浓度测定

METHODS: The concentration of ozone that was transmitted by ozone generator at different time was determined by using iodine titrimetric method. According to the bactericidal assay of quantitative vehicle, the bacteria on the ozonized vehicles and unozonized vehicles was washed, 50μl eluant was seeded on the TSA plates. The TSA plates were put into the anaerobiotic incubator (90% N2, 10% CO2, 37℃). After 24 to 48 hours, the CFU on the plate was counted.

用碘滴定法测定打开臭氧发生器不同机组及不同时间时的臭氧浓度，按载体定量杀菌试验，将经臭氧作用的载体与相同环境下未经臭氧作用的载体上的细菌洗下，各取洗脱液50μl，接种于TSA平板并置厌氧箱(90%N2， 10%CO2， 37℃)培养24~48h后，进行活菌菌落计数。

METHODS: Involved in the three factors, temperature and times of extraction, concentration of NaOH solutions, to do the experiment by orthogonal design uniform design and the method of anthrone.

本文采用正交设计的方法，以硫酸蒽酮法测定总糖含量为评价指标，从提取温度、NaOH浓度、提取次数等因素入手，进行正交优化试验。

Methods; Rat HSCs were treated with 0.4% fetal calf serum/DMEM for 48 hours and divided into the control group and experiment groups. HSCs in the control group were treated with 10% FCS, and HSCs in the experiment groups were treated with 10% FCS and low anticoagulative activity heparin with different concentrations (8 g/L, 40 g/L, 200 g/L, 1000 g/L, 5000 g/L). MTT reduction assay was used to evaluate the effect of low anticoagulative activity heparin on the growth of rat HSCs.

肝星状细胞经体积分数为0.4%胎牛血清／DMEM培养液同步化48h后分为对照组和实验组，对照组以体积分数为10%胎牛血清处理，实验组分别以体积分数为10%胎牛血清和不同质量浓度(8 g/L， 40 g/L， 200 g/L， 1000 g/L， 5000 g/L)低抗凝肝素处理，应用MTT法测定其对肝星状细胞生长的影响。

Methods: 1. Male Sprague-Dawley rats were established renal calcium oxalate calculi model. 2. The different dose of LBP, medlar soak and apozem was given. The calcium oxalate crystallization, the renal cell apoptosis and the renal free radical level were detected. 3. The relationship of the renal cell apoptosis index, the renal free radical level and the calcium oxalate crystallization grade in the kidney tubule was determined.

1、给大鼠建立肾草酸钙结石模型；2、予以不同剂量的枸杞多糖，不同浓度的枸杞浸泡剂和煎剂灌喂，比较肾小管内草酸钙结晶的形成，测定肾小管上皮细胞的凋亡指数，自由基水平；3、分析结晶等级评分、细胞凋亡指数和自由基的相互关系，判断枸杞多糖预防肾草酸钙结石的作用机制和效果。

After arsenous acid was injected via femoral artery, arsenic concentration in serum and brain were tested by atomic absorption spectrometer.

方法用冰片对雄性Wistar大鼠灌胃给药后，经股动脉注射亚砷酸，用原子吸收光谱仪测定脑和血清中砷浓度。

Methods:Serum electrolyte, AG, standard carbonate(HCO 3 -), plasma osmotic pressure, blood urea nitrogen and serum creatinine concentration were measured in 12 control neonates and 25 asphyctic newborns by automatic biochemical assay.

应用全自动生化分析仪测定窒息组 2 5例和对照组 12例的血清电解质、阴离子隙、标准碳酸氢盐(HCO3 -)、血浆渗透压、血尿素氮和血肌酐浓度。

Allelopathic effects of vanillin and cinnamic acid, two eggplant autotoxic substances, on pepper seed germination, seedling growth and mycelium growth of Fusarium oxysporum f.sp with different concentrations were studied in laboratory and bioassay experiments by a simulative way.

运用模拟的方式，采用生物测定和室内培养的方法，研究了两种茄子自毒物质香草醛和肉桂酸各浓度对辣椒种子萌发和幼苗生长的化感效应，及其对辣椒枯萎菌菌丝生长的影响。

To investigate the relationship between autotoxicity and continuous cropping obstacle and to find a reasonable crop rotation system for Angelica sinensis (a common traditional Chinese medicinal plant). Aqueous extracts from root, stem and leaf of A. sinensis at vegetative stage were analyzed for autotoxicity and allelopathic effect on Brassica campestris, Triticum aestivum, Avena sativa which are often grown in regions where A.

为探讨当归自毒作用与连作障碍的关系并建立合理的轮作制度，利用4个浓度当归营养期根部及茎叶部水浸液对当归及其种植区常见作物油菜、小麦和燕麦的种子和幼苗进行了生物测定。

It is shown that the detection limit and linear range for benzidine are 14.4 and 30 - 400 ng/mL, respectively under the optimum experimental conditions.

在最佳实验条件下，方法的线性范围为30～400ng／mL，检出限为14.4ng／mL，对11份浓度为80ng／mL，300ng／mL的联苯胺标准溶液进行平行测定，相对标准偏差分别为2.1％和1.2％。

Isolations of the s.aureus insoluble peptidoglycan:(1) the s.aureus insoluble peptidoglycan was gained by cold, hot and hypersound affection repetat of s.aureus(ATCC25923);(2) the s.aureus insoluble peptidoglycan was gained by 10% trichloracetic acid, 5% trichloracetic acid,NH4HCO3 buffer with trypsin digestion of s.aureus(ATCC25923) in order. 2. Isolations of the s.aureus soluble peptidoglycan and quality control:(1) the s.aureus soluble peptidoglycan was released by short dose benzylpenicillin sodium and purified by sephadex G-100.(2) The quality control in the process of isolation: the soluble peptidoglycan was detected by SLP reagent kit; the LPS was detected by kinetic turbidimetric limulus test;and the protein was detected by improved LOWRY reagent kit.

金黄色葡萄球菌可溶性肽聚糖的分离提取及其质量控制(1)采用青霉素钠诱导法和葡聚糖G-100(Sephadex G-100)凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖；(2)金黄色葡萄球菌可溶性肽聚糖提取过程中的质量控制：采用SLP试剂盒对提取物定性检测，应用动态浊度法鲎试验测定提取物中内毒素含量，采用改良LOWRY试剂检测提取物中蛋白的含量；(3)采用青霉素诱导和Sephadex G-100凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖的条件：青霉素使用量、加入青霉素后孵育时间和菌液浓度等优化选择。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！