浓度测定

To further validate the above results, in the second part of our research, we measured 〓 of cultured rabbit basilar artery smooth muscle cells growing on glass coverslips using ratiometric video fluorescence microscopy and the 〓-sensitive dye, fura-2, continuing with the investigation of effects of DIDS and NPPB on 5-HT induced 〓 change in these cells.

为进一步证实上述结果，本文第二部分又采用生物荧光双波长影像分析系统测定单细胞胞浆〓浓度瞬即变化技术，观察了DIDS和NPPB对5-HT引起培养的兔脑椎基底动脉平滑肌细胞胞内〓变化的影响。

Mouse genotype and C.rat genotype respectively,by RFLP and gene sequencing technique.The extracting effect of the imported commercial DNA extraction kit was the best,and the copies of COWP gene were(6.450-9.861 )×10~6 in the extraction.

Real-time PCR技术测定不同浓度NTZ和GAs作用后COWP基因拷贝数，透射电镜观察NTZ和GAs对虫体生长的影响。

Giemsa staining was used to observe the growth of C.andersoni,and transmission electron microscope was used to observe the developmental stages of C.andersoni.MTT assay was used to evaluate the toxicity of nitazoxanid and ginkgoic acids to HCT-8 cells.C.andersoni parasites growing in HCT-8 cells were treated with different concentrations of NTZ and GAs,and the copies of COWP gene were determined by Real-time PCR.

安氏隐孢子虫卵囊感染人结肠癌(HCT-8)细胞，Real-time PCR技术测定RPMI-1640培养液中分别加入不同浓度胎牛血清(fetal calf serum，FCS)、葡萄糖(glucose，GLU)、维生素C(ascorbic acid，ASC)、叶酸(folic acid，FOLIC)、泛酸钙(calcium pantothenate，CAPAN)和胰岛素(insulin，INSULIN)后虫体的增殖效果。

Methods L9(34) orthogonal test was conducted to evaluate the effects of ethanol concentration, ethanol consumption, extraction duration, and extraction time on the extraction by using the content of four kinds of sanchinosides and the content of the total flavonoids in axillary choerospondias fruit that were included in the recipe of Danxinshu capsules. HPLC and ultraviolet spectrophotometry were used in the assay of cumarin.

分别采用高效液相色谱法和紫外分光光度法测定方中三七的3种皂苷的总含量及广枣的总黄酮的含量，以两者含量的综合评分为指标，采用正交实验考察乙醇浓度、乙醇用量、提取时间、提取次数4个因素对提取效果的影响。

Methods: After LNCaP and PC3 cell lines were affected by 10, 25, 50, 75, 100 μmol/L curcumine respectively, the cell activity was assayed with MTT method at 5, 12 and 24 hours respectively; Electronic microscopy and flow cytometry were adopted to observe the morphological changes and the cell cycle of LNCaP and PC3 cell lines at 24 hours.

分别用10、25、50、75、100μmol／L浓度的姜黄素对LNCaP及PC3前列腺癌细胞株进行体外干预，5、12、24 h后四氮甲唑蓝比色法检测细胞生长活性；24 h后透射电镜观察细胞超微结构变化，流式细胞仪测定细胞凋亡及细胞周期的变化；5 h后Western印迹法检测细胞内IκBa的表达。

The concentrations of caffeine and dapsone were determined,and then their metabolic changes in vitro were studied;thus the inhibition or induction of PNS would be estimated.

检测肝药酶CYP1A2和CYP3A4的专属探针药物咖啡因、氨苯砜的原型浓度，采用原型药物减少初始速率测定法考察其体外代谢变化情况，从而评价三七总皂苷对CYP1A2、CYP3A4的诱导或抑制作用。

MethodsTo evaluate the minimal inhibitory concentration of 15 decoctions through double fold dilution method.

采用液体试管两倍稀释法测定各煎液对肺炎链球菌的最低抑菌浓度，比较各配伍组合后的药效学改变。

Methods: Ef- fects of single factor such as pH, carbon source, nitrogen source, glucose concentration and trace dements on the lipid- producing capability the strain were researched, and based on these conducted orthogonal test.

探讨了单因素如：pH、碳源、氮源、葡萄糖浓度、金属微量元素等对其产脂能力的影响，并进行了正交试验；脂肪测定方法研究，用对比分析法，并对其中的方法加以改进，采用光谱扫描以确定酵母脂肪最大吸收波长。

Having in view the lozenge arrangement of demisters and diversely shaped plates , the pressure drop and flow velocity in demisters were measured during many tests , while the demisting efficiency and the concentration of liquid droplets were calculated by the liquid balance method the effect of flow velocity and that of plate pitch on demisting efficiency as well as the influence of the number of demister stages on both the efficiency and pressure drop have been analyzed , herewith providing some referential .

针对除雾器的菱形布置方式和叶片的不同几何形状，通过大量实验测定了除雾器的压力降和速度，利用水平衡法计算了除雾效率和液滴浓度，分析了除雾器气流速度，叶片间距对除雾效率的影响及除雾器级数对除雾效率和压力降的影响，为该型除雾器的设计和制造提供了参考依据。

Morphine, penthidine and diazepam in blood were determined by high performance liquid chromatography.

利用高效液相色谱法同时分析测定了血液中吗啡、杜冷丁、安定的质量浓度。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。