海马

8 Fold in the hippocampus 10 days following perforant path injury, 32 were up-regulated and 152 down-regulated. Roughly the function categories of the target genes are: immunity reaction, environmental response, cell structure, membrane transport and protein turnover.

CustomcDNA array筛选的8000个cDNA克隆中有47个在去神经30天海马显示差异表达且表达变化在3倍以上，其中25个表达上调，22个表达下调；Northernblot实验表明cDNA array的假阳性率是较高的。cDNA microarray在大鼠中枢神经系统表达的6234种基因和EST序列中，筛选到了152个基因和EST序列在去神经10天海马差异表达，表达变化在1.5-2.8倍之间，其中表达上调的32个，下调的120个。

Of 8000 cDNA elements, custom cDNA array identified 47 as differentially expressed genes/ESTs with above 3-fold changes in the hippocampus 35 days after lesion, in which 25 were up-regulated and 22 down-regulated. Northern blot hybridization indicates that false ratio is high with the cDNA array experiment. Of 6234 rat CNS genes/ESTs, cDNA microarray identified 152 as differentially expressed cDNA clones with changes between 1.5-2.8 fold in the hippocampus 10 days following perforant path injury, 32 were up-regulated and 152 down-regulated.

Custom cDNA array 筛选的8000个cDNA 克隆中有47个在去神经30天海马显示差异表达且表达变化在3倍以上，其中25个表达上调，22个表达下调； Northern blot 实验表明cDNA array 的假阳性率是较高的。cDNA microarray在大鼠中枢神经系统表达的6234种基因和EST序列中，筛选到了152个基因和EST 序列在去神经10天海马差异表达，表达变化在1.5-2.8倍之间，其中表达上调的32个，下调的120个。

Four groups were respectively conducted in Morris water labyrinth test, which was used to dect the learning and memory of rats. Population spike in CA3 region was recorded in vivo following stimulation of lateral perforant path ,For the induction of LTP , 4 trains (50 shocks/ t rain , 500 Hz) high-frequency tetanic stimulations were applied to lateral perforant path.

MK-801组除按依赖组剂量注射海洛因外在每次注射海洛因前30分钟注射MK-801(0.3mg/kg)，四组各自进行水迷宫实验测试大鼠学习记忆；在体记录由刺激海马外侧穿通纤维(lateral perforant path，LPP)，所诱发的CA3区群体峰电位电位，并观察高频刺激引起的LTP效应；用逆转录聚合酶链反应技术半定量检测海马NMDA受体NR1、NR2A亚基mRNA的表达。

METHODS: Hippocampal slices were obtained from the Sprague-Dawley rat brain and populational signals were measured from CAl neurons of hippocampal slices using the extracellular recording technique.

细胞外记录离体大鼠海马脑片CAl区锥体细胞层群体峰电位。结果：青霉素钠500、1000和2000kU/L可剂量依赖地诱导海马脑片上CAl区神经元的兴奋。

The cell suspensions were injected into the hippocampus of rat which had received unilateral transection of fimbria-fornix before one week. 5,7,14,30,60,90,150 and 180 days after grafting, the rats were perfused and postfixed, cut section. A series of every second and third sections were used for NOS enzyme histochemistry and Nissl histochemistry.

将大鼠14～16d胚胎的基底前脑移植到单侧穹隆海马伞切断的成年大鼠海马内，动物在移植后存活5、7、14、30、60、90、150和180d分别取脑，经NADPH-d法和尼氏染色观察。

Results:The results largely conformed to previous studies and further confirmed the role of hippocampus, precuneus and posterior cingulate cortex in aMCI patients at rest,as well as that of the frontal lobe and medial temporal lobe structures in episodic memory.

在完成情节记忆任务过程中，aMCI患者的额叶、顶下小叶、海马、海马旁回和扣带回的激活较正常人增加；除顶下小叶外，其他区域局部亦表现为较正常人激活降低。

The combination of 1 H MRS and MRI can provide useful information for presurgical diagnosis of hippocampal sclerosis.

1H MRS能够早期发现海马硬化。1H MRS 和MRI互相补充有利于海马硬化的术前诊断。

Experimental study: effects of three therapies of acupuncture treatment combined with pharmacotherapy, simple acupuncture treatment and treatment of Danggui Shaoyao Powder on the dementia rats due to hippocampal CA1 lesions induced by quinolinic acid were observed.

实验研究：采用随机对照的方法，系统观察并比较了采用针药结合、单纯针刺和单纯用药疗法对QA损毁海马CA_1区所致痴呆大鼠模型的海马神经递质Glu和抗氧化指标的影响。

Methods The AD pathological model was made by hippocampal CA 1 lesions due to stereotaxic mini injection of quinolinic acid, the rat's learning and memory were observed by Y maze; the apoptosis of hippocampal cells were detected by using flow cytometry and electronic microscope.

用立体定位技术对海马CA1区微量注射喹啉酸造成AD模型；Y形迷宫检测大鼠学习记忆水平；电子显微镜、流式细胞仪检测海马细胞凋亡。

In 1-7d group, the difference between experimental group and control group was not significant. In 14-21d group, Bcl-2mRNA and P53mRNA were expressed lightly. In 28d group, the expression were comparatively strong. The results indicated that, after exposure to infrasound with some parameters, with the days elapsing, Bcl-2mRNA expression in neurons of mice hippocampus displayed certain regularity. The progressive increase suggested that infrasound could injure hippocampus, and resultantly, injure and repair neurons DNA.

据此，我们认为：当一定参数的次声作用小鼠后，随着作用天数的延长，小鼠海马内神经细胞Bcl-2mRNA表达有一定规律性，总体趋势表达增强，随次声作用次数进一步增多，21d组和28d组有产生适应的倾向，其意义在于次声作用后引起海马损伤，使神经元DNA损伤并产生修复。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。