浓度测定

In Vitro study on the impact of overexpression of RbAp46 in U937cell lineObjective To study the impact of overexpression of RbAp46 in monoblastic cell line U937. Methods Viability of transfected cells was assayed by trypan blue exclusion.Cell number was counted daily to determine the growth rate.

将处在对数生长期U937／RbAp46和U937／CMV各以2×10~5／ml浓度接种，加入TPA50ng／ml，同时并以培养基作为空白对照，培养72小时后收集细胞，流式细胞术测定细胞表面分化抗原CD11b的表达，RT-PCR分析p21mRNA的表达，流式细胞术检测细胞周期分布情况。

Detection the early stage apoptosis cells by flow cytornetry Incubating SCL-1 cells in 6-well culture medium with 1×10~6/well, after 12～48hculture with the concentration of imiquimod 0μg/mL and 150μg/mL, gathering cellsgrowing along the wall and making monocellular suspension, washing in PBS for 2times, and then add 5μL Annexin V/FITC and 10μL PI solutions, reaction for 15min avoiding light, detection the early stage apoptosis cells by flow cytometry.

流式细胞仪测定细胞早期凋亡 SCL-1细胞以1×10~6个／孔接种于6孔培养板中，使用含0μg／mL、150μg／mL咪喹莫特的培养液培养12～48h，收集贴壁生长的细胞制成单细胞悬液，PBS洗两次，分别加入5μL Annexin V／FITC和10μL浓度20μg／mL的PI溶液，混匀后室温避光孵育15min，流式细胞仪检测，结果用Cell Quest软件分析。

In the sixth chapter, according to our nations situations, a probing study on the development and application of natural mordenite was carried out at the aim to develop a new adsorbent carrier and reduce the cost of adsorbent preparation. The characters of natural mordenite were chemically changed by an acid. The effects of the acid solution concentration, temperature, mordenite size and treatment times on the physico-chemical characters of mordenite were undertaken. The adsorption equilibrium of CO on the character-changed natural mordenite was measured.

在第六章中，结合我国的实际情况，对天然丝光沸石的开发利用进行了探索性的研究，期望开发一种新的吸附剂载体，以降低吸附剂的制备成本，为此，对天然丝光沸石进行了酸化学改性，研究了酸溶液和浓度、温度和沸石粒度以及处理次数对丝光沸石物理化学性质的影响，测定了CO在改性丝光沸石上的吸附平衡。

The bioassay result that the LTsoof the FWC of B-pMT9, B-pMPX2 and B-pBA30 against 3rd-4rd instar larvae of C. quinquefasciatus were 28.53 h, 17.22 h and 7.61 h respectively, indicates that the CytlAa play a key role in the synergism with Mtx 1 for faster mosquitocidal activity.

生物测定结果表明，在对致倦库蚊蚊幼95％致死浓度下，B－pMT9、B－pMPX2和只含cyt1Aa基因的B－pBA30对3-4致倦库蚊的LT_(50)值分别为28.53h、17.22h和7.61h，说明Cyt1Aa毒素能够提高Mtx1毒素的杀蚊速度。

The concentration of myricetin on MWNT/G was determined by cyclic voltammetry.

采用循环伏安法对杨梅酮的浓度进行测定。

In this study,under laboratory conditions,using traditional microbiological culture method,select the Pb2+ stress training of Pistia stratiote and Myriophyllum spicatum L. under water culture, through the changs of root- microbial of Pistia stratiote and Myriophyllum spicatum L.,the results show that bacteria,fungi,actinomycetes have different concentrations under Pb2+ stress. It is specificity of ammonification, nitrification,andanti-Nitrosation after Pb2+ stress.

中文摘要：本研究在实验室条件下，用传统的微生物培养法，选择重金属离子Pb2+在水培条件下对大薸和穗状狐尾藻进行胁迫培养，通过测定大薸和穗状狐尾藻根系的微生物区系及与氮循环有关的四种细菌的数量变化发现：细菌、真菌、放线菌对重金属Pb2+的不同浓度的敏感性不同；重金属Pb2+胁迫对氨化、硝化、亚硝化和反硝化四类细菌的影响也表现出各自的特殊性。

Myrosinase's activity of the purified protein was detected. Moreover, myrosinase activity was activated by low concentration of ascorbic acid, which was the typical characteristic of myrosinase.

纯化的重组蛋白经测定具有芥子酶活性，而且其活性被低浓度的维生素C激活，具有芥子酶的典型特征。

Methods: The minimum concentration inhibitory to Neisseria gonorrhoeae of ceftriaxone was determined by using the agar dilution method.

采用琼脂稀释法测定头孢三嗪对淋病奈瑟菌的最小抑菌浓度(minimalinhibitoryconcentration ，MIC)。

The chromogenic reaction of cetyltrimethyl ammonium bromidewith 2,4,6-tri nitrophenol was studied.

生成的缔合物在4 2 0 nm处有最大吸收，表观摩尔吸光系数为1.6×10 4 L·m ol- 1 ·cm- 1 ，苦味酸浓度在0～12 0μg·m L- 1 范围内符合朗伯-比尔定律，此法可应用于DDNP废水中硝基酚类的测定。

METHODS: In fed rats, the effects of pentobarbital,isoflurane, sevoflurane, ketamine-medetomidine-atropine, and sufentanil-propofol-morphine on the cardiaccellular localization of hexokinase and levels of plasmaglucose and insulin were determined and compared with valuesobtained in nonanesthetized animals.

测定戊巴比妥、异氟烷、七氟烷、氯胺酮-美托咪定-阿托品和苏芬太尼-丙泊酚-吗啡麻醉下人工饲养大鼠心肌细胞己糖激酶定位和血浆葡萄糖及胰岛素浓度，并将之与未麻醉大鼠的对应参数作对比。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！