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ConclusionThis method is rapid, sensitive and accurate which is applicable for clinical drug concentration determination and pharmic study.

日内、日间RSD在2.5%~14.3%之间。结论该测定方法快速、灵敏、准确,适用于临床治疗药物浓度监测和药动学研究的要求。

Quercetin was prepared by hydrolyzing rutin. Their antioxidant activities were tested using phosphomolybdate method, and compared with those of BHT and V.

采用磷钼酸盐法测定了不同质量浓度的粗提物、芦丁和槲皮素的抗氧化活性,并与BHT、V的抗氧化活性进行了比较。

Cultured for 48 hours, the floating cells were removed and the adherent cells were continued to culture with cytokines including IL-4 and GM-CSF. After 14 days culture, the induced DCs were observed by photics microscope and electron microscope, also co-cultured with native T cells derived from spleen, its stimulating function was detected by MTT assay.

经过光镜,透射电镜观察培养DCs的形态学特征;通过同种T细胞混合培养,采用MTT比色分析法测定不同浓度的DCs发同种T细胞增殖的能力。

Treating with different concentration of PEG 6000 in 1/2 Hoagland nutrient solution for 3 days, the physiological indexes , such as, Pn, chlorophyll fluorescence parameters, cell membrane permeability, MDA and relative water contents of Plantago asiatica L. and Viola philippcia Cay, were studied. The Pn of P. asiatica and V.

在不同浓度的PEG6000的1/2Hoagland营养液水分胁迫条件下,对处理3d的车前和紫花地丁的净光合速率、叶绿素荧光参数、细胞电解质外渗率、相对含水量和丙二醛含量等指标进行了测定和分析。

The results of the physiological and biochemical indexes in callus tissue of Poncirus trifoliata tested under calcium carbonate(CaCO3) stress indicated that except total iron content, the negative correlations among CaCO3 concentration and peroxidase activity, superoxide dismutase activity, catalase activity, active iron content, soluble protein content and callus growth were recorded.

提要测定碳酸钙(CaCO3)胁迫下枳壳愈伤组织的某些生理生化指标的结果表明:除全 Fe 含量外,CaCO3浓度与过氧化物酶活性、超氧化物歧化酶活性、过氧化氢酶活性、活性Fe含量、可溶性蛋白质含量、愈伤组织增重均呈显著或极显著负相关,与培养液pH值呈显著正相关;随着培养的进程, POD活性、活性Fe和可溶性蛋白质含量、愈伤组织增重呈降低趋势, SOD和CAT活性先升后降,含高浓度CaCO3的培养液 pH值仅在20日左右略有降低。

In the preformulation study, the HPLC method for the analysis of RSP was established firstly and RSP solubility in various vesicles such as water, 20%PEG400, series of ethanol and propylene glycol was determined.

在制剂开发的处方前研究工作中,首先建立了利培酮HPLC分析方法,测定了药物在生理盐水、20%PEG400、各系列浓度的乙醇、丙二醇等溶媒中的溶解度。

In the preformulation study, the ultraviolet spectrum and HPLC methods for the assay of α-asarone were established firstly and then the physicochemical properties, especially the octanol/water partition coefficient and the solubility in various solvent systems such as alcohol, PEG 400, IPM, octanol were examined. The prediction permeability was then compared with actual transdermal parameters obtained from the in-vitro diffusion study , and feasibility of drug transdermal absorption was then analyzed.

在制剂开发的处方前研究工作中,首先建立了α-细辛醚的紫外及HPLC分析方法,测定了药物的油水分配系数及在生理盐水、各系列浓度的乙醇、PEG400、肉豆蔻酸异丙酯、正辛醇等溶媒中溶解度;其次,确立了药物体外经皮渗透研究的方法,并考察溶媒及促渗剂(乙醇、丙二醇、肉豆蔻酸异丙酯等)对药物体外经皮渗透性能的影响,计算药物经皮渗透的各参数,根据药物的药物动力学参数,分析了药物经皮吸收的可行性。

METHOD The serum concentrations of AZP metabolite 6-Mercapto prine(6-MP) in different time were determined by reverse phase HPLC,when AZP was used alone and combined with Pred in nine rabbits.The pharmacokinetic parameters of the two groups wer statistically analysed by paired t test.

采用反相高效液相色谱法对单用硫唑嘌呤及醋酸泼尼松与硫唑嘌呤合用后,对家兔体内硫唑嘌呤的代谢产物六巯基嘌呤(6-MP)的血清药物浓度进行了测定,并对两组药物动力学参数进行配对t检验统计处理。

The in vitro release of the resultant products was studied by evaluating the marker of protocatechualdehyde at 312 nm using high efficiency liquid chromatography.

以原儿茶醛为检测标志物,高效液相色谱法测定312 nm处物质浓度,以了解体外释放情况。

Moreover,it was demonstrated that the threesubstances,E,DE and DA,increased dramatically and quickly after LPS treatment.Also,it was revealed that the changes of three substances were more quickly than thechanges of the above mentioned APPs during the same LPS treatment.Thesesuggested that the three substances existed as neurotransmitter.The presence and thechange of the CAs in amphioxus after treated with LPS indicated the evolutionaryexistence of CAs and acute phase response in the protochordate lineage.

第四,我们用高效液相色谱法(High Performance Liquid Chromatography,HPLC),探讨了LPS感染文昌鱼儿茶酚胺类神经递质的变化,优化了同时测定文昌鱼体液中NE、E和DA三种CAs的HPLC方法,并首次发现,文昌鱼体液中同时存在NE、E、和DA三种CAs物质,其中,E在文昌鱼中首次报道,这三种物质在LPS感染文昌鱼后浓度升高非常明显,与同期的APPs相比变化更为迅速,也体现了神经递质的主导作用,文昌鱼的CAs系统也具有了类似脊椎动物CAs系统的功能,可以初步判断CAs系统的进化源头由脊椎动物可推回到头索类。

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