浓度测定

Objective To establish the determination method of cefadroxil in healthy volunteers plasma. Methods Plasma protein was precipitated by perchloric acid (10%), and paraaminobenzoic acid was used as internal standard.

目的 建立用内标法测定人血浆中头孢羟氨苄浓度的HPLC-UV方法方法用10％高氯酸直接沉淀血浆蛋白，以对氨基苯甲酸内标，测定血浆中头孢羟氨苄的浓度。

AMs that collected, pured and cultured with contine method were stimulated by LPS of different concentration(0μg/ml,0.01μg/ml,0.1μg/ml,1μg/ml,10μg/ml) for 60min or by 1μg/ml LPS for different time stage (0min,5min,15min,30min,60min,120min) to observe the dynamic change of NF-кB intranuclear level and NO production, from which the best concentration and time point of LPS stimulation were selected. In the study, all AMs were divided into 4 groups: control group, group stimulated with LPS, group interrupted by Cal C and group inhibited by PDTC. The following parameters were measured: NF-кB level in nuclear protein extraction of AMs detected with sandwich ELISA, Inter-nuclear transposition of NF-кB observed with immunocytochemistry staining, NO content in cell culture medium quantitied with nitric acid reductase assay, Morphologic change of AMs in apoptosis observed with acridine orange staining and fragmentation at genome DNA of AMs detected with apoptotic electrophoresis assay.

分离、纯化及培养大鼠肺巨噬细胞；以不同浓度的LPS(0μg/ml，0.01μg/ml，0.1μg/ml，1μg/ml，10μg/ml)和不同作用时间(0min，5min，15min，30min，60min，120min)分别刺激小室培养的细胞单层，观察NF-κB的核内浓度及NO合成量的动态变化，选择LPS的最佳用量和作用时间；然后分成四组实验，设正常对照组，LPS处理组，特异性PKC抑制剂阻断组，NF-κB抑制剂阻断组；收集培养的单层细胞及培养液；采用夹心ELISA法定量测定细胞核提取物中的NF-κB水平；免疫组化法检测NF-κB的核内移位变化；硝酸还原酶法测定细胞培养液中NO含量；吖啶橙染色观察凋亡细胞的形态学变化，凋亡电泳实验检测细胞凋亡后基因组DNA的断裂情况。

Ion exclusion chromatography was used for the determination of organic acids in both beer and white wine with common HCl solution as an eluent and tetrabutylammoniun hydroxide as a regenerant.

研究了离子排斥色谱法分离测定啤酒和白葡萄酒中有机酸；选用常见的盐酸溶液作淋洗液，以四丁基氢氧化铵为再生液，考察了淋洗液浓度、流量等因素对分离和测定的影响，对啤酒和白葡萄酒中常见有机酸在阴离子排斥色谱柱上的保留行为进行了系统的研究；通过试验确定最佳的色谱条件为盐酸浓度1。

PK values of 3-ethoxy-N-desmethyldiazepam (3-EtO-NDZ) in ethanol and acetonitrile containing various concentrations of sulfuric acid, determined by spectrophotometry and spectropolarimetry, were found to be 3.4 and 0.63 respectively. Temperature dependent racemization of enantiomeric 3-EtO-NDZ in ethanol containing various acid concentrations was studied by monitoring changes of ellipticity at 365 nm as a function of time on a spectropolarimeter.

3-乙氧基－N去甲基安定（3-EtO-NDZ；diazepam=安定）在含不同硫酸浓度的乙醇和乙腈中的pK值用光谱法和旋光光谱法作了测定，其结果分别为3.4和0.63.3-EtO-NDZ之对映异构体在含各种不同酸浓度的乙醇中与温度相关的消旋过程，用旋光光谱仪选择其圆二色谱在365nm波长处强度的改变为时间函数的方法作了测定。

To test the hypothesis, neontal rat cadiomyocytes Treat with heat shock(HS,42℃,2h) to induce the expression of HSP70,then teated with 0.5mmol/L H2O2. We first found H2O2 can reduced cadiomyocytes apoptosis and HSP70 only over-expression in the HS group, other two group only express HSP70 a little in the test of expression of HSP70.Then we tested the concentration of Ca2+, the [Ca2+ ] of CON was 192.224+6.654, the [Ca2+ ] of H2O2 group was 290.6918+8.922and the [Ca2+]of HS group was 214.2633+4.484.To further determine how HSP70 repaired the Ca2+ homestasis, we measured calcium transient of each group.

实验中应用MTT和流式细胞技术检测细胞的凋亡，发现损伤组的存活率明显低于其他2组，热休克组(Heat shock group，HS group)细胞存活率略低于正常组细胞；免疫组化结果表明只有在HS组才检测到阳性反应，即是HSP70的表达，其他2组均为弱阳性结果；应用离子成像技术对细胞内钙离子浓度进行测定，发现损伤组290.6918+8.922明显高于正常组192.224+6.654和HS组214.2633+4.484，HS组细胞内的钙离子浓度略高于正常组；为了进一步探讨HSP70对于ROS引起的心肌凋亡过程中钙离子的调控机制，又对各组的心肌细胞进行了钙瞬变的测定。

Results Eighty-four patients with pleural effusion were enrolled,among which 40 with malignant effusion,33 with infectious effusion and 11 with transudative effusion.t-PA level was higher in malig...

应用ELISA法测定组织型纤溶酶原激活物、纤溶酶原激活物抑制剂-1(PAI-1)浓度，应用乳胶增强型免疫比浊法测定D二-聚体浓度。

While other reaction conditions were setted, viscosity and yield of the product P were determined by Ubbelohde viscosity meter in different temperature;By parity of reasoning,viscosity and yield of the product P were determined in different initiator concentration、monomer concentration and monomer ratio.

固定其它反应条件不变，用乌氏粘度计测定了在不同引发剂浓度下产物P的特性黏度及产率；以此类推，测定了在不同单体浓度、单体配比条件下产物P的特性黏度及产率。

Alkalinized by natrium hydroxydatum and diluted to measure concentration of uric acid.

收集结石患者24h尿液，用浓盐酸酸化和稀释处理后，用生化分析仪测定其中钙、磷浓度，再以氢氧化钠碱化和稀释后测定尿酸浓度。

Methods: Twenty four hours urine was taken from urinary calculus patients and acidified by concentrated hydrochloric acid and diluted, concentrations of calcium and phosphonium were measured by biochemical analyzer. Alkalinized by natrium hydroxydatum and diluted to measure concentration of uric acid.

收集结石患者24h尿液，用浓盐酸酸化和稀释处理后，用生化分析仪测定其中钙、磷浓度，再以氢氧化钠碱化和稀释后测定尿酸浓度。

METHODS:①Sixty samples were randomly divided into two groups(test and contrast groups,n=30 respectively),and the sciatic nerves of the toad was isolated.Two sorts of solution(alkalinized and plain bupivacaine hydrochloride) were applied to a segent of nerve,and the amplitude of the action potential was measured.②The grouping method was the same as above.After destroying the cord above the third spinal vertebrae,two sorts of bupivacaine soultion in various concentration were injected into abdominal cavity of the toad.The leg′s flexing time was measured.

①离体坐骨神经法：取蟾蜍60只，制备坐骨神经标本，分两组，实验组和对照组各30只，测定不同浓度的两组布吡卡因对离体坐骨神经动作电位幅度的影响；②在体坐骨神经丛法：取蟾蜍60只，分组同上，破坏第三脊椎以上的脊髓后，将不同浓度的两种布吡卡因注入蟾蜍的腹腔内，测定其缩腿反射消失的时间。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。