浓度测定

OBJECTIVE:To establish the RP-HPLC method for determination of methyl hydroxybenzoate and ethyl hydroxybenzoate in oral liquid preparations METHODS:The Nova-Park C18 column(3 9mm×150mm,5μm)was used The mobile phase consisted of acetonitrile-water(25∶75)at a flow rate of 1ml/min,the detection wavelength was set at 254nm and the sensitivity was set at 0 01 AUFS RESULTS:The linear ranges of methyl hydroxybenzoate and ethyl hydroxybenzoate were 0 4 412～13 2 360μg/ml(r=0 9 999) and 0 6 672～20 0 160μg/m...

目的 ：建立内服液体制剂中羟苯甲酯和羟苯乙酯的反相高效液相色谱测定方法。方法：色谱柱：Nova -ParkC18 柱(3 9mm×150mm ，5μm)；流动相：乙腈-水(25∶75)；流速：1ml/min ；检测波长：254nm ；灵敏度：0 01AUFS。结果：羟苯甲酯浓度在0 4412～13 2360μg/ml范围内线性关系良好(r=0 9999)；羟苯乙酯浓度在0 6672～20 0160μg/ml范围内线性关系良好(r=0 9998)。结论：该方法简便、快速、准确，可用于医院制剂中防腐剂的检测。

The EI of different concentrations (7%vol, 8%vol, 9%vol) were iv. infused. The minimum induction dose, the dose of Eisol%, MAC, the cardiac anesthetisia index and the maximal tolerant concentration of end-tidal isoflurane were determined. The animals were autopsied after dead and the main organs were histo-pathologically diagnosed as well.

分别静脉注射7％vol、8％vol、9％volEI测定EI静脉给药的最小诱导剂量、使呼气末浓度达1％的累加用药量DE_(iso1％，MAC以及心脏麻醉指数和最大耐受末呼气浓度，动物死亡后即刻取重要脏器送病理学检查。

The factors, such as temperature, dissolved oxygen concentration, autotrophs concentration, initiative ammonia concentration and organic substance had remarkable influences on autotrophs maximum growth rate.

温度、溶解氧、接种污泥浓度、起始氨氮浓度以及污水中的有机物均会对自养细菌最大增长速率的测定产生重要影响。

The results of our bioassay study showed that the mortality rate of the treated larvae depends on the concentration of...

生物测定结果表明，幼虫死亡率依多角体浓度和虫龄而变化，在25℃条件下，3龄幼虫的致死中浓度为1×10~(5.3)多角体/毫升。

Department of Chemical Pharmacology, Capital University of Medical Science, Beijing 100054, ChinaAbstract: A reversedphase high performance liquid chromatographic method was developed for the simultaneous quantitative determination of caffeine, dapsone and chlorzoxazone. The operation was carried out on a C18 column (250 mmX4.6 mm i.d., 5.0 μm) with the mobile phase of acetonitrilephosphate buffer including 0.02 mol/L KH2PO4 and 0.02 mol/L (C2H53N, pH 6.5(25:75 in volume ratio).

用反相高效液相色谱同时测定血清中咖啡因、氨苯砜、氯唑沙宗探针药物的质量浓度，以乙腈磷酸盐缓冲体系(含 0.02 mol/L的磷酸二氢钾和0.02 mol/L的三乙胺，pH 6.5)(体积比为25：75)为流动相，以安替比林为内标，经C18柱(250 mmX4.6 mm i.d。， 5.0 μm)分离，紫外检测器检测，使3种探针药物得到较好的分离，并且在有效血药浓度范围内线性良好。

The -300V and -500V electrets were applied on 3T3 cells for 24 and 48h, respectively, then the surface charge density on 3T3 cells and the intracellular free Ca2+ concentration and the number of apoptosis were measured by means of cell electrophoresis and flow cytometry method, respectively.

利用Fluo—3/AM Ca2+指示剂，采用流式细胞术测定3T3细胞内钙离子浓度的变化和3T3细胞的凋亡量，探讨驻极体诱导3T3细胞凋亡与细胞表面电荷密度和游离钙浓度变化之间的关系。

The monoclonal antibody was produced by the method of inoculating BALB/c mice intraperitoneally with the hybridoma cells, and purified with protein A-Sepharose 4B affinity chromatography.3 Establishing of ELISA protocol to detect hBLySTwo kind of different ELISA protocols were tried to study the experiment condition of detecting hBLyS level. Streptavidin was used to indirect coating in protocal 1, but biotin-avidin system was used to enlarge the final reaction signal in protocal 2. The optimal reaction condition was chosen by the experiments. The sensitivity of protocal 1 was poor, but the linear relation of protocal 2 standard curve was excellent when the concentration of hBLyS was between 3.2~400 ng/mL. So a protocal was established which sensitivity can reach to nanogram.

建立检测hBLyS蛋白含量的ELISA方法选用2种ELISA方案对hBLyS蛋白含量的测定方法进行研究，方案一用链霉亲和素做间接包被，方案二用生物素-亲和素系统做终反应放大；优化选择2种检测方法的反应条件；方法一的敏感度较低：方法二在hBLyS蛋白浓度为3.2～400 ng/mL范围内，标准曲线的线性关系良好；从而建立了一种可检测hBLyS蛋白浓度为ng级水平的ELISA方法。

objective to study the effects of rabdosiae serrae compound preparation on staphylococcus aureus biofilm.methods minimal inhibitory coh centration were tested by doubling dilution,and simulate s.aureus biofilm in vitro be improved brown plate method.the effects of r.serrae compound preparation in different mic on s.aureus biofilm were determined and confirmed by scanning electronic microscope.single layer cells of human floss film were used to test the toxic effects of the compoud preparation.results the mic of s.serrae compound to s.aureus was 128?

目的 研究溪黄草复方对金黄色葡萄球菌生物膜的影响。方法采用试管二倍稀释法测定药物的最低抑菌浓度，用brown平板改良法体外复制金黄色葡萄球菌生物膜模型，并检测溪黄草复方的不同mic浓度对金黄色葡萄球菌生物膜的影响，扫描电镜确认。采用人绒毛膜单层细胞体外培养检测受试药物的毒性作用。结果溪黄草复方对金黄色葡萄球菌的mic值为128？

The experiment used Four kinds of farmland common herbicides (Fenoxaprop、Clethodim、Glyphosate、Fenoxaprop-p-ethl),MDA content、Leaf cell menbrane permeability、Chlorophyll content were tested under three treaments of different medicaments and combined with the growth index.The results showed:(1) When they were used in normal concentration,growth and the others physiological indexes had obviously different under four kinds of herbicides treatments.According to effects of four herbicides on Zanthoxylum bungeanum Maxim ,the effect of Glyphosate randed first,the rest ones were Clethodim、Fenoxaprop-p-ethl and Fenoxaprop.

以农田中常用的6.9%骠马水乳剂、24%收乐通乳油、88.8%飞达红水溶性粒剂、6.9%威霸水乳剂等四种除草剂为供试对象，通过对各药剂的三种浓度处理下花椒叶片中丙二醛含量、叶片中叶绿素含量、叶片中细胞膜通透性的测定，再结合生长量指标进行分析研究，结果表明：(1)在常用浓度下，四种除草剂对花椒生长量及各生理指标的影响有很大差异，飞达红影响最大，依次为收乐通、威霸、骠马。

The ethanol concentration in muscles, blood and vitreous body fluid of the rabbit which was drunk the ethanol and then killed after 2, 3 and 4 hours were respectively measured using head space gas chromatography. At the same time, the ethanol concentrations in muscles of the rabbit killed from 1 to 7 days were measured using the same method.

采用顶空气相色谱法分别测定了家兔灌服乙醇2、3、4小时后被处死后的肌肉、血液和玻璃体液的乙醇浓度，以及家兔被处死后放置1-7天的肌肉乙醇浓度。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！