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Methods: The amount of Glu and glutamic decarboxylase in coriaria lactone treated hippocampus neurons were detected by high performance liquid chromatograph and liquid-scintillation radioassary.

用致痫剂马桑内酯处理培养大鼠海马神经元,用高效液相色谱仪、液体闪烁仪分别检测培养神经元内外Glu含量和神经元内谷氨酸脱羧酶活性。

Convulsive responses were scored. Slices(40 μ m thick) were cut coronally using a vibratome.

取背侧海马部位最大的脑片,进行免疫组化实验。

Aluminum could deposit in cerebral cortices and hippocampus, and induce the impairments of spatial learning and memory in rats.

铝可在大鼠脑皮质与海马蓄积,诱导其学习记忆力减退。

In PN21 rats that did not become epileptic, no signal occurred in parahippocampal cortices.

在未致癫PN21大鼠中,海马旁皮质无信号出现。

Kesslak JP,Nalcioglu O,Cotman CW,et al.Quantification of magnetic resonance scans for hippocampal and parahippocampal atrophy in Alzheimer's diseas e.

在控制杏仁核体积、年龄、性别和文化程度等因素后,所做的偏相关分析也显示:海马结构体积与MMSE、WMS、ADS和ADL无显著相关(r值分别为-0.035,-0.191,-0.07,-0.317,P值均>0.05)。

The pathological changes, the conten ts of brain water, the contents of LDH and NO in hippocampus were observed in th e rats. Results: Remarkable pathological changes were found in h ippocampus: Nissl bodies decreased or disappeared, cyton swelled.

SD大鼠侧脑室埋管,制成30%体表面积Ⅲ度烫伤模型,伤后3 d,测定脑组织含水量,海马乳酸脱氢酶和一氧化氮的含量,做病理切片,尼氏染色。

In sham-operation group,animals were decollated and their brains were taken out at 24 hours postoperatively. In all the groups, hippocampal brain injury was detected using histopathological method combined with semi-quantified RT-PCR methods for detecting caspase-3 mRNA. Adult rats aged 90 days were used in modified y maze to examine their learning and memory functions. All these three experiments were independent.

各组海马区脑损伤行组织病理学检测,半胱氨酸天冬氨酸蛋白酶-3 mRNA的表达采用半定量反转录-聚合酶反应方法进行检查,成年90 d龄的大鼠进行三等分迷宫测试其学习记忆能力,3个实验各自独立。

Mehtods Thirty male SpragueDawley rats were randomly divided into acute stress,chronic stress and control group(each n=10),acute and chronic stress models were made with forced swimming and empty bottle stimulation separately,and frequency of getting into central grille, detention time , outer grille numbers,modifying numbers and dejecture grain number were observed with openfield experimental box;expressions of neuropeptide Y and adenylcyclase in prefrontal lobe cortex, hippocampus CA3,striatum of the groups were observed using the immunohistochemistry method and correlation analyses conducted.

将30只雄性SD大鼠随机分为急性应激组、慢性应激组和正常对照组,每组10只,用强迫游泳和空瓶刺激分别制作急性和慢性应激模型,采用开野实验箱观察各组大鼠进入中央格次数、停留时间、外周格子数、修饰次数、粪便粒数;采用免疫组织化学方法检测各组大鼠神经肽Y、腺苷酸环化酶在前额叶皮质,海马CA3区,纹状体的表达水平,并进行相关分析。

Mehtods Thirty male Sprague??Dawley rats were randomly divided into acute stress,chronic stress and control group(each n=10),acute and chronic stress models were made with forced swimming and empty bottle stimulation separately,and frequency of getting into central grille, detention time , outer grille numbers,modifying numbers and dejecture grain number were observed with open??field experimental box;expressions of neuropeptide Y and adenylcyclase in prefrontal lobe cortex, hippocampus CA3,striatum of the groups were observed using the immunohistochemistry method and correlation analyses conducted.

将30只雄性SD大鼠随机分为急性应激组、慢性应激组和正常对照组,每组10只,用强迫游泳和空瓶刺激分别制作急性和慢性应激模型,采用开野实验箱观察各组大鼠进入中央格次数、停留时间、外周格子数、修饰次数、粪便粒数;采用免疫组织化学方法检测各组大鼠神经肽Y、腺苷酸环化酶在前额叶皮质,海马CA3区,纹状体的表达水平,并进行相关分析。

Results ㏕he spontaneous behavior of the bilateral common carotid arteries deligated rats decreased significantly and the expression c-fos protein of hippocampi area increased notably compared with normal groups.

结果 ①双侧颈总动脉结扎的大鼠与正常组比较水平自发行为显著减少,其海马区的C-Fos蛋白表达水平显著增高。

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