半固定后行HE染色。

Using the stolon of the Paris polyphylla as the experimental material, the extraction ratio of steroidal saponin has been studied with the orthogonal test of L16(4^5), in which the 4 factors are extraction time, ethanol concentration, ultrasonic time and the ratio for the mass of raw material drug to the volume of ethanol, and then the optimized extraction has been obtained.

以滇重楼的根茎为实验材料，重楼甾体总皂甙的提取率为评价指标，运用L16(4^5)正交试验，考察了皂甙浸出过程中的浸泡时间、乙醇浓度、超声剂量、粗粉质量与浸泡用乙醇体积之比4个因素在4个水平下的工艺过程，筛选出了最佳提取工艺。

The influence of acid rain soaking on thermostability and water vapor permeability of the leather s were investigated.

采用不同的加脂剂对猪蓝湿革进行加脂处理，并将所得到的加脂皮革在人工配制的酸雨中浸泡一定时间，考察了酸雨浸泡处理对加脂皮革透水气性能和耐干热收缩性能的影响。

Methods Cassia Seed was soaked in chromium trichloride aqueous solution of different concentration （from 0 to 3600 mg/L） at 37℃ for 12 hours.

方法用不同浓度的三氯化铬水溶液，37℃浸泡决明子过夜，收获浸泡后的决明子，作为0h时间点的样品，然后分别收获24、48、72、96h的决明子芽，用石墨炉原子吸收分光光度法测定铬含量。

The compressive strength of concretion increased gradually, then tended to unchangeableness, it was the best when the powder heated-treatment two hours at 600 3、The effect of the concreted liquid recipe, stored media and stored time, on the properties of concretion was proved up.

粉体经过600℃保温2小时处理后，其固结体的抗压强度最高。3、探明了固化液剂的配方、浸泡介质及浸泡时间等因素对固结体性能的影响规律。

Methods:(1) ADM was produced from swine skins treated with trypsin followed by Triton X-100.(2) type I collagen of mouse tail was extracted by 0.5mol/L acetoacetic acid.(3) Two kinds of ADM were got by being soaked with 0.02% glutaraldehyde for 5~10 min or being soaked with mouse type I collagen for 24h, then preserved at 4℃.

(1)应用胰蛋白酶消化-去污剂法制备ADM；(2)用0.5mol/L乙酸溶液提取制备I型鼠尾胶原；(3)用0.02%戊二醛溶液浸泡ADM5-10min，制成交联型ADM；另一部分ADM用质量分数0.25%的I型鼠尾胶原浸泡24h，制成胶原包埋型ADM，冰冻保存；(4)用酶消化法培养原代成纤维细胞，取第三代增殖期细胞，将其调整至浓度为2×105/ml的成纤维细胞悬液。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。