海藻糖酶

It was founded that the renaturation yield was improved in the presence of arginine, thiourea, acetone, acetamide and glycerol compared with no additives.

分析其作用机制异同点；并基于海藻糖和甜菜碱对保护蛋白质的作用，考察了海藻糖和甜菜碱在不同浓度盐酸胍和酶浓度条件下对溶菌酶辅助复性和复性动力学特性，分析了它们作用机理。

Functinal complementation test showed that trehalose-6-phosphate synthase mutant (tps1△), transformed by the open reading frame of SpTPS1 gene, restored growth on the mediam supplemented with glucose as a sole carbon source. This result indicated that trehalose-6-phosphate synthase gene (SpTPS1) of Selaginella pulvinata has biologically activity and is hopful to be applied to transgeneic improvement of plant for abiotic stress tolerance.

酵母功能互补试验证明，用SpTPS1基因开放阅读框转化的海藻糖合成酶基因突变（tps1△）酵母菌株，可恢复在以葡萄糖作为唯一碳源培养基上的生长，说明垫状巻柏海藻糖-6-磷酸合成酶基因SpTPS1具有生物活性，可能应用于植物抗逆性的转基因改良。

Enzymes of glycolysis (hexokinase,lactate dehydrogenase,aldolase) showed low activities during overwintering when more carbon flowing into treh alose synthesis.

越冬期间体内糖原磷酸化酶活性明显地增加，糖酵解有关的酶（己糖激酶、乳酸脱氢酶和醛缩酶）活性较低，以保证更多的碳源转化成海藻糖。

TPP gene plays an important role in synthesis of trehalose. The cloning of TPP gene lays a solid basis for investigating the biosynthesis of trehalose in L. bicolor.

海藻糖－6-磷酸磷酸酯酶在海藻糖合成过程中起关键作用，该基因的克隆为研究海藻糖在二色补血草中的合成奠定了基础。

The mechanism in which trehalose is produced from dextrin or starch hydrolyzate by endocellular enzymes of bacterium D-97 can be elucidated by high performance liquid chromatography with differential refraction detection basically, including the effect of the different carbon sources on the endocellular trehalose-producing enzymes in bacterium D-97 and the possibility or ability of the endocellular enzymes to produce trehalose using maltooligosaccharides of different chain lengths.

通过高效液相色谱/示差折光检测系统分析可获得细菌D-97利用糊精或淀粉水解物合成海藻糖的基本生物学信息，包括微生物培养碳源对细菌D-97胞内海藻糖合成酶系的影响以及该酶系利用不同种类或不同分子链长度的麦芽寡糖合成海藻糖的能力及作用过程。

By the partial purification of Arthrobacter nicotinovorus D-97 endocellular enzymes and the results of enzymatic reaction of enzymes of partition collection, it was found that enzymes related to trehalose synthesis were consisted of Trehalose-forming Enzyme and Trehalose-releasing Enzyme . Trehalose-forming Enzyme could convert maltooligosaccharides into non-reducing maltooligosyl trehalose by intramolecular transglycosylation, and Trehalose-releasing Enzyme could hydrolyze α-1, 4 linkage between the trehalose residue and the maltooligosaccharide residue to release trehalose and maltooligosaccharides with two glucose monomer lesser.

将食尼古丁节杆菌D-97胞内酶部分纯化并对各收集组分进行酶反应，发现其海藻糖合成酶系由海藻糖生成酶(Trehalose-forming Enzyme，TFE)和海藻糖释放酶(Trehalose-releasingEnzyme，TRE)组成，前者分子内转糖基将直链麦芽寡糖转化为没有还原性的麦芽寡糖基海藻糖，后者专一切割麦芽寡糖基海藻糖分子上麦芽寡糖基与海藻糖连接的α-1，4糖苷键，从而释放出海藻糖和减少了两个葡萄糖单体的麦芽寡糖。

To utilize trehalose,it must be hydrolyzed by trehalase for glycolysis.

海藻糖必须被海藻糖分解酶分解成葡萄糖才能用于糖酵解，以提供昆虫能量的需求。

In our research, the neutral trehalase gene in M. anisopliae was successfully cloned by PCR, RT-PCR, RACE and panhandle PCR, and submitted to GenBank, accession number are AY557613 and AY557612.

本研究利用PCR、RT-PCR、RACE、panhandle PCR 等方法成功地克隆了金龟子绿僵菌CQMa102 中性海藻糖酶基因，并登录NCBI 的GenBank，登录号为：AY557613， AY557612。

Keywords: Entomopathogenic fungus; Metarhizium anisopliae; neutral trehalase, storage stability

中文关键词：虫生真菌；金龟子绿僵菌；中性海藻糖酶；储藏稳定性

Trehalose-6-phosphate synthase, a key enzyme in trehalose synthesis pathway of plant, plays an important role in response of exrophilous rock lily and other resurrection plants to severe abiotec stress.

海藻糖-6-磷酸合成酶是植物海藻糖合成途径的关键酶，在旱生巻柏等复苏植物对逆境胁迫应答中起重要作用。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失