浓度测定

Methods The suspension of spermatozoa was obtained from cauda epididymides using "diffusion" method. Different doses of cadmium chloride (0, 0.5, 1.0, 2.0, 4.0, 8.0, 16.0, 32.0 mg/L) were administrated to spermatozoa suspension.

方法］用扩散法收集大鼠附睾尾成熟精子制成精子悬液，分8组，CdCl2染毒浓度分别为0，0.5，1.0，2.0，4.0，8.0，16.0，32.0mg/L，每组6个平行样，染毒30min、1h、2h、4h后用CASA仪测定反映大鼠精子运动能力及运动方式的各项参数。

Each male ratwas paired with 2 female rats in proestrus when administration terminated. Femalerats were examined the next morning for the presence of sperm in vaginal smears andunderwent a cesarean section on day 13 of gestation. Then the reproductive indiceswere calculated as follows: copulation index, pregnancy index, and fertility index,which can evaluate male fertility directly. The sperm motility and morphology wereevaluated by computer-assisted sperm analysis, moreover, the caudaepididymal sperm ultramicrostructure were analyzed by transmission electronmicroscope, which can evaluate the influence on sperm maturation bydutasteride ; sperm survival rate was assessed by SYBR-14 and propidium iodidefluorescent staining; serumal dihydrotestosterone and testosterone of ratswere detected by enzyme-labeled immunoassay, which can evaluate the effecton androgenic levels; the weights of testes and epididymides, were determined byprecise electronic balance; histological examination of above tissues were evaluatedby HE staining and TEM, which can evaluate treatment on histology of reproductiveorgans by dutasteride.

给药结束后雄鼠与处于动情前期的雌鼠按2：1合笼，计算雌鼠的交配指数、受孕指数和生育指数，直接评价雄鼠生育力；采用计算机辅助精子分析系统分析大鼠附睾精子活力和形态，在此基础上合并使用透射电镜技术对各组附睾尾部精子进行透射电镜分析，评价度他雄胺对大鼠精子成熟的影响；采用SYBR-14和propidium iodide双重荧光染色计算精子存活率；采用Elisa法测定大鼠睾酮(testosterone， T)和双氢睾酮(dihydrotestosterone， DHT)血清浓度，评价度他雄胺对雄鼠雄激素水平的影响；采用精密电子天平对各组睾丸、附睾、前列腺和精囊进行称重，评价度他雄胺对大鼠生殖器官重量的影响；采用HE染色法对各组睾丸和附睾进行组织学分析，同时采用透射电镜技术对附睾上皮细胞超微结构进行分析，评价度他雄胺对大鼠主要生殖器官组织学的影响。

objectiveto study the effects of glycyrrhizin on intestinal absorption of paeoniflorin by the everted rat gut sac model.methodsused a simple rp-hplc method for the determination of paeoniflorin in the krebs fluid.

目的研究甘草酸对芍药苷大鼠肠吸收的影响。方法采用大鼠肠外翻模型研究芍药苷的肠吸收，并用反相高效液相色谱法测定大鼠肠黏膜内外两侧的芍药苷浓度。

In this study,a HPLC system equipped with an Aminex HPX-87H column was applied to determine oxalic acid in bleaching filtrates.

本研究以一个基于阴离子交换柱的离子交换色谱法作为对照方法，利用一套配备了AminexHPX-87H液相色谱柱的高效液相色谱系统测定漂白废液中的草酸浓度。

Antimicrobial susceptibilities were determined using broth microdilution MIC tests with standard and 100fold higher inocula.

采用琼脂稀释法测定头孢吡肟等5种抗生素在两种接种浓度下的MIC值。

A method for determination of high dissolved oxygenin in-situ leaching uranium process is studied.

研究了地浸采铀工艺中高浓度溶解氧的测定方法。

Methods Human luteinized granulosa cells were isolated from follicular fluid obtained during oocyte retrieval of in vitro fertilization-embryo transfer program and were cultured with M199 medium plus various concentration of leptin (0, 10, 30, 100, 300 ng/ml),human menopausal gonadotropin (hMG, 0, 0.1, 0.2, 0.5, 1, 2, 5, 10 IU/ml) and testosterone 100 μg/ml. For 2 days the media were collected for estradiol and progesterone measurements.

方法将来自体外受精-胚胎移植的卵巢黄素化颗粒细胞纯化后，在不同浓度瘦素(0、10、30、100、300 ng/ml)和人绝经期促性腺激素（hMG，0、0.1、0.2、0.5、1、2、5、10 IU/ml）单独或联合作用下进行体外培养，收集培养液，采用放射免疫方法测定颗粒细胞产生雌二醇及孕酮的量。

The plasma and nucleated cells were separated from blood for HPLC analysis of prednisone, prednisolone and cortisol.

从血样中分离出血浆和有核细胞后用HPLC法测定强的松、强的松龙和可的松的浓度。

The aim of this study was to explore the effects of overtraining on the ability to exercise and anti-fatigue.

为探讨过度运动训练对运动能力的影响，以大鼠为实验对象，以游泳作为运动方式，并给予各组不同运动量训练8周，于训练前后测定血小板聚集功能及血浆TXB2、6－keto－PGF1α浓度。

According to the bactericidal assay of quantitative vehicle, the bacteria on the ozonized vehicles and unozonized vehicles was washed, 50μl eluant was seeded on the TSA plates.

用碘滴定法测定打开臭氧发生器不同机组及不同时间时的臭氧浓度，按载体定量杀菌试验，将经臭氧作用的载体与相同环境下未经臭氧作用的载体上的细菌洗下，各取洗脱液50μl，接种于TSA平板并置厌氧箱（90％N2，10％CO2，37℃）培养24-48h后，进行活菌菌落计数。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。