浓度测定

Results showed that urokinase at 20U/ml was the optimal concentration used. Fibrinolysis kinetics and plasma plasminogen concentration of 27 normal persons and 27 patients with acute myeloblastic leukemia were detected, a significant difference was observed between the two groups. Repetition test showed that the coefficience of variation was 5.24±1.51%. This method could also be used to detect the concentration of plasma fibrinogen.

结果 初步建立了正常人纤溶动力学分析参数，确定了20U/ml尿激酶为最适试验浓度，检测22例正常人，27例急性髓细胞白血病患者纤溶动力学及纤溶酶原含量，显示患病组较正常组差异有显著意义(P.05， P.01)，重复性试验显示该方法的变异系数为(5.42±1.51)%，并可同步测定血浆纤维蛋白原浓度。

Serum and urinary creatinine, urine albumin excretion and NAG were determined photometrically. Urine γ-GT was assayed spectrophotometrically, and urinary B2M was obtained by radioimmunoassay.

每位研究对象均收集24小时尿液样本，测定血清及尿液中肌酐酸浓度、尿中白蛋白排除量、以及尿中 NAG、B2M、γ-GT的活性（以对肌酐酸浓度的比值表示）。

The accuracy and consistency of the new Nessler tube method were systematically studied versus the traditional acid-base titration and average chainlength methods through concentration measurements on polyphosphoric acid with concentrations ranging from 110% to 119%.

通过精确测定浓度范围在110%-119%的多聚磷酸的浓度，配之以理论分析，系统地比较研究了新型Nessler管粘度法和传统的酸碱滴定法、平均链长法的测试精度及稳定性。

If the base was added again to above solution until pH≥13, the resinoid substance was separated from it.

以分光光度法，于740 nm波长下测定含0.98％正丁醇的0.5％PVA与铜盐组成的(pH=10)不同浓度(5～50 mg CuCl_2·2H_2O/100 ml)的水溶液吸光度，并对CuCl_2·2H_2O摩尔浓度作图，它遵守朗伯—比耳定律。

Second, the light adsorption values of bursin with different concentrations at different wavelengths were observed by using ultraviolet spectropolarimeter.

另一种是采用紫外分光光度法测定不同波长下不同浓度囊素的吸光值，建立波长23Onm时囊素浓度与光吸收值之间的回归方程。

The two-photon absorption cross-sections of these styryl derivatives were determined by Z-scan technique, and the benzothiazolium derivatives exhibited the largest TPA cross-sections (10〓s·cm〓/photon) and the best power limiting ability at high operable incident intensity. The results show that the benzothiazolium derivatives are promising organic compounds as two-photon absorption optical limiting materials. 2. Several organic dyes active to 800nm femtosecond laser with D-π-A structure were designed and synthesized.

研究了它们的上转换荧光、上转换激射和光限幅行为，通过改变染料分子共轭体系的大小，首次实现了在576nm-635nm范围内的双光子上转换激光波长的调节，其中噻唑啉碘盐类二苯乙烯化合物的双光子上转换激射峰（576nm）是迄今在1064nm皮秒激光作用下能够得到的最短波长的激光；阴离子显著改变上转换激射效率，但对上转换荧光峰和激射峰的位置影响很小；增加溶液的浓度，上转换荧光光谱和激射光谱红移，并在一定的浓度范围内，能够有效的提高上转换激射效率；利用Z-扫描技术测定了染料分子的双光子吸收截面，发现苯并噻唑类染料分子的双光子吸收截面高达10〓s·cm〓／photon，而且在较大的入射光强范围内，这类染料分子具有较大的光限幅能力，是一类作为双光子吸收光限幅材料很有潜力的染料分子。

The properties of microcapsules,including the surface morphology,the size and distribution were characterized using optical photographic microscopy.Surface tension of the surfactants and interfacial tension of oil /water for sodium dodecylbenzene sulphonate solutions at different concentrations were determined by ring method.

采用光学显微镜表征微胶囊粒径大小及分布、表面形貌等性质，用圆环法测定表面活性剂溶液的表面张力和不同十二烷基苯磺酸钠浓度水溶液/环氧树脂的界面张力，探讨了表面活性剂种类和浓度对MUF环氧树脂微胶囊粒径和形成过程的影响。

In order to study the mechanism of the inhibitory effect of salvia miltiorrhiza and tetramethyl pyrazine on scar fibroblast, the DNA content of fibroblast and the all distribution in cellular cycle was measured by FCM. The hypertrophic scar tissue of chest was chosen for primary culture of fibroblast.

摘 要 应用流式细胞光度分析法测定丹参和川芎嗪对体外培养的瘢痕成纤维细胞DNA相对含量和细胞周期时相变化，发现药物在一定浓度作用下，DNA指数无明显变化；丹参使C2-M期细胞分布增多，G2-M期延长；川芎嗪使G2-M期细胞分布增多，G2-M期、S期延长；药物使细胞群体倍增时间延长，与浓度呈明显直线正相关。

Methods The tetramine samples in 0.5 μg/mL concentration were stored in the environment temperature at 45 ℃, 25 ℃ and 4 ℃. The tetramine concentration were measured by GC-MS in 1, 3, 12, 18 and 39 days, respectively. And then the results of measurement were analyzed by SPSS software.

配制毒鼠强质量浓度为0.5 μg/mL的血液样品，分别置于45 ℃、25 ℃和4 ℃环境中保存，在配制当天及存放3、12、18和39 d用气相色谱－质谱联用法测定其质量浓度，运用SPSS统计软件对结果进行统计分析。

Reactive oxygen species causing DNA oxidative damage comes from two kinds of ways:one is from cellular normal physiological metabolism;the other is from outer environment.Redox-sensitive green fluorescent protein was expressed in Saccharomyces cerevisiae.Recombinant cells were evaluated in monitoring the changes in the redox state of living cells when challenged with toxicologically relevant metal ions NaAsO_2 or Pb(NO_3)_2 by measuring emission intensity at 510 nm with a Hitachi F6500 fluorescence spectrophotometer,roGFP expressed in yeast responded not only to typical membrane-permeant oxidants H_2O_2 and reductants DTT,but also to toxicological metal ion-induced intracellular redox changes in a dose-dependent manner.Moreover,exposure of yeast cells to NaAsO_2 or Pb(NO_3)_2 at concentrations that induced redox changes reported by roGFP caused up to 2～3 fold increases in DNA mutation frequency.This mutagenic effect was largely caused by oxidative stress since blocking the production of hydryl radicals with thiourea significantly reduced the mutation rate as well as delayed the cell death.

本文将对氧化还原状态变化敏感的绿色荧光蛋白roGFP1-R12，在酵母细胞中实现了多拷贝强表达；荧光扫描经强氧化剂H_2O_2和还原剂DTT以及环境中重金属NaAsO_2或Pb(NO_3)_2处理后的酵母细胞悬液，测定510 nm处的荧光发射强度结果显示，表达的绿色荧光蛋白对氧化还原水平敏感，且在510 nm处的荧光强度与一定的重金属浓度呈正相关，即roGFP1-R12在510nm处的荧光发射值随重金属浓度的增高而增强，从而说明重金属对细胞的毒性在一定程度上很可能是通过破坏细胞内的氧化还原平衡发生作用；同时通过该绿色荧光蛋白对胞内氧化还原状态变化的响应情况可以来实时检测环境中的重金属；遗传学的点突变频率及致死率实验数据表明，重金属能导致菌体的点突变频率和致死率升高，且活性氧的清除剂巯基脲能明显降低这种点突变和致死率，说明由重金属引发的这种点突变和致死效应在很大程度上是依赖于重金属对细胞诱导产生的氧化胁迫。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！