浓度测定

Objective: To study osteogenic capability of rabbit bone marrow stromal cells and biological characteristical identification in vitro in order to explore the appropriate methods of osteoblast culture in vitro and to select an idea source of seed cells for bone tissue engineer.

目的：研究兔骨髓基质细胞在体外培养条件下的成骨能力及生物学特性，探讨简便易行的成骨细胞体外培养方法，为组织工程选择理想的种子细胞来源。方法：取新西兰白兔骨髓液经离心后得骨髓单个核细胞，以1× 106／ml的细胞浓度进行培养，经传代培养，选择条件培养液培养(1640完全培养液中加入地塞米松10-8Smol／l，β-甘油磷酸钠10mmol／l，VitC 50μg／ml)作为骨髓基质细胞增殖和成骨的调节因子，通过倒置显微镜、HE染色、扫描电镜、碱磷酸酶(alkaline phosphatase，ALP)测定、Ⅰ型胶原免疫组化染色和四环素荧光钙染色等手段对获得的细胞进行生物学特性研究。

Then the intracellular calcium concentration of neurons was measured by laser confocal scanning microscope , represented by the mean intracellular fluorescent intensity. On the ice, the cerebral cortical tissue at the infarction margin of the section after optic chiasm was rapidly dissociated about 100mg, then made into brain homogenate in low temperature. The high performance liquid chromatography was used to determine the Glu, Asp and Gly contents in homogenized brain tissue.

视交叉平面用2％TTC染色，用图像处理软件计算出脑梗死面积占该片脑组织面积的百分比；取视交叉前平面梗死边缘区皮层组织约100mg，用酶消化法制作神经细胞悬液，用激光扫描共聚焦显微镜检测皮层神经元细胞内游离钙离子浓渡，以细胞平均的荧光强度代表细胞内钙浓度；冰上迅速分离视交叉后梗死边缘区皮层组织约100mg，于低温下制作脑组织匀浆，用高效液相色谱法测定皮层匀浆中谷氨酸、天门冬氨酸、甘氨酸的含量。

The aim of this work was to study the effect of Cu2+ on the activities of antioxidant enzyme CAT,SOD and GSH-PX in the Gill of Chlamys farreri .

将栉孔扇贝置于不同浓度的Cu2+溶液中10d，测定其鳃组织过氧化氢酶，谷胱甘肽过氧化物酶，超氧化物歧化酶的活性。

An exposure experiment was conducted to study the effects of different concentrations of plumbum iron (Pb2+) on activity of nonspecific esterase and myeloperoxidase in serum and haemocytes of Chlamys farreri .

测定了栉孔扇贝在不同浓度铅(Pb2+)胁迫下血清与血细胞中非特异性酯酶和髓过氧化物酶的活性变化。

It is based on the greatly enhancive effect of chlordiazepoxide on the chemiluminescence reaction between luminol and hydrogen peroxide in the flow system.

研究了氯氮卓在luminol-H2O2-NaOH体系中的化学发光行为，在选定的最佳实验条件下，测定了氯氮卓浓度与化学发光强度的关系。

The increase in chemiluminescence intensity was linearly proportional to the chlordiazepoxide concentration in the range from 1.11×10^(-11) to 8.35×l0^(-10) mol/L.

结果表明：在1.11×10^(-11)~8.35×10^(-10)mol/L内氯氮卓浓度与发光信号成线性关系，检出限为4.79×10^(-12)mol/L，重复测定11次的相对标准偏差为2.49%Cs=3.34×10^

The concentration of chlorine dioxide was determined by spectrophotometry according to the characteristic of decoloration of chlorophenol red with chlorine dioxide.

根据二氧化氯可氧化氯酚红使其褪色的特性，用示差分光光度法测定水中低浓度二氧化氯的含量。

Lidocaine hydrochloride reacts with phenol red or chlorophenol red to form an ion-association complex, the colours of solutions change obviously, the maximum absorption wavelength at 556 nm (PR-LDCI system) and 572 nm (CPR-LDCI system), the adding colours intensity is also proportional to the concentration of lidocaine hydrochloride, to develop spectrophotometric method of determination of lidocaine hydrochloride.

酚红、氯酚红分别与盐酸利多卡因作用，形成离子缔合物，溶液颜色发生明显改变，最大吸收波长分别为556 nm、572 nm，在对应波长处，盐酸利多卡因的浓度与溶液的增色程度呈良好线性关系，从而建立测定盐酸利多卡因的光度法。

The average recoveries of chloropicrin in vegetables and fruits at 0.02~1 mg/kg fortification level were 92.2%~111%, with relative standard deviations of

氯化苦的添加浓度在0.02~1mg/kg范围内，平均回收率为92.2%~111%，相对标准偏差为1.9%~7.2%，均在农药残留测定允许范围内。

After picric acid in water sample being chlorinated,chloropicrin is measured with ECD gas phase chromatography to determine the concentration of picric acid ,and quality is determined by retention time and quantity by external standard method.

将水样中的苦味酸氯化后，用ECD气相色谱法测定氯化苦的量，来确定苦味酸的浓度。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！