浓度测定

The results showed that both the break point on the Ia-c curve and the minimum of the derivative variation corresponding to the λa-c curve agreed very well with the critical micelle concentration of the surfactant in aqueous solution as measured by surface tension technique.

以对二甲氨基苯甲腈为探针，测定它在表面活性剂(C12TABr、SDS、C12E23、C12-3-12·2Br)水溶液中的第二重荧光强度和对应的特征波长对表面活性剂浓度曲线，由Ia-c 曲线的转折点或λa-c曲线对应的一阶导数极小点可以获得临界胶团浓度。

METHODS By the means of viscometry , the effects of reaction time , chitosan and acetic acid concentration on the hydrolytic velocity of chitosan by ultrasonic wave were investigated .

采用粘度测定法，探讨了超声波辐射时间与壳聚糖粘度的关系以及壳聚糖的浓度，醋酸的浓度对降解速度的影响。

Methods Thirty-two New Zeeland rabbits were randomly assigned into two groups as experiment group (n=24) end control group (n=8). Each rabbit in experiment group was given injection with 8.0 mg/kg of hydrocortisone acetate for two times per week, while rabbits in control group were given normal saline in same quantity. Serum glucocorticoid concentration, total cholesterol and triglyceride were determined respectively before and 2, 4, 6 and 8 weeks after treatment. Rabbits were sacrificed at the 2nd, 4th, 6th and 8th week. The rabbits, sacrificed at the same time point, were stratified into group A and group B according to the GC concentration, in an attempt to observe the rate of empty bone lacuna and glucocorticoid receptor expression in the soft tissue around hips.

32只新西兰大白兔随机分成两组：实验组(n=24)每周2次肌注醋酸氢化可的松注射液8.0mg/kg造模，对照组(n=8)每周2次肌注等量生理盐水各组于处理的0、2、4、6、8周抽血测定空腹血清GC血药浓度及而清总胆固醇、甘油三脂水平，并于第2、4、6、8周分批处死动物，将同批处死的实验组动物依据血清GC浓度分为A、B两组，观察股骨头的空骨陷窝率及髋周软组织中糖皮质激素受体的表达。

The toxicity test showed that the aconitic steep had high effects of contact toxicity and lethal toxicity against Calospilos suspecta, with a contact toxicity LC50 of 6.1235mg/L and lethal toxicity LC50 of 22.4905mg/L, and without antifeedant activity and fumigant poison.

毒力测定结果表明，附子浸渍液对五龄期卫矛尺蠖具有较强的触杀作用和毒杀作用，触杀中浓度LC50为6.1235mg/L，毒杀中浓度LC50为22.4905mg/L，无拒食作用和熏蒸作用。

Using EDTA as titratant,murexide as indicator,sodium tartrate ammonium fluoride and sodium thiosulfate as masking agent, nickle concentration was determined in ammonic medium.

以EDTA为滴定剂，紫脲酸铵为指示剂，NH4F、酒石酸钾钠、Na2S2O3为掩蔽剂，在氨性介质中测定镍的浓度；在pH值为8.0的氨-氯化铵缓冲液中，采用滴定比色法测得钴的浓度。

The authers fetched the embryo calvarial peristeum tissue, got human osteoblast by enzyme-assimilating methods and tissue-block culture methods. We observed the morphological change, growth feature and osteogentic capability, of osteoblast during culture in vitro with phase contrast invert microscope, drew the growth curre and identified the cells by alkaline phosphatase dye. At same time, the morphology and bioactivity of 3-5th-generation osbeoblast and anabiotic cells was studied comparatively. 2. titanium particles were examined by scanning electron and the size was determined by semi-automated image analysis. The 3-5 th gereration of human osteoblast were cultured in medium with different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml). Cell growth and proliferation was detected by MTT method after 2、4、6 days that particles were added into medium and ALP activity was measured by kit after 4、7、10 days respectively. 3. With above same methods,the 3-5th generation of human osteoblasts were cultured for 3、6、9days after different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml) were added into the medium and OPG gene expression was quantified by RT-PCR.

1、取人胚胎颅骨骨膜，采有用酶消化法和组织培养法获取成骨细胞体外培养并传代，观察细胞形态，生物特点及成骨特性，并绘制生长曲线同时碱性磷酸酶染色鉴定成骨细胞以及比较冻存前3-5 代与冻存后成骨细胞的特点。2、电镜下观察钛合金颗粒的形态并测量其粒径，将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞共同培养，分别于第2、4、6 天用MTT 法测量细胞增殖情况及4、7、10天用试剂盒检测碱性磷酸酶活性。3、分别将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞基因培养3、6、9 天用RT-PCR 方法半定量测定骨保护素基因mRNA 的表达。

This study was aimed to evaluate the effect of benzyl alcohol on trehaloseloading red blood cells. The RBCs were incubated in 10, 30, 50 and 100 mmol/L concentrations of benzyl alcoholtrehaloe solution at 4℃ for 24 hours. The hemolysis rate of loaded RBCs was detected by using cyanohemoglobin kit, the intracellular trehalose level were assayed by sulfate anthrone method.

为研究苯甲醇对海藻糖负载红细胞的影响，在4℃条件下将红细胞孵育在浓度分别为10、30、50、100 mmol/L的苯甲醇海藻糖溶液中24小时，用氰化血红蛋白试剂盒测定海藻糖负载红细胞的溶血率，用硫酸蒽酮法检测红细胞内海藻糖浓度水平。

Dust and aspiratory dust were monitored personally on the full-mechanized mining work face, full-mechanized tunneling work face and gun tunneling work face in the coal mine to investigate the harm of aspiratory dust to the workers underground.

针对呼吸性粉尘对矿井工人身体健康的危害，对煤矿综采工作面、综掘工作面、炮掘工作面等进行分工种个体性的全尘和呼尘监测，测定了各工作面粉尘的浓度、粉尘分散度和SiO2浓度。

The determination of MD1 concentration in aqueous solution by using bromthymol blue indicator method is described and a linear relationship is observed between absorbance and solution concentration ranged in 10-300 mg/L.

介绍了溴百里酚蓝为指示剂测定水溶液中MD 1质量浓度的方法，在10～300mg/L范围内溶液吸光度与浓度之间有良好的线性关系。

Results showed:(1) The airway responsiveness of smoke control group was higher than that of normal control group, the airway responsiveness of smoke + exercise group was lower than that of smoke control group;(2) Plasma level of cortisol determined immediately after exercise was higher than that determined before exercise, no significant difference occurs between that determined next day morning after all exercise finished in contrast to that determined before exercise;(3) HE staining showed, there was severe chronic pulmonary inflammatory response in smoke control group, which was reduced in the smoke + exercise group;(4) chronic cigarette smoke downregulated the protein and mRNA expression of BKca in smooth muscles of bronchi and bronchioli, exercise increased the mRNA expression of BKca;(5) chronic cigarette smoke downregulated the protein and mRNA expression of Kv1. 5 in smooth muscles of bronchi and bronchioli, exercise increased both the protein and mRNA expression of Kv1. 5 in contrast to smoke control group in bronchioli and did not effect the expression of Kv1. 5 in bronchi;(6) chronic cigarette smoke induced upregulation of FIZZ1/RELMα in bronchial smooth muscle cells, exercise decreased these effect.

结果：(1)吸烟对照组的气道反应性明显增高，吸烟加运动应激可明显降低大鼠的气道反应性；(2)吸烟运动组运动后血浆皮质醇浓度明显高于运动前，但全部运动结束后次日晨测定的血浆皮质醇浓度与运动前无明显差异；(3)HE染色显示，吸烟对照组肺组织出现明显的慢性炎症反应，吸烟加运动应激组比吸烟对照组炎症反应轻；(4)慢性吸烟可降低大鼠大气道和小气道BKca mRNA和蛋白表达，而吸烟加运动应激可使BKca mRNA表达上调，但对小气道的蛋白表达无影响；(5)慢性吸烟可降低大鼠大气道和小气道Kv1.5蛋白和mRNA表达，在小气道吸烟加运动应激可减轻这种作用，大气道则无作用；(6)吸烟使支气管平滑肌的FIZZ1/RELMα蛋白表达明显增强，而吸烟加运动组的RELMα蛋白及mRNA均明显减弱。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。