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To reseach the mechanisms of ED wtih DM from the acpects of endocrinology, morphology and molecular biology, using rats from NDM and STZ groups as control, the concentration of testosterone, LH and FSH in sera, the microstructure of testicular tissue, bulbocavernous and ischiocavernous muscle tissue, and penile cavernous tissue, the ultrastructure of penile cavernous tissue, and the expression of nNOS mRNA, the content of nNOS and eNOS, the activity of NOS and the concentration of cGMP in the pathway of LARG-NO-cGMP in penile cavernous tissue from ED rats with DM were reseached or examinated in Group A, B and C.

本实验利用第一部分所建立的DM性ED模型,采用NDM及STZ大鼠作对照,通过对A组、B组及C组DM性ED大鼠血清中睾酮、LH及FSH浓度的测定,睾丸组织形态结构的研究从内分泌方面探讨DM性ED的发病机理;通过对阴茎海绵体组织显微及超微结构和球海绵体肌及坐骨耻骨肌显微结构的研究,首次从形态学方面探讨DM性ED的发病机理;通过对阴茎海绵体组织L-ARG-NO-cGMP通路中nNOS mRNA表达、nNOS及eNOS含量、NOS活性及cGMP浓度的检测,从分子生物学方面探讨DM性ED的发病机理。

Methods: Intestine of rats was cannulated for in situ perfusion.

采用大鼠在体肠段灌流实验,以HPLC法测定辛伐他汀和酚红在肠回流液中的浓度,观察吸收部位、药物浓度、pH值3个因素对辛伐他汀肠吸收特性的影响。

MTT assay was adopted to determine the inhibition rate for selecting the best effect time and concentration of Crocin.

采用MTT法测定不同浓度及不同时间藏花素对T24细胞抑制率,选出最佳药物浓度和最佳作用时间。

Within the range of 200mg/L—1000mg/L, the more the initial concentrations of ABA, the more the decolorize velocity of it. The functional relationships between specific growth rate of N1 and temperature, degradation rate of ABA and temperature were constructed, respectively.

通过测定菌株的生长曲线和降解曲线,表明N1菌株的菌体生长和溴氨酸的降解过程是同步进行的;N1菌株对溴氨酸的脱色作用是酶促降解反应的结果,脱色酶需经溴氨酸诱导产生;在一定浓度范围内(200mg/L—1000mg/L),随着溴氨酸浓度的增加,其脱色速度逐渐变慢。

However, this method is discommodious and can not get the instantaneous transformation of dust concentration.

摘 要:环境粉尘浓度的测定一般使用滤膜称重方法测得,该方法费时费力,且不能得到瞬时粉尘浓度的变化。

However,this method is discommodious and can not get the instantaneous transformation of dust concentration.

环境粉尘浓度的测定一般使用滤膜称重方法测得,该方法费时费力,且不能得到瞬时粉尘浓度的变化。

To establish a MPLC method for the content determination of resveratrol in Polygonum cuspidatum Sieb. Et. Zucc, Analytical column SOURCE 5RPC ST 4.6/150 was used with a mixture of acetonitrile+water (30+70,volume ratio ) as mobile phase ,with a flow rate of 1ml/min and the UV detection wavelength was 306nm, The linear range was 0.6-1.6mg/ml (r=0.9919). The average recovery of prodatin was 103.0%-90.4%(RSD=7.21%). The method is convenient and accurate. Result indicated a reverse phase chromatography media based o n polystyrene/divinyl benzene has excellent resolution for resveratrol in cuspidatum. P rovide theoretical support for process development.

色谱条件采用SOURCE 5RPC ST 4.6/150预装柱,以乙腈/水(体积比30/70)溶液为流动相,流速1ml/min、检测波长为308nm,结果表明,白藜芦醇在0.6~1.6mg/ml的浓度范围内峰面积积分值与浓度的相关系数r=0.9919;所测定的虎杖饮片中白藜芦醇平均含量为0.304%,平均回收率为103.0%~90.4%;实验表明,以聚苯乙烯为基架的反相介质对于虎杖中自藜芦醇具有良好的分辨率,适合其有效成分的检测、分离,对虎杖中白藜芦醇制备的科学研究过渡到工艺开拓有很好的借鉴。

Insulin sensitivity, total concentration of Ca in erythorcytes (〔Ca2+〕t), the concentration of cyclic adenosine monophosphate and the activity of the ion pump in the erythrocytic membrance were measured in 31 patients with CHD and 14 normal subjects.

测定CHD组(31例)和正常对照组(14例)的胰岛素敏感性指数、红细胞膜离子泵活性、红细胞总钙浓度和环磷酸腺苷浓度。

Methods A randomized cluster sampling was performed to obtain a representative sample of population in Xinjiang Hefeng pastoral area.Blood wag taken from 632 individuals aged 30 and older for measurements of total cholesterol,triglyceride,hish density lipoprotein cholesterolby automatic biochemieal analyzer.The serum levels of low density lipoprotein cholesterolwas calculated according to the formula.

采用整群随机抽样的方法选取新疆和丰县牧区年龄≥30岁的牧民632人为调查对象,其中哈萨克族325人,蒙古族307人;抽取空腹12 h静脉m 3ml,采用日立7600全自动生化分析仪测定血浆总胆固醇、甘油三酯、高密度脂蛋白胆固醇的浓度,并根据公式计算出LDL-C的浓度,对资料进行汇总,并采用t检验、方差分析或协方差分析的方法观察两民族间血浆LDL-C水平的差异,并进一步采用多元逐步同归分析的统计方法研究其影响因素。

Methods The activity of b5R and the levels of H2O2 in thyroid were measured with potassium ferricyanide as substrate and with the homovanillic acid fluorescence assay,the effect of change of b5R activity on the levels of H2O2 and formation of protein-bound iodine were observed by the addition of b5R inhibitor.

应用荧光分析等技术测定甲状腺b5R活性和H2O2浓度,用b5R抑制剂观察b5R活性变化对H2O2浓度和蛋白结合碘形成影响。

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