海马后的

Second we use excitability medicine to provocat the nerve,s causality discharage,after 1 to 2 minutes,perfuse the brain slice with a certain concentration of puerarin to be abaissement,in result,we find that some kinds concentration(such as 1:1000) of puerarin have prominen depressant effect to the provocated discharage of the hippocampal nerves.

之后，通过在电活性较好的脑片上先进行兴奋性药物的诱发，待诱发性放电出现后1-2分钟再灌注一定浓度的葛根素进行实验观察，结果表明，一定浓度的葛根素(如1：1000)对海马神经元的诱发放电活动有显著的抑制作用。

Results:(1)NSCs form typical neurospheres under adequate concentration in vitro, which are immunoreactive to Vimentin. Typically and terminally differentiated mature neural cells could not be found without the stimulus of mitogen or only under NSCs self-regulation and self-induction;(2)NSCs derived from hippocampus maintain the character of stem cells much longer with better biological behavior; NSCs passed to the 2-3 passage are the best to graft since they have not differentiated;(3)NSCs cultured in vitro could self-regulate and differentiate into neurospheres and progenitors positively immunoreactive to specific antibodies representing neurons, astrocytes, oligodendrocytes and Schwann cells;(4)There are widespread synaptic contacts between various kinds of descendent clones and cells;(5)Neurospheres could be formed without the stimulus of mitogen when NSCs and OECs are cocultured. Many neurospheres and cells immunoreactive to Vimentin, GFAP, MAP2, 02, p75NGFR, GFAP, S-100, Synaptosis, Vimentin, Tau (Tau is only positive in cocultureof HNSCs+HOECs) could be found;(6)The supernatant fluid triturated from adult rat spinal cord stimulates NSCs to differentiate into neurons, but do not terminally differentiate;(7)Fibroblasts and O4 oligodendrocytes are not supported to grow under this culture medium.Part II: Isolation, culture and identification of rat and human olfactory ensheathing cellsOlfactory ensheathing cells/glials are the most powerful cells to enable the regeneration of axons in the central nervous system.

结果表明：①在适宜的浓度体外培养条件下，NSCs能形成典型的神经干细胞克隆球，Vimentin免疫荧光染色阳性，单靠丝裂原刺激或NSCs自我调节和分化诱导，不会产生典型的终末分化的成熟神经细胞；②海马源性的NSCs维持干细胞特性的时间更长，生物学特性更优；③传至第2～3代的NSCs尚未分化时移植最佳；④体外培养的NSCs能自我调控分化为神经元、星形胶质细胞、O2少突胶质细胞、雪旺氏细胞染色阳性克隆球和前体细胞；⑤各种子代克隆球和细胞存在广泛的突触联系；⑥NSCs与OECs联合培养时，不需丝裂原刺激即能形成克隆球，获得大量Vimentin、GFAP、MAP2、O2、p75NGFR、GFAP、S-100、Synaptosis、Vimentin、Tau(Tau只有人HNSCs+HOECs联合培养时出现阳染)染色阳性的克隆球和细胞；⑦脊髓研磨后的上清液刺激神经干细胞向神经元方向分化，但并不出现终末分化；⑧本研究培养条件不利于成纤维细胞、O4生长。

Purpose With immunohistochemical methods under the light and eletronic microscopic level, together with the computer analytic techniques , the expression changes of the synaptophysin in the hippocampus of the rats that have obtained the abilities of spatial discriminative learning and memory established by water maze during the different periods will be studied, after they stopped training. Probing into the relationship between the amenia of the spatial discriminative learning and memory with the expression changes of the synaptophys in.

目的 用免疫组化的光、电镜方法结合图像分析技术，检测经水迷宫训练获得空间辨别性学习记忆功能后的大鼠在停止训练的不同时间段其海马结构内突触素表达的变化。

The expression of Bcl-2mRNA and P53mRNA in hippocampus after 16Hz, 130dB infrasound treatment In 1 day group, Bcl-2mRNA and P53mRNA were expressed lightly in CA1-CA3 areas in hippocampus and dentate gyrus. Blood vessels dilated lightly. In 7d group, Bcl-2mRNA and P53mRNA were more expressed in areas mentioned above. Dilation of capillaries and small vessels was more obvious. Bcl-2mRNA and P53mRNA were strong positive in endothelial cells of vessels. In 14d group, Bcl-2mRNA and P53mRNA expression was obviously increased in pyramidal cells of hippocampus and granular cells of dentate gyrus, showing deep blue staining. a The endothelial cells in some vessels were also strong positive for Bcl-2mRNA and P53mRNA. Dilation of capillaries and small vessels was lessened. In 21d group, Bcl-2mRNA and P53mRNA expression was decreased in pyramidal cells. In 28d group, little change about the expression and distribution of Bcl-2mRNA and P53mRNA was observed. Lots of Bcl-2mRNA and P53mRNA were scattered in cortex of temporal lobe around hippocampus and callosal gyrus area. Dilation of capillaries and small vessels was lessened obviously. The expression of Bcl-2mRNA and P53mRNA in hippocampus after 16Hz, 90dB infrasound treatment.

次声作用后Bcl-2mRNA和P53mRNA在海马的表达，16Hz、130dB次声作用1d组：两者在海马〓区和齿状回轻度表达，海马内血管轻度扩张；7d组：两者表达在上述区域较1d增多，微血管和小血管扩张较显，血管内皮细胞内可见呈强阳性表达的Bcl-2mRNA和P53mRNA；14d组：海马锥体细胞和齿状回颗粒细胞内Bcl-2mRNA和P53mRNA阳性表达明显增多，呈深兰色，在部分血管内皮细胞内两者强阳性表达，微小血管扩张状态较7d减轻；21d组：锥体细胞胞浆内Bcl-2mRNA和P53mRNA表达减弱；28d组：上述表达及分布与21d组相比无明显改变，海马周围颞叶皮层和扣带回区域亦可见大量散在分布的Bcl-2mRNA和P53mRNA表达，血管扩张状态已明显减轻。90dB次声作用1d-7d后，较对照组无明显变化，14d-21d后轻度表达，28d后，Bcl-2mRNA和P53mRNA表达较强。

Objective To explore the expression of brain-derived neurotrophic factor in the hippocampus after ovariectomy in mice.

目的：探讨小鼠卵巢切除后的不同时期脑源性神经营养因子在海马表达的变化。

To elucidate the molecular mechanisms underlying the neural plasticity after CNS injury, the gene expression profile in the rat hippocampus following perforant path transections was explored by several methods including custom differential screening, custom cDNA array and cDNA microarray. Of 2300 cDNA clones from low-abundance rat brain cDNA bank, 6 were identified as differentially expressed genes/ESTs in the hippocampus 35 days after lesion, in which none was confirmed by Northern blot. Of 8000 cDNA elements, custom cDNA array identified 47 as differentially expressed genes/ESTs with above 3-fold changes in the hippocampus 35 days after lesion, in which 25 were up-regulated and 22 down-regulated.

中文题名去传入海马的差异表达基因筛选和Cystatin C表达副题名外文题名 Screening of the differentially expressed genes and cystatin C expression in the deafferented hippocampus 论文作者应国新导师周长福研究员学科专业神经生物学研究领域\研究方向学位级别博士学位授予单位中国科学院上海生命科学研究院学位授予日期2002 论文页码总数121页关键词海马星型胶质细胞 Cystatin C 基因表达去神经海马馆藏号BSLW /2003 /Q42 /27 为了阐明中枢神经系统损伤后神经可塑性的分子机制，本实验探索了多种方法，包括传统差异筛库、传统cDNA array和cDNA microarray，对穿通纤维切断后大鼠海马的基因表达图谱进行了研究。

After superfusing the brain slice with guanylate cyclase inhibitor,MB(3 pmol/L) for 30 min,SDR of 10 units showed significant increase as compared with control. However,MB failed to abolish the effect of L-arg on hippocampal neurons.

3给脑片灌流鸟昔酸环化酶抑制剂亚甲基蓝(3umol/L 30 min后，10个单位的平均放电频率较对照时明显增加，但亚甲基蓝不能消除L-arg对海马神经元的作用。

Behavioral changes in rats after stress were observed by open-field test,and neurogranin level of hippocampus and forebrain were determined by Western blotting.

以旷场行为任务来评定大鼠应激后的行为变化，W estern b lotting方法测定海马和前脑皮层中的NG含量。

Ito,et al reported that many surviving transplanted-NSCs migrate to reach cochlear hair cell on 28th day after transplanted into the cochlea of newborn rat,some of them took on the identical morphology of inner or outer hair cells and expressed Phalloidin.

Ito等实验表明大鼠海马来源的神经干细胞(neural stem cells，NSCs)移植入新生(P0，P2)大鼠内耳后4周，部分存活的NSCs迁移至耳蜗毛细胞表面，形态上与内、外毛细胞相似，并表达毛细胞标志物Phalloidin。

After three days of exposure to IL-2, neurite retractions, cellular swelling, vacuolations and cell rapture were evident.

另有研究显示：IL-2是中枢神经系统中抑制神经元存活的因子，IL-2作用三天后海马神经元的形态发生明显变化，出现胞体膨胀、空泡等损伤现象。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。