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Platelet aggregation was measured turbimetrically and plasma fibrinogen level was determined by biuret method.

采用比浊法测定兔血小板聚集率,双缩脲法测定血浆纤维蛋白原浓度。

We present a simple approach to determine the content of casein in crosslinked polyacylamide, i. e., protein molecules are complexed with copper ions by biuret reaction. The polymer containing proteins is cinerated at high temperature and then the residue is dissolved into nitric acid. Protein content is measured by determining concentration of copper ion in nitric acid solution using atomic absorption spectrum.

本文提出了一种在交联聚丙烯酰胺中测定酪蛋白的简便方法,即:利用双缩脲反应使蛋白质与铜离子络合,含蛋白质的聚合物在高温下灰化,残留物溶于硝酸,通过用原子吸收光谱测定硝酸溶液中的铜离子浓度来测量蛋白质的含量。

The steam moisture was determined indirectly by determination of caesium concentrations in the recirculated water and feedwater in steam generator by the graphite furnace atomic absorption spectrometry.

石墨炉原子吸收光谱法可以准确测定0.1μg/L至1mg/L浓度的铯,样品中可能存在的一些物质对测定没有干扰。

Local lesion assay indicated that when mixed with TMV-infected leaf juice dilution within 3 minutes, 5 disinfectants showed 100% inactivation ratio, including 30% chloride of lime 1:9 solution, 98% trisodium phosphate 1:9 solution, 7% sodium hypochlorite 1:150 solution, anonymity disinfectant used in USA 1:125 solution, and 75% alcohol solution.

摘 要:为了指导烟草漂浮育苗中剪叶工具消毒剂的使用,采用半叶法测定了9种药剂对烟草病叶汁液中TMV的钝化效果,采用叶面喷雾法测定了药剂对烟草的药害浓度与药害症状。

A sensitive spectrophotometric method for the determination of trace amounts of chlorine dioxide based on the fading reaction of chlorophenol red by chlorine dioxide has been developed in the alkaline solution.

在弱碱性条件下,二氧化氯(ClO2 )氧化氯酚红使其褪色,于 5 75nm波长处测定剩余氯酚红的吸光度,当用试剂空白参比时,吸光度的降低值与ClO2 的浓度呈直线关系,其它氧化剂在测定条件下无干扰,方法有较高的灵敏度和很好的选择性。

Methods: The total polysaccharide was extracted with distilled water from mycelia of paecilomyces cicadae. The colorimetric MTT was used to analyze the proliferative activity of PBMC, U937 and K562 which were treated with PCPS of 7 concentrations respectively in vitro. ELISA was used to detect the content of hTNF-α, hIFN-γ from supernatant of culturing PBMC that was treated with PCPS for 44 h.

用水提取蝉拟青霉菌丝体总多糖,以MTT法测定不同浓度总多糖对人外周血单个核细胞及白血病细胞株U937、K562增殖的影响,ELISA法测定多糖作用44h后,外周血单个核细胞培养上清液中hTNF-α、hIFN-γ的含量。

The fluorescence anisotropy using steady-state fluorescence polarization method was measured for the solution of β-CD and cinchonine mixed and sonicated for 1h. The anisotropy values for the solution increased with the increase of the concentrations of β-CD, which implied the existence of some complexes of large size or nanotube structure. Many type of large size complexes formed between β-CD and Cin under high concentrations in gas phase were characterized by ESI-MS in moderate experiment conditions.

将辛可宁与环糊精的混合溶液超声1个小时后,用稳态荧光偏振方法测定溶液的各向异性值,发现随着环糊精浓度的增大,溶液的荧光各向异性不断增强,这意味着溶液中形成了大尺寸即纳米级结构的环糊精与辛可宁复合物;用温和的质谱测定条件表征发现高浓度的β-CD与辛可宁在气相中可形成各种类型的大尺寸复合物。

The invention relates to an air ion static determination and an air ion static determinator, comprising air ionization collector, electric charge weigh, control circuit, computer and software system supporting measurement and data recording processing, wherein the electric charge weigh is used to isolate the air ionization collector and the computer acquisition circuit, the computer can monitor the electric quantity on the collector plate electrode.

本发明涉及一种空气离子静态测定法及空气离子静态测定仪,计算机控制的可以在线连续监测空气离子浓度的电路设计和测量方法。测试系统由空气离子采集器、电荷秤、控制电路、计算机及其支持测量和数据记录处理的软件系统构成。电荷秤通过电―光转换、光―电转换起到了把空气离子采集器与计算机采集电路隔离的作用,使采集器上的电荷不会通过采集电路放电,计算机可以监测采集器极板上的带电量。

After 4 passages in culture, the MSCs were implanted into 96 foveola with cell density of 0.08,0.1,0.2,0.4,0.6,0.8,1,2,4,6,8,10(x104/ml) respectively. Meanwhile, the MSCs were cultured in Dulbecco's modified Eagle's medium culture medium with different concentration of fetal bovine serum, the 3-4,5dimethylthiazol-2-yl-2,5-diphenyl tetrazelium bromide assay were used to determine the proliferation index of MSCs 72 hours later, the cell cycle were determine by cytometer also.

贴壁筛选法分离、纯化MSCs,取4代以后细胞,以不同密度接种于96孔培养板内,分别以不同浓度血清进行培养,72h后,采用四甲基偶氮唑盐比色法测定MSCs增殖能力并用流式细胞仪测定细胞周期。

The broth dilution method was used to determine MICs of fluoroquinolones against the QnrS-positive isolates. The accumulation of ciprofloxacin and the effect of efflux pump inhibitor, carbonyl cyanide m-chlorophenyl hydrazone for tested isolates of E.coli were measured by a fluorescenece method.

采用肉汤微量稀释法测定QnrS阳性株对氟喹诺酮类药物的MIC,荧光测定法检测盐酸环丙沙星在QnrS阳性株体内浓度的变化及能量抑制剂碳酰氰间氯苯腙对菌体内药物蓄积的影响。

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