浓度测定

And then HepG2.2.15 cellswere cultured with different non-cellulotoxic concentration ofα-amanitin or 3TC for six days.

采用MTT法检测不同浓度α-鹅膏毒肽对体外培养的HepG2 2.2.15细胞株的毒性，然后在无毒浓度下用不同浓度α-鹅膏毒肽或拉米夫定与HepG2 2.2.15细胞共培养6天，分别在第3天和第6天收集细胞培养上清液，采用时间分辨免疫荧光法测定上清HBsAg、HBeAg浓度；收获细胞提取总RNA，行逆转录聚合酶链反应检测细胞内HBsAg和HBcAg mRNA的表达。

Methods In vitro, human gastric cancer cell line SGC-7901 in log phase was intervened by As_2O_3 (final concentrations in order:1.0,5.0,10.0μmol/L) and 5-Fu (final concentration:50mg/L) respectively alone or in combination, and incubated for 24h、 48h、 72h. Blank group is set up. The cell apoptosis and cell cycle changes were observed by flow cytometry, and the expression of apoptosis-related gene Bcl-2 was analyzed by immunocytochemical method and absolute gray scales quantitative method.

将对数生长期的人胃癌细胞株SGC-7901(终浓度为2×10~5)经低、中、高三种浓度的As_2O(终浓度分别为1.0，5.0，10.0μmol/L)和5-Fu(终浓度50mg/L)单独及联合处理，设空白对照组，分别孵育24h、48h、72h后，收取标本采用流式细胞术测定细胞凋亡率及细胞周期变化；免疫组化绝对灰度定量分析检测凋亡抑制基因Bcl-2的表达。

After the oxidative polymerization of DMP,styrene was in-situ polymerized under the initiation of dibenzoyl peroxide and dicumyl peroxide,finally thermodynamically compatible PPO/PS alloy was prepared.When styrene content was 50 wt%,for the synthesized PPO/PS alloy the yield and the weight-average molecular weight were determined to be 95%and 1.7×10~5 for PPO,93%and 2.0×10~5 for PS,respectively.The influence of the concentration of DMP and catalyst,molar ratio of N/Cu, oxygen partial pressure,NaOH concentration and temperature on the initial polymerization rate(R_0) of DMP was studied.It was found that the initial polymerization rate of DMP increased with the concentration of DMP and catalyst. The initial polymerization rate increased with the molar ratio of N/Cu at first and then decreased.

通过测定DMP氧化聚合过程中氧气吸收量随时间的变化，研究了DMP和催化剂浓度、N/Cu摩尔比、氧气分压、氢氧化钠浓度及温度对水介质中DMP氧化聚合初始速率的影响，发现DMP氧化聚合初始速率与氧气分压的0.1次方成正比；DMP氧化聚合初始速率随DMP和催化剂浓度及温度的增加而增加；随氢氧化钠浓度和N/Cu摩尔比的增加DMP氧化聚合初始速率先增加后降低。

The results manifest that the concentration of glycocholic acid has satisfactory linear correlation whith peak area, regression equation: Y= 39579X + 57823,R2=0.9984;The lowest detected concentration is 0.02μg/mL with S/N=3 ; Average recovery rate is 98.49%; RSD为0.52%～2.00%in day, RSD为0.74%～4.98%between days;The average recovery rate is 99.35%, RSD is 2.24% in the first team, and it is 100.87%, RSD is 4.73% in the second team at the stability test. When the concentration of glycocholic acid was detected in the liver, kidney and brain tissue, corresponding tissue concentration was calculated by preparing the standard curve with the glycocholic acid standard preparation in vitro, but the result can only reflect the relative chang law of the concentration of glycocholic acid in tissue but not in real tissue. The result of tissue stability experiment is consistent with that of the serum experiment.

结果表明，在0.15625～10.0μg/ml范围内血清中甘氨胆酸浓度与峰面积呈良好的线性关系，回归方程Y= 39579X + 57823，R2=0.9984；以信噪比S/N=3为标准，最低检测浓度为0.02μg/ml；平均回收率为98.49%；日内RSD为0.52%～2.00%，日间RSD为0.74%～4.98%；稳定性试验第一组的平均含量为99.35%，RSD为2.24%，第二组的平均含量为100.87%，RSD为4.73%；测定了肝、肾、脑组织中甘氨胆酸含量，采用体外甘氨胆酸标准品制备标准曲线的方法计算了相应的组织浓度，其结果仅反映组织中甘氨胆酸浓度的相对变化规律，不能体现真正的组织浓度值，但对组织样品的稳定性进行了较细致的考察，其结果和血样结果基本一致。

Two sorts of solution(alkalinized and plain bupivacaine hydrochloride) were applied to a segent of nerve,and the amplitude of the action potential was measured.②The grouping method was the same as above.After destroying the cord above the third spinal vertebrae,two sorts of bupivacaine soultion in various concentration were injected into abdominal cavity of the toad.The leg′s flexing time was measured.

①离体坐骨神经法：取蟾蜍60只，制备坐骨神经标本，分两组，实验组和对照组各30只，测定不同浓度的两组布吡卡因对离体坐骨神经动作电位幅度的影响；②在体坐骨神经丛法：取蟾蜍60只，分组同上，破坏第三脊椎以上的脊髓后，将不同浓度的两种布吡卡因注入蟾蜍的腹腔内，测定其缩腿反射消失的时间。

Rhesus macaques were induced by isoflurane for endotracheal intubation. TheMACiv. and MACmhal were determined separately by means of "up and down".The concentrations of isoflurane in inspired gas, end tidal gas and artery blood were determined at 0.5MAC and 1 .0MAC, respectively.

猕猴5只，吸入异氟醚诱导后行气管插管，当呼气末浓度低于0.2％后，采用自身前后对照，&上下法&分别测定MAC_MAC_，测定0.5、1.0MAC的吸入气、呼气未气和动脉血异氟醚浓度。

Based on this, four DBP concentrations(0.38, 0.85, 1.90, 4.25mg·L-1)were confected using the same distance of logarithmic distance, and 20 days' sub-acute toxicity test was carried out. The weights of the liver and gill of B. rerio were measured every 5 days.

在此基础上，等对数浓度差设置4个质量浓度0.38、0.85、1.90、4.25mg·L-1进行20d慢性染毒，期间每5d测定斑马鱼的体重、肝重和鳃重，同时测定斑马鱼肝脏、鳃中SOD酶和ATPase活性。

Plasma insulin,glucagon and cortisol levels were measured by radioimmunoassay,and the levels o...

用放免法测定血中胰岛素、胰高血糖素和皮质醇的浓度，用ELISA的方法测定血中IL 6和TNF α的浓度。

Plasma insulin, glucagon and cortisol levels were measured by radioimmunoassay, and the levels of TNF-α and IL-6 were detected by ELISA.

用放免法测定血中胰岛素、胰高血糖素和皮质醇的浓度，用ELISA的方法测定血中IL6和TNF-α的浓度。

Methods Glucose oxidase acted on saliva glucose,the generated hydrogen peroxide was measured by use of peroxidase with 2,4,6-tribromo-3 hydroxybenzoic acid and 4-aminoantipyrene to give a pink product that absorbed maximally at 510nm.

方法用2，4，6-三溴-3-羟基苯甲酸取代葡萄糖氧化酶测定法的苯酚，用于唾液葡萄糖的测定。并观察正常人与糖尿病患者血糖浓度和唾液葡萄糖含量之间的关系。结果186例健康人晨空腹唾液葡萄糖浓度为8.14±1.78mg/L。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

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如果有任何问题，你可以随时联系我。