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Based on a lot of documents, this thesis chooses several kinds of organic or drug small molecular, using resonance light-scattering technique, UV/Vis absorption spectra method, fluorescence spectra method and 1H NMR spectra method studied the interaction mechanisms of those small molecular and biopolymer. Base on the variation of the intensity of resonance light scattering is ratio to the concentration of the protein and DNA establishes several new determination methods of protein and DNA.

本文在查阅大量文献的基础上,选择了几种有机或抗癌药物小分子,用共振光散射光谱法并结合吸光光度法、荧光光谱法以及核磁共振光谱法研究了他们与蛋白质或核酸的相互作用,根据共振光散射光谱信号的改变值与蛋白质和核酸的浓度成正比的特点,建立了几种测定纳克级蛋白质或核酸的新方法。

The net photosynthetic rate,stomatal resistance,concentration of CO2 in diachyma cell,transpiration rate,absorption rates of light by leaf,and chlorophyll contents were determined to study the physiological compensation effects of nitrogen fertilizer on winter wheat under the condition of water stress caused by various deep lysimeters.

李生秀 ,田霄鸿,李世清利用不同深度渗漏池供水差异所造成的水分胁迫,分期测定冬小麦叶片的净光合速率、气孔阻力、叶肉胞间CO2浓度、蒸腾强度、叶绿素含量和叶片吸光强度,研究养分对小麦水分胁迫的生理补偿效应。

Methods: The minimum inhibitory concentrationof Xipayiguyiy on P.gingivalis,P.intermedia and F.nucleatum was observed by agar dilution in vitro.Also,the effects of Xipayiguyinye on oral malodour challenged by cysteine was investigated on 10 volunteers in vivo.Results: It showed in this study the MIC of Xipayiguyiy for Pg and Fn was 1mg/mL,and for Pi was 0.5mg/mL.It was also found the Xipayiguyinye achieved significant effects on oral malodour challenged by cysteine.

采用琼脂稀释法,测定西帕依固龈液对牙龈卟啉单胞菌(Pg、中间普雷沃菌、具核梭杆菌的最低抑菌浓度;对10例志愿者采用自身前后对照试验,观察四种干预措施(无干预、清水、西帕依固龈液、口泰)对半胱氨酸激发性口臭的最大值水平、各时间点下降百分比及口气值的下降曲线的影响,评价西帕依固龈液防治口臭的效果。

It is possible to mark the poison degree by the spectrophotometery.

硫化氢中毒案件可通过分光光度法测定中毒者血液中硫化血红蛋白的浓度来确定中毒的程度。

By the determination of different additives influence on the crystal rules of the potassium nitrate, the primary selection to methanol, alcohol, acetone and ammonia is carried out. The results show that the methanol and ammonia have the best separation effect.

通过测定不同浓度下甲醇、乙醇、丙酮、氨四种添加剂对硝酸钾结晶的影响规律,发现甲醇和氨对硝酸钾的析出效果最好,乙醇次之,丙酮最差。

Gradients of K(superscript +), Na(superscript +) and protein along midgut were determined. The permeability of midgut segments to K(superscript +), Na(superscript +), Ca(superscript 2+), Mg(superscript 2+) and methyl blue was observed.

对中肠细胞进行了组织切片观察;测定K,Na和蛋白质沿中肠的浓度梯度,并观察了中肠对K,Na,Ca(上标 2+),Mg(上标 2+)和甲基蓝的通透性。

We employed a cell line of mouse podocytes immortalized with the simian virus 40 large tumor antigen gene, and examined the cell proliferation using colony-forming and MTT assays following treatment with various concentrations of bFGF. BMP-7, alpha-smooth muscle actin e podocytes were detected by western blot. Methyl blue staining was used for the observation of cell morphology.

利用SV40肿瘤抗原转染所获得的永生性小鼠足细胞系培养,以集落形成法和噻唑蓝MTT还原实验测定不同浓度bFGF刺激下的足细胞增殖,采用SDS-聚丙烯酰胺凝胶电泳和Western blotting 检测BMP-7、alpha-平滑肌肌动蛋白、以及波形蛋白的蛋白质水平表达,同时以甲基兰染色观察细胞形态。

METHODS HPLC-UV assay was used to analyze chiglitazar concentration in microsomes incubation system.

方法采用高效液相-紫外检测的方法测定肝微粒体孵育液中西格列他钠的浓度,用特异性抑制剂的方法分析化合物的代谢酶,用大鼠肝微粒体体外研究西格列他钠可能的代谢产物和代谢途径。

The molar dissolution enthalpies,ΔsHm, of BMIC/ZnCl2 in water with various molalities were determined by a solution-reaction isoperibol calorimeter at

在298.15K下,用具有恒温环境的溶解反应热量计测定了不同浓度离子液体BMIC/ZnCl2在水中的溶解焓,依据Pitzer方程拟合得到它们的标准摩尔溶解焓ΔsHm0和Pitzer溶解焓参数。

Compared the homogeneous enthalpic interaction coefficients of glycine in aqueous potassium chloride solutions with that in aqueous sodium chloride solutions and discussed different cations influence on the interaction of glycine in aqueous solutions. The dilution enthalpies of DMF in aqueous ethylene glycol, glycerol, glucose and sucrose solutions of various molalities have been determined using CSC4400 flow microcalorimetry at

本文选择N,N—二甲基甲酰胺为模型化合物,用流动量热法测定了298.15K下DMF在不同浓度的乙二醇、甘油、蔗糖和葡萄糖水溶液中的稀释焓,根据McMillan-Mayer理论,计算了其在不同溶剂组成下的同系焓相互作用系数,得到水溶液中多羟基化合物与酰胺的相互作用规律。

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