海马

Results:(1)NSCs form typical neurospheres under adequate concentration in vitro, which are immunoreactive to Vimentin. Typically and terminally differentiated mature neural cells could not be found without the stimulus of mitogen or only under NSCs self-regulation and self-induction;(2)NSCs derived from hippocampus maintain the character of stem cells much longer with better biological behavior; NSCs passed to the 2-3 passage are the best to graft since they have not differentiated;(3)NSCs cultured in vitro could self-regulate and differentiate into neurospheres and progenitors positively immunoreactive to specific antibodies representing neurons, astrocytes, oligodendrocytes and Schwann cells;(4)There are widespread synaptic contacts between various kinds of descendent clones and cells;(5)Neurospheres could be formed without the stimulus of mitogen when NSCs and OECs are cocultured. Many neurospheres and cells immunoreactive to Vimentin, GFAP, MAP2, 02, p75NGFR, GFAP, S-100, Synaptosis, Vimentin, Tau (Tau is only positive in cocultureof HNSCs+HOECs) could be found;(6)The supernatant fluid triturated from adult rat spinal cord stimulates NSCs to differentiate into neurons, but do not terminally differentiate;(7)Fibroblasts and O4 oligodendrocytes are not supported to grow under this culture medium.Part II: Isolation, culture and identification of rat and human olfactory ensheathing cellsOlfactory ensheathing cells/glials are the most powerful cells to enable the regeneration of axons in the central nervous system.

结果表明：①在适宜的浓度体外培养条件下，NSCs能形成典型的神经干细胞克隆球，Vimentin免疫荧光染色阳性，单靠丝裂原刺激或NSCs自我调节和分化诱导，不会产生典型的终末分化的成熟神经细胞；②海马源性的NSCs维持干细胞特性的时间更长，生物学特性更优；③传至第2～3代的NSCs尚未分化时移植最佳；④体外培养的NSCs能自我调控分化为神经元、星形胶质细胞、O2少突胶质细胞、雪旺氏细胞染色阳性克隆球和前体细胞；⑤各种子代克隆球和细胞存在广泛的突触联系；⑥NSCs与OECs联合培养时，不需丝裂原刺激即能形成克隆球，获得大量Vimentin、GFAP、MAP2、O2、p75NGFR、GFAP、S-100、Synaptosis、Vimentin、Tau(Tau只有人HNSCs+HOECs联合培养时出现阳染)染色阳性的克隆球和细胞；⑦脊髓研磨后的上清液刺激神经干细胞向神经元方向分化，但并不出现终末分化；⑧本研究培养条件不利于成纤维细胞、O4生长。

Objective: To observe and quantitatively analyze the microvascular architecture and senescent changes in the hippocampus of rat.

目的：观察并定量分析大鼠海马的微血管构筑及衰老变化。

Abstract］Objective:To identify senescent cultured hippocampal neurons.

目的：鉴定培养的海马神经元衰老状况。

Aim To observe differentiation of ES cells of mice in adult rat brain after transplantation into septum and hippocampus.

目的观察小鼠胚胎干细胞植人大鼠隔区和海马内后的分化情况，并对其作研究和分析。

Methods The mRNA and protein expression levels of NCAM were detected by reversed transcriptive polymerase chain reaction and Western blot after the rat hippocampal neurons were incubated with 20, 40 and 80μg/ml sodium fluoride for 24 hours in vitro.

原代培养海马细胞暴露于20、40、80μg/ml氟化钠24 h后，用RT-PCR和Western blot方法分别检测细胞内NCAM mRNA和NCAM各蛋白亚型的表达。

Hence we start to chat from the weather first, arrive Iraq war situation arrive breed of sea horse the process arrive outside the life body of the star.

于是我们先从天气聊起，到伊拉克战局到海马的繁殖过程到外星球的生命体。

Poseidon's Steed: The Story of Seahorses, from Myth to Reality.

海神波塞冬的战马：海马的故事，从神话到现实

By means of stereotaxis instrument, 1×105 BMSCs (2 μL) of passage 3 stained with Hochest33324 for 24 hours were transplanted into the left hippocampus at 24 hours, 72 hours and 7 day after induction of HIBD in the corresponding groups.

在脑立体定位仪下，24 h，72 h，7 d细胞移植组分别于造模后对应时间点，将体外培养3~5代且经Hochest33324标记24 h的鼠骨髓基质细胞2 μL脑内移植于左侧海马，约105个细胞。

Methods: Rat MSCs was isolated and purified in vitro, labelled by Hoechst33342, stereotaxis imbed into CA4 of rat hippocampus. To kill rat and recipe brain after 1,2,4 weeks to be made to frozen section, amd to observe Hoechst33342-marked cells under fluorescence microscope.

分离大鼠MSCs体外培养、纯化，Hoechst33342标记后立体定向植入大鼠海马CA4区，1、2、4周后处死大鼠取脑，制作冷冻切片；用荧光显微镜观察Hoechst3342阳性细胞。

The changes of signal intensity were synchonous. However, there was no significant change in other subcortex regions.

上述4个区域信号的抑制变化过程同步；皮层下其他区域包括海马基底节区未引出激活点。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。