海藻糖酶

The role of arginine to assist protein refolding was similar to that of acetone, both of them were able to combine with the denature lysozyme and improve the refolding yield.

结果表明，与自发复性收率(40%)相比，复性液中添加精氨酸、甘油、乙酰胺、丙酮、硫脲、海藻糖和甜菜碱均能有效提高变性溶菌酶的复性收率。

The result demonstrated that mannitol was the best protective substance, followed by raffinose,trehalose and melibiose, L-cysteine, xanthan gum and glycerol showed slightly protective ability.

结果表明，甘露醇对α-半乳糖苷酶的保护效果最好，其次是棉籽糖、海藻糖和蜜二糖；L-半胱氨酸、黄原胶和甘油也起到了较好的保护作用。

In this study, homologous amplification and RACE technique were used to clone the encoding gene (SpTPS1) of trehalose-6-phosphate synthase from Selaginella pulvinata, which is an endemic exrophilous plant in China. The full-length cDNA is 3223 bp long, containing an open reading frame of 2790 bp.

本研究以我国特有旱生植物垫状巻柏为材料，采用同源扩增与RACE技术相结合的方法克隆了海藻糖-6-磷酸合成酶基因SpTPS1，cDNA全长3223 bp，包括一个2790 bp的开放阅读框。

It is pointed out that the enzymatic synthesis of trehalose from starch is a promising way.

指出以淀粉为原料采用酶转化法生产海藻糖是一种较有前途的方法。

objective to investigate a method for measuring the activity of trehalose synthase.

目的研究一种测定海藻糖合成酶活性的方法。

This study has provided a new way for the enzymatic synthesis of trehalose.

本研究结合基因工程和酶工程方法，为合成海藻糖的研究提供了新的思路。

The two enzymes system α-amylase and glucoamylase were coimmobilized on celite and calcium alginate the lower in price and common use in the industry with the method of adsorption and embedding.

中文摘要：本文选取工业上较为常用、价格低廉的硅藻土和海藻酸钙为载体分别用吸附法和包埋法共固定化α-淀粉酶和糖化酶双酶体系。

Endocellular enzymes of Arthrobacter nicotinovorus D-97 showed a weak hydrolytic activity towards α-1, 4 linkage and glucose, maltose, sucrose and lactose could not be converted into trehalose.

综合采用薄层层析、高效液相电喷雾电离质谱联用(HPLC-ESI／MS)及核磁共振分析手段，在酶反应产物不必被纯化的前提下，最终证明食尼古丁节杆菌D-97对麦芽寡糖的酶反应产物中含有海藻糖。

In order to enhance the trehalose production, Y7 was used for mutation with EMS. Through primary screening and compound screening, strain L61 with high-yielding trehalose and deep neutral trehalase activity was screened.

实验对出发菌株Y7进行了EMS诱变处理，通过初筛、复筛，从中选育出一株能高产海藻糖同时具有较低海藻糖分解酶活性的菌株L61。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失