浓度测定

Methods:The tube broth methods were used to determin minimal inhibition concentrationof 13 common bacteria strains.Results:Extracts of Evodia rutaecarpaJuss.

采用试管稀释法测定吴茱萸提取物对13种临床常见病原菌的最低抑菌浓度，判定抑菌效果。

The article reviews the advanced research on screening sheepbreeds with high resistance against fasciolosis. Screening sheep breeds with high resistance against fasciolosis has been tested by measuring parameter of plasma such as packed cell volume, mean corpuscular volume, mean corpuscular hemoglobin concen tration, glutamate dehydrogenase, gamma glutamyl transferase and aspartate trans ferase and percent take of metacercariae. High resistance of Indonesian thin tail sheep against Fasciola gigantica has been confirmed. The mechanism of high res istance and the time of killing Fasciola gigantica has also been described.

本文综述了抗片形吸虫病绵羊品种的研究概况，通过红细胞压积、红细胞平均体积、红细胞平均血红蛋白浓度、血清谷氨酸脱氢酶、γ谷氨酰胺转移酶、天冬氨酸转氨酶等血液学参数，囊蚴感染成活率的测定等方法来筛选抗片形吸虫病的绵羊品种，研究证明印度尼西亚细尾绵羊对大片吸虫感染具有较高的抵抗力，并概述了其抗虫感染的机制及杀虫作用时间。

A new microwave heating extraction method replacing the conventional circumfluence is described, the flavonoids in flammulated coat onion samples has been determined with the use of spectrophotometry.

利用微波加热溶出代替常规回流提取方法，对红皮洋葱样品中黄酮类化合物进行了微波溶出—分光光度法测定，与常规方法做了对照，并讨论了微波功率、微波时间、浸提剂浓度、料液比等因素对溶出结果的影响，通过正交试验，确定了最佳工艺条件为：微波功率900W，微波提取时间5min，提取溶剂80%乙醇，料液比为1∶10。

The apoptosis was evaluated by AQ-stained fluorescin, DNA ladder by gel electrophoresis and subdiploidy by propidium iodide-stained flow cytometry. The level of lipid peroxide under KI treatment was determined by measuring the production of thiobarbituric acid reactive substances.

取健康的猪甲状腺细胞培养24h后，加入不同浓度的碘化钾，观察细胞的形态改变；琼脂糖凝胶电泳、流式细胞术分析细胞DNA的降解；MDA法测定细胞膜的脂质过氧化程度。

5G/m3. The gastightness of the stack was measured bypressure test,the half-life decay from 3000pa to 1500pa was 15 min.

熏蒸期间用PH3快速检测管测定PH3浓度。

Drug sensitive test and three-dimensional test220 strains of Pa were isolated from hospitalized patients between 2003 and 2007. K-B method was used to tested the susceptibility of 10 different antibiotics. IRPa was screened by testing the minimal inhibitory concentration of imipemem by using agar diluiion method.The susceptibility of these IRPa to the antibiotics was analysised. Three-dimensional test was used to identify the different kinds of beta lactamases from 220 strains of Pa.2.Carbarpenems hydrolytic enzyme genes and oprD2 gene were detectedamong the selected IRPa strains, PCR method was performed to detect carbapenemase genes which included GES、KPC、SPM、VIM、IMP、GIM gene and the oprD2 gene;Multiplex PCR were used to detect OXA genes and plasmid-mediated AmpC beta lactamase genes; The expression of the chromosomal AmpC beta lactamases and oprD2 genes in IRPa strains were analyzed by Real-time PCR.3.Identification and characterization of integronsIntegrase gene was detected by PCR, and the classification of integrons was performed by using restriction fragment length polymorphism.PCR was performed to detect the qacE△1-sull gene,and the gene cassetes which are located at variable region of integrons in the strains were detected to be positive.

方法1、药敏实验和三维实验收集2003～2007年临床分离的220株Pa，对这些菌株采用K-B法测定10种临床常用抗生素的药敏情况，同时采用琼脂稀释法检测亚胺培南的最低抑菌浓度(Minimal inhibitory concentration，MIC)，筛选出对亚胺培南耐药的铜绿假单胞菌，并分析其对其它抗生素的药物敏感率；采用三维实验的方法分析220株Pa产β内酰胺酶的类型。2、碳青霉烯类水解酶和oprD2蛋白的检测针对鉴定的IRPa菌株，采用普通PCR方法检测具有碳青霉烯水解作用的β内酰胺酶耐药基因(GES、KPC、SPM、VIM、IMP、GIM基因)和oprD2基因，采用多重PCR的方法检测OXA型基因和质粒携带的AmpC酶基因，用荧光定量RT-PCR方法检测oprD2蛋白基因表达情况；同时对产AmpC酶的Pa(25株，含IMP耐药和敏感株)用RT-PCR方法检测AmpC酶基因的表达量情况。3。

The diluent solution of glyoxylic acid and oxalic acid was used as the separation object.TOA (in n-octanol) was used as the solvent. The extraction characteristics of single acid and two-acid solution were studied,and the influence of pH and the concentration of complexing agent on the distribution coefficients were measured. The suitable mathematic models were proposed to describe the relationship between TOA and carboxylic acid based on assumed extraction reaction in organic phase.

摘 要：以草酸和乙醛酸的稀溶液为分离对象，采用三辛胺为萃取反应剂、正辛醇为稀释剂，研究了单溶质、双溶质有机酸的萃取分离特性，测定了溶液pH值、萃取反应剂的浓度等因素对双溶质有机酸萃取的影响，提出了萃取的化学反应发生在有机相中的假定，建立了TOA萃取草酸的数学模型。

The diluent solution of glyoxylic acid and oxalic acid was used as the separation object. TOA (in n-octanol) was used as the solvent. The extraction characteristics of single acid and two-acid solution were studied, and the influence of pH arid the concentration of complexing agent on the distribution coefficients were measured. The suitable mathematic models were proposed to describe the relationship between TOA and carboxylic acid based on assumed extraction reaction in organic phase.

以草酸和乙醛酸的稀溶液为分离对象，采用三辛胺为萃取反应剂、正辛醇为稀释剂，研究了单溶质、双溶质有机酸的萃取分离特性，测定了溶液pH值、萃取反应剂的浓度等因素对双溶质有机酸萃取的影响，提出了萃取的化学反应发生在有机相中的假定，建立了TOA萃取草酸的数学模型。

Methods Peripheral blood neutrophils were islolated from health men, and then cultured in DMEM medium containing 10% fetal calf serum for 3 h. Blank medium or gonococcal inocula was added into group A or group B. NO concentration and iNOS mRNA expression at 0, 3, 8 and 12 h were determined by cadmium reduction method and realtime quantitative fluorescent PCR, respectively.

分离正常人外周血中性粒细胞，在10%胎牛血清DMEM培养液上培养3 h，A组加入空白培养基，B组加入淋球菌菌液，随后在0、3、8、12 h分别以实时定量荧光PCR测定iNOS mRNA表达，用镉还原法检测NO浓度。

Zinc and body fat contents were determined by stomic absorption spectrometer and gravimetrically, respectively.

锌浓度及体脂肪含量分别以原子吸收光谱仪及比重法测定。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！