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Quantitative analysis of protein adsorption: The PS suspension containing lysozyme was subjected to ultracentrifuge (15 000 r/min) to collect clear aqueous solution. The lysozyme concentration in the clear solution was measured by spectrophotometry at 280 nm. The amount of protein adsorbed on PS surface was calculated based on the decrease in the protein concentration in the supernatant solution.

蛋白吸附定量分析:采用高速离心(15 000 r/min)法从含溶菌酶蛋白的聚苯乙烯悬浮液中分离出上清液,用紫外(280 nm)分光光度法测定经聚苯乙烯微球表面吸附后,液相蛋白质量浓度的下降值,由此推算蛋白在聚苯乙烯表面的吸附量。

Methods Beagle dog liver microsomes were prepared by using ultracentrifuge method. After incubating 0.4 μmolL^(-1) MN9202 with 1 gL^(-1) microsomes for 30 min at 37℃, the reaction was terminated by adding 0.5 mL alkalization. The RP-HPLC was used to determine the drug in the incubation mixture.

差速离心法制备Beagle犬肝微粒体酶,0.4μmolL^(-1)的MN9202与1.0gL^(-1)的肝微粒体酶在37℃水浴中孵育30min,加入0.5mL碱化液终止反应,然后采用RP-HPLC法测定孵育液中MN9202原形药物的浓度。

Methods 27 pigs were divided randomly into three groups(group C, group MODS, group HVHF).Major organs function were monitored. Erythrocyte natural immune adherence function and erythrocyte chemokine receptor were detected by rosette formation test and enzyme-linked immunosorbent assay, respectively. Plasma and ultrafiltrate concentrations of TNF α IL-1β IL-6 IL-8 IL-10 were measured.

方法27头猪随机分为对照组、MODS组及HVHF组,监测各主要脏器功能,测定红细胞CR1天然免疫粘附能力、红细胞趋化因子受体结合活性及血浆和超滤液中TNFα、IL-1β、IL-6、IL-8、IL-10浓度变化。

To establish the upper control limit for formaldehyde residue in textile matrix in the process of risk management, content of formaldehyde of textile samples collected randomly in domestic market was determined.

为获取纺织品风险管理工作中甲醛残留浓度控制上限,随机抽取国产织物样品并对其甲醛含量进行测定。

Objective To establish a HPLC method for the determination of cyclosporin A in whole blood.

目的:建立高效液相色谱法测定环孢素A全血浓度的方法。

Methods A small climate chamber was employed to control the temperature, concentration of vapor and ventilation rate. The quantity of formaldehyde released from woodiness boards was determined by 4160 Formaldehyde Analyzer of INTERSCAN.

使用小型环境测试舱控制温度、水蒸汽浓度、空气交换率,用INTERSCAN公司的4160型甲醛分析仪测定不同条件下木质板材的甲醛释放量。

A new catalytic kinetic spectrophotometric method for the indirect determination of trace sulfide has been studied. The method was based on the inhibition of sulfide on the iron catalyzed oxidation of xylenol orange by hydrogen peroxide in NH3 medium.

在氨水介质中,硫离子可阻抑Fe催化过氧化氢氧化二甲酚橙褪色,并且催化体系和阻抑体系在570nm处吸光度的差值与硫离子浓度呈线性关系,据此建立了阻抑动力学光度法间接测定痕量硫离子的新方法。

Effects of different concentrations of NaCl on the physiological mechanism of yearling seedlings of Phyllostachys pubescens was studied by measuring some physiological parameters.

以1a实生毛竹苗为材料,通过测定在不同浓度NaCl胁迫条件下的各项生理指标,研究了NaCl胁迫对其生理机制的影响。

Methods By use of random sampling, 200 5yearold and 200 12yearold children from nonferrous metal industrial areas and the same number children from nonindustrial areas were examined, which included conditions of dental caries, oral hygiene and dental fluorosis in 12 yearold children. Meanwhile, fluoride concentration in drinking water was measured. Results For the 5 yearold children, the prevalence and DMF index had no statistical difference between the industrial areas and nonindustrial areas.

选择有色金属基地儿童作为研究对象,以临近非工业区作为对照区,采用随机抽样的方法分别抽取有色金属工业区与非工业区5岁和12岁儿童各200名,共800名,调查儿童龋病患病状况、口腔卫生状况和12岁儿童氟牙症状况,并测定当地饮水氟质量浓度。

Their 95% effective concentration (EC95) value was 0.4735 μg/mL. Flumorph at the concentration of 0.5 μg/mL, can lead the inhibit rate of growth of mycelium, formulation of sporangium and germination of zoospore of Phytophthora sojae to 100%, the concentration was lower than Apron XL, Metalaxy which lead to the same inhibition rate. It indicated that Flumorph can obviously inhibit the growth of P.sojae in vitro, and effect is better than that of Apron XL and Metalaxy.

结果表明:氟吗啉对所测定的菌株4个发育阶段均有显著的抑制作用,氟吗啉对大豆疫霉菌菌丝生长EC50 值为0.1906μg/mL,EC95 值为0.4735 μg/mL;在0.5 μg/mL 浓度下对大豆疫霉菌孢子囊形成和孢子萌发抑制率达100%,低于达到相同抑制效果的金阿普隆和甲霜灵;说明该药剂在离体条件下能够有效抑制大豆疫霉菌的生长,效果好于金阿普隆和甲霜灵。

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