浓度测定

Methods: Soft agar clone forming test, cell morphology, scratch test, MIT and flow cytometry were used to examine the changes of Hep-2 cells treated by different concentration of PB in vitro. Results: There was a dose-dependent and time-dependent inhibition of the cell proliferation by PB.

应用细胞生长曲线测定法、软琼脂克隆形成试验、细胞形态观察、划痕试验、MTT法、流式细胞术等对在体外经不同浓度PB处理过的Hep-2细胞进行检测，观察细胞分化程度及运动能力的改变。

Usingthis method, the GSH content of Rana nigromaculata tadpoles under different concentrationsof Na2SeO4 and Na2SeO3 were measured. The induce of selenate to the content of GSH intadpoles makes its variation has well time-effect and dose-effect relationship. Thesephenomena demonstrate that GSH is smart to the exposure of selenate, and therefore can beused as a sensitive index to selenate pollution.

采用本法测定黑斑蛙蝌蚪在Na_2SeO_4和Na_2SeO_3不同浓度不同时间暴露染毒下机体GSH含量，硒盐对蝌蚪GSH的诱导使其变化具有较好的时间-效应及剂量-效应特征而表现应激反应提示蝌蚪GSH可对硒盐暴露作出快速灵敏的反应可作为监测硒污染的敏感生物标记。

The new method has been used for the simultaneous measurement of AFP and carcinoembryonic antigen in human sera.

解决了现有方法不能用于两种低浓度物质同时测定的问题。

OBJECTIVE: To determine the content of 5-Fu in human serum.

目的：测定5-氟尿嘧啶在人血清中的浓度。

Sex hormone deficiency is associated with the increasing risk of coronary heart disease in women.

冠心病危险的增加与性激素的不足密切相关。我们测定了660名健康妇女（14～75岁）绝经期前、后血脂和脂蛋白的浓度。

Small volume whole blood sucked using siphonage to detect blood alcohol directly and quantitatively was the obvious character of the biosensor.

利用虹吸现象吸取微量全血直接定量测定乙醇浓度是此生物传感器的特点。

Methods The selected microorganisms were cultivated at 37℃ in culture media with different concentration of sodium arsenite and counted by microscope, and arsenic content in bacteria was detected by ASA.

采用在培养基中加入不同浓度亚砷酸钠的方法研究各菌株对砷的耐受性，并在显微镜下进行细菌量计数，采用超低温捕获原子吸收分光光度仪测定菌体中砷含量。

On the base of sodium hydroxide and zinc acetate to adsorb the sulfide and the sodium borate buffer added to enhance the deposition effect,the deposition of zinc hydroxide and zinc sulfide was separated from the solution by a centrifuge ,then the soluble sulfide was determined by the method of methylence blue.

在Zn(CH3COO)2和NaOH溶液固定硫化物和硼砂缓冲液加强沉淀效果的基础上，提出用离心法将硫化物从溶液中分离出来，采用亚甲基蓝法测定水样中硫化物浓度的方法。

The SMMC-7721 hepatoma cell line of experimental groups and control groups were cultured with the addition of sodium selenite, potassium iodide or triiodothyronine (T3). The cell quantity was checked by the MTT assay , and the cell cycle was observed by flow cytometry and levels of AFP and ALB in the culture solution were determined by RIA.The SD rats were divided into positive control group, negative control group, T3 group and mixture group (the mixture of sodium selenite, KI and T3). After two weeks of the administration of sodium selenite、 KI and T3,Walker256 cells were transplanted into the abdominal cavity. The administration was kept on for another two weeks, then observed the morbility and mortality of the rats with ascites carcinoma. At the end, the ascites were for the cells counting and the cell cycles analysising.

方 法：在体外，应用细胞培养的方法，培养SMMC－7721肝癌细胞系，分为试验组和对照组，试验组加入不同浓度Se、I或T3干预，培养一段时间后，通过噻唑蓝试验、流式细胞仪，观察细胞数量及细胞周期的变化，测定细胞培养上清液中甲胎蛋白、白蛋白的水平，观察细胞分化情况；在体内，将试验用SD大鼠分成阴性对照组、阳性对照组、T3组和混合组（为Se、I和T3混合物），首先预防性用药2周，然后给试验组大鼠和阳性对照组大鼠腹腔注射Walker256细胞，制造腹水癌大鼠模型；继续用药2周，观察各组大鼠发病率及死亡率，测量不同时期大鼠血清FT3含量，通过流式细胞仪测量大鼠腹水细胞数及细胞周期变化。

The detection limits of potassium oxide, sodium oxide and zinc oxide were all less than 0.2 mg/L, and the background equivalent concentrations were all less than 6.0 mg/L.

方法测定氧化钾、氧化钠和氧化锌的检出限均小于0.2mg/L，背景等效浓度均小于6.0mg/L。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！