浓度测定

METHODSUsing orthogonal design, total flavonoids in extract was as evaluation index, and ethanol concentration, extraction time, ratio of material to solvent and extration frequency were as inspecting factors to screened the optimum extraction technique, and total flavonoids content in extract was detected by spectrophotography.

方法］采用正交设计法以提取物中总黄酮含量为评价指标，以乙醇浓度、提取时间、固液比及提取次数为考察因素，筛选翻白草的最佳提取工艺。应用分光光度仪测定提取物中总黄酮的含量。

METHODS Ammonium sulfamate and 1 mol·L -1 hydrochloric acid were added to the benzalkonium bromide solution,which was a reference solution.

取浓度相同的两份苯扎溴铵消毒液，一份加入氨基磺酸铵和 1mol·L-1盐酸作为参比液，在 35 4 6nm测定亚硝酸钠的ΔA。

METHODS The residual concentrations of sodium formaldehyde sulfoxylate in the solutions were determined by iodimetry, the datas were fitted by linear regressions and then ...

在高精度电热恒温箱中在不断通入空气或医用纯氧的情况下，采用碘量法测定甲醛合次硫酸氢钠在水溶液中不同时刻的浓度，作出降解曲线，将测得的数据进行降解动力学方程的拟合并获得动力学参数，并计算反应速率常数。

METHODS The minimal effective bactericidal concentration of 60 strains of Gram negative bacteria resistant to QACs was detected by diluting-counting method; the phenotype to QACs by the improved phenotype screening test, the qacE-sull gene of resistance to QACs and sulphonamide compounds by PCR.

方法使用稀释计数法测定60株革兰阴性杆菌对季胺盐复合物的最低有效杀菌浓度，利用改良的表型筛选试验检测耐QACs的表型，采用聚合酶链反应试验检测耐季铵盐消毒剂-磺胺复合物基因qacE-sul1。

Methods:The minimal effective bactericidal concentration of 60 strains Gram negative bacterium resistant to QACs were detected by diluting-counting method; the phenotype to QACs by the improved phenotype screening test,the qacE-sul1 gene of resistance to QACs and Sulphonamide Compou...

使用稀释计数法测定60株革兰阴性杆菌对季胺盐复合物的最低有效杀菌浓度，使用改良的表型筛选试验检测耐QACs的表型，使用聚合酶链反应试验检测耐季铵盐消毒剂-磺胺复合物基因qacE-sul1。

MATERIALS AND METHODS: Cytotoxicity of the drugs was measured by MTT assay. The coefficient of drug interaction was used to analyze the synergistical suppressive effect of drug combination.

材料与方法：采用MTT法测定不同浓度氯化镉和顺铂对人肝癌细胞SMMC-7721增殖的抑制作用；应用两药相互作用指数进行联合作用分析。

OBJECTIVE:To de termine protein content,protein molecular weigh and purity of recombinant human granulocyte colony stimulating factor.

钟英目的：测定重组人粒细胞集落刺激因子的蛋白浓度、分子量及纯度。

Methods The cells of LLC-PK1,Hela,Caki-1,T24 and fibroblast were incubated on 96-w ell plate with addition of diffe-rent concentration of cystic fluid of ACDK and t hat of simple renal cyst. After 24 hours for reaction, MTT method was used to de termine and compare the proliferative activity of 5 cell lines.

将LLC-PK1， Hela， T24， Caki-1和成纤维细胞接种于96孔培养板中，经过24h培养，加入不同浓度的ACDK和单纯肾囊肿的囊内液作用24h，用MTT法测定培养细胞的增殖活性，比较两者之间对细胞增殖的抑制作用。

The binary complex of Al-DNAs then interacts with tetraphenylporphyrin in tetrachloromethane, forming a ternary complex of TPP-Al-DNAs.

该复合物469nm产生强烈增强的BRLS信号，并且I_正比于ctDNA和fsDNA的浓度，可用于灵敏的测定核酸，方法的检出限在皮克级。

The result is that with the influence of lanthanon, the growth rate of colonies in solid media was enhanced, the weight of thalli in liquid medium was increased, and the ability of producing cellulase of Aspergillus niger was elevated.

论文选用黑曲霉分泌产生纤维素酶，在其培养基中分别加入不同浓度的稀土离子La~(3+)、Sm~(3+)，测定其菌落的生长速度、菌丝体的生长量和发酵液的酶活力，研究稀土元素在黑曲霉生长代谢过程中的作用。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！