浸

ResultBased on directly regenerated plant of in vitro plant of Xinjiang cotton,the cotton epicotyl treated by pre-culture for 3-5d was immersed in bacteria for 15min and co-cultured for 2d.Cephalothin at 1000mg/L was used to inhibit bacteria after 50mg/L kanselection.And the combination of cytokinin with relatively high content and auxin with lowcontent was used in its directly budding culture to obtain resistant seedlings.

结果在新疆棉花离体培养植株直接再生植株基础上，经3～5d预培养处理过的棉花上胚轴，在浸菌15min共培养2d后，经50mg/Lkan筛选，用1000mg/L头孢霉素抑菌，并用相对高含量细胞分裂素和低含量生长素的组合对其进行直接出芽培养，从而获得抗性小苗。

With the competent germinating epicotyl of cotton as receptor in exogenous gene transformation, NPTII (neomycin phosphotransferase Ⅱ) gene carrying selection marker and GFP reporter gene were mediated into cotton by agrobacterium-mediated transformation. The pre-culture time, bacterial-immersing time, co-culture time and antibiotics concn., etc of using agrobacterium-mediated transformation in the transformation of competent germinating epicotyl of cotton were studied.

方法］以棉花感受态萌动的上胚轴作为外源基因转化的受体，用农杆菌介导法将携带有筛选标记NPTII基因及GFP报告基因导入棉花，研究了农杆菌介导法用于棉花感受态萌动上胚轴转化的预培养时间，浸菌时间，共培养时间及抗生素处理浓度等。

MethodWiththe competent germinating epicotyl of cotton as receptor in exogenous gene transformation,NPTIIgene carrying selection marker and GFPreporter gene were mediated into cotton by agrobacterium-mediated transformation.The pre-culture time,bacterial-i mmersing ti me,co-culture ti me and antibiotics concn.,etc of using agrobacterium-mediated transformationinthe transformation of competent germinating epicotyl of cotton were studied.

方法以棉花感受态萌动的上胚轴作为外源基因转化的受体，用农杆菌介导法将携带有筛选标记NPTII基因及GFP报告基因导入棉花，研究了农杆菌介导法用于棉花感受态萌动上胚轴转化的预培养时间，浸菌时间，共培养时间及抗生素处理浓度等。

I said,"Me too! Are you Episcopalian or Baptist?"

"我也是！圣公会还是浸信会？"

At one point recently he sounded confused as to whether he was a Baptist or an Episcopalian.

最近一次被问及宗教问题，麦凯恩似乎一度搞不清自己是浸礼会教徒还是圣公会教徒。

Are you Episcopalian or Baptist?

那你是圣公会还是浸礼会？

The water from cleaning silicon residue could be used as water-confected in refining epsom salt.

对于采用氟化物助浸所产生的氟化硅(SiF_4)气体环境问题，采用空心喷淋塔喷淋清水吸收方式处理。

After you make or buy tapers, you can cut down the dripping problem with this: 15 parts epsom salt 10 parts fruit sugar 100 parts water a smidge of plain white soap Dip and let dry, it helps some....

你买消减之后，你可以缩减这个滴水问题：泻盐15件10件100件水果糖水一浸smidge的白色肥皂，让干、它有助于：有的

The thermodynamic and equilibrium constant criterion of the silver leaching with cyanide is deduced.

推导计算了氰化物浸银过程的热力学及平衡常数判据。

The erysipelas organism is quite resistant to salting, pickling, and smoking.

猪丹毒杆菌对盐腌，酸浸，烟熏很有抵抗力。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。