浓度测定

In order to select monomer of Chinese herbal polysaccharide with better immunity, four CHPs, APS, ABPS, IRPS and DOPS at six doses were added in the splenic lymphocyte of chick in vitro, and cultured 48 h. The splenic lymphocyte proliferation was determined with the MTT method.

为了筛选免疫增强作用较好的单味中药成分，将6种浓度的黄芪多糖、牛膝多糖、板蓝根多糖、山药多糖等4种中药多糖分别加入到体外培养的鸡脾脏淋巴细胞中，培养48h后，用MTT法测定淋巴细胞增殖的变化。

The determination of high molecular weight impurities which existed in cefotaxime, ceftriaxone and ceftazidime and the influences of different ion-pair reagents; pH value of the eluants and the flow rate on the chromatographic column were discussed in detail.

以建立头孢噻肟、头孢曲松、头孢他啶中高分子杂质含量的快速测定方法为目的，对不同离子对试剂、洗脱剂pH、洗脱剂类型、离子对浓度、洗脱速度等因素进行了研究。

The influences of eluent components, concentration and flow rate on the separation and determination were studied.

考查了淋洗液的组成、浓度及流速对分离和测定的影响。

The eluents were water, 10%, 20 %, 30 % and 50 % ethanol respectively and they flowed through the macroporous resin one by one with five times of the column volume. The obtained eluent of 50 % ethanol was evaporated and the content of total flavonoids was 76 % measured by HPLC.

结果 选择AB-8大孔树脂；最佳纯化工艺为上样液总黄酮质量浓度 60 mg·L-1，pH 5.0，温度 25 ℃；最佳洗脱方式是用5倍柱体积的水及体积分数分别为10 %、20 %、30 %的乙醇依次洗脱，弃去洗脱液后再用5倍柱体积的体积分数为50 %的乙醇洗脱；HPLC法测定总黄酮的含量为76 %。

With the Sand culture method, influences of different magnesium levels (0%、50%、100%、150%、200% and 300%) was explored to the wheat seedlings of different stages. Many targets such as plant height, root length, biomass, content of chlorophyll, activity of root, RGY, RGR and ratio of root to crown of the wheat were mensurated at the time of 7d, 15d and 30d of two leaves stage of wheat seedlings after their endosperms were removed at different magnesium levels.The results showed that different magnesium levels had unlike influences over the wheat seedlings.

以小麦为试材，采用砂基培养的方法，在镁浓度分别为对照组的0%、50%、100%、150%、200%和300%的营养基质上，培养剔除胚乳后的二叶期小麦幼苗7d、15d及30d，并分别测定该3个时间段小麦幼苗的株高、根长、地上部和地下部的生物量、叶绿素含量、根系活力以及相对生长速度、相对生长速率和根冠比等指标，研究不同供镁水平对不同时期小麦幼苗生长速率的影响。

The changes of biochemical properties were studied by extractability and ATPase activity measurement, SDS-PAGE analysis, and ultrastructure observation of fish meat from grass carp treated with different concentration TP and stored at 4℃.

研究方法及结果如下：1、通过测定盐溶性蛋白质的提取性、ATP酶活性、SDS-PAGE凝胶电泳、扫描电镜，分析4℃贮藏条件下的不同浓度TP处理的草鱼鱼肉蛋白质的生化特性的变化。

In order to investigate the influences of different drying methods on extraction and separation of ginsenosides, three drying processes, such as drying in the sun, drying in oven and microwave drying, were used to dry fresh ginsengs Methods: The ginsenosides of the dry ginsengs were extracted by poaching and microwave heating, and were separated by foam separation.

采用自然晾晒、烘箱乾燥和微波乾燥三种不同的方法对鲜人参进行了乾燥，观察乾燥效果；采用水煮和微波加热的方式对三种乾燥人参中的人参皂苷进行提取，进一步用泡沫分离法将各种不同皂苷成分进行分离和浓缩，测定总皂苷和单体皂苷的浓度，计算皂苷回收率。

Synoviocytes were incubated with different concentration of methotrexate and total glucosides of paeony respectively or combined.

利用mtt比色法测定不同浓度的甲氨蝶呤和白芍总苷单独或联合作用于滑膜细胞，对滑膜细胞增殖的影响。

To provide evidence for identifying adulteration in dairy drinks ,we studied the content of free glycin with glutamic acid and protein and the inter relationship in milks and dairy drinks .

为给违规添加甘氨酸掺杂造假含乳饮料的判定提供科学依据，测定了纯牛奶和含乳饮料中游离甘氨酸、谷氨酸的质量浓度和蛋白质的质量比，并对其相互比例关系进行研究。

In mouse peritoneal macrophages. Methods Mouse peritoneal macrophages were cultured in the presence of heat-killed yeast-phase PM for 24 h, and the average fluorescence intensity of TLR-2, TLR-4, and Dectin-1 in the macrophages was detected using flow cytometry. Fluorescent staining of the macrophages was performed to observe the fluorescence of TLR-2, TLR-4, and Dectin-1 with confocal microscopy. TNF-α mRNA in the cell culture supernatant was measured with real-time PCR, and TNF-α protein detected using enzyme-linked immunosorbent assay.

马尔尼菲青霉酵母相菌液与小鼠腹腔巨噬细胞共培养24h，采用流式细胞技术检测巨噬细胞TLR-2、TLR-4及Dectin-1的平均荧光强度；共聚焦显微镜观察荧光染色的受体；ELISA法测定培养液上清中TNF-α的浓度；Real time PCR检测不同时间段TNF-α的mRNA表达。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。