浓度测定

Methods Ponceau 4R was administrated orally to different groups of Drosophila melanogaster, Median lethal dose （LC50） was estimated by acute toxicity test; lifespan was tested by drosophila survival experiment; superoxide dismutase and maleic diadehyde were evaluated at different periods.

方法经口喂饲果蝇不同浓度胭脂红，通过急性毒性试验测定胭脂红半数致死浓度（median lethal dose，LC50），采用生存试验检测果蝇寿命，测定不同时间果蝇体内总超氧化物歧化酶（superoxide dismutase，SOD）活性及丙二醛（maleic dialdehyde，MDA）含量。

A method for the determination of BSA based on the quenching of BSA fluorescence on adding PS and a method for the determination of PS based on the enhancement of PS on adding BSA were developed. The experimental data were processed by the Stem-Volmer equation and Lineweaver-Burk double reciprocal equation.

在不同的pH条件下，酚藏花红与蛋白质的反应导致了蛋白质荧光猝灭及酚藏花红的荧光增强，其荧光猝灭值与酚藏花红的浓度成正比，可用于酚藏花红的分析测定；而酚藏花红荧光增强值与蛋白质浓度成正比，又可用于蛋白质的分析测定。

Theoretical analysis reveals that the reaction of the immobilized enzyme of low substrate concentration may be approximated to be of first order, while that of high substrate concentration to be of zero order.

经过对固定化酶反应动力学理论分析可知，实验条件下的低基质浓度区内测定的固定化酶反应可近似地作为一级反应，而在高基质浓度区内测定的固定化酶反应可近似地作为零级反应。

After ferment, determined the consistency of the aweto; adjust the proportion of herbs by orthogonal test, determined the amrita-chun content of mycelium and amylose content of ferment liquid.

发酵后，测定冬虫夏草菌丝体的浓度；在此提取液浓度下，用正交试验法调节这些中药用量的配比，对菌丝体中的甘露醇及发酵液中的多糖含量进行测定。

The results show that the change in the oscillating period was linearly proportional to the concentration of Benzylpenicillin Sodium over the range from 2.47mg/L- 0.0123mg/L. Using the procedure, the content of the antibiotic in a bought production is determined and the result was consistent with that by HLPC.

实验结果表明，当体系中青霉素浓度在2.47mg/L-0.0123mg/L之间时，青霉素的浓度与进样后周期的变化率存在良好的线性关系，以此作为定量分析的基础，去测定市售注射用青霉素G钠的有效含量，与高效液相色谱测定结果一致。

Rats was cannulated for in situ recirculation. UV and HPLC methods were used to determine the concentrations of phenolsulfonphthalein and scopoletin, respectively.

采用大鼠在体胃、小肠吸收实验模型，以高效液相色谱法测定灌注液中药物的浓度，紫外分光光度法测定酚红的浓度。

The standard is suitable for testing the density of benzene, toluene and dimethylbenzene of air in living district, and also suitable for density testing on benzene, toluene and dimethylbenzene of air indoor.

本标准适用于居住区大气中苯、甲苯和二甲苯浓度的测定。也适用于室内空气中苯、甲苯和二甲苯浓度的测定。

Trial of acute toxicity: The mouse were given once the drugs of The root (10g/ml)and leaf (9g/ml) of Panicled Fameflower by irrigating gastric in largest volume (0.3ml/10g).

急性毒性实验：土人参根、叶水煎液浓缩制成的最大浓度(土人参根10g／ml，土人参叶9g／ml)给予小鼠最大容积(0.3ml／10g)一次灌胃，若小鼠死亡，则测定半数致死量LD_(50；若毒性很小，测不出半数致死量：则改用一日内以小鼠能耐受的最大浓度和最大容积，灌胃3次；测定其最大给药量。

Human umbilical venous endothelial cells were cultured, and were exposed to different concerntrations of HCY with/or not with CuSO4; Cell counting of detached cells were performed with a hematocytometer; the cell survival was monitored by MTT assay and the cellular injury was evaluated by LDH release; Hoechst 33258 staining was used to observe nuclear morphological changes and the apoptosis was measured by DNA agarose gel electrophoresis ;the percentage of apoptosis was quantified by flow cytometry.

体外培养人脐静脉血管内皮细胞，用不同浓度的HCY伴／不伴生理浓度Cu~(2+)与内皮细胞作用；细胞计数板检测脱落细胞率；MTT法及乳酸脱氢酶释放率的测定来衡量细胞的存活与损伤；Hoechst 33258荧光染色观察细胞核形态变化和DNA琼脂糖凝胶电泳检测细胞凋亡，并采用流式细胞术定量测定细胞凋亡率。

Methods The inhibitory effects of psoralen on the viability of MCF-7/ADR cells were determined with MTT assay, intracellular adriamycin concentration was assayed with HPLC and the intracellular Ca2+ concentrations in different incubative duration were detected with confocal microscope.

采用MTT法测定补骨脂素对细胞增殖抑制作用，高效液相色谱法检测细胞内ADR的浓度，激光共聚焦显微镜测定细胞内不同时段的Ca2+浓度。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。