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And Cucumis sativus L. were tested by bioassay-guided separation. The extracts from P. tomentosa flower with ethanol were extracted with petroleum ether, ethyl acetate and n-butanol. The inhibition rate of the ethyl acetate extract to growth of the 3 kinds of plant radicel were 99.89%, 63.53% and 32.74% respectively at 0.5 mg/mL concentration, and that of petroleum ether were 76.30%, 56.17% and 23.36%.

绒叶泡桐Paulownia tomentosa花乙醇提取物依次用石油醚、乙酸乙酯、正丁醇和水萃取后进行活性测定,结果表明,石油醚萃取物在浓度为0.5mg/mL时,对反枝苋、生菜和黄瓜种子胚根生长的抑制率分别为76.30%、56.17%和23.36%;乙酸乙酯萃取物在浓度为0.5mg/mL时,对反枝苋、生菜和黄瓜种子胚根生长的抑制率分别为99.89%、63.53%和32.74%。

The measured data were fitted with the modified rectangular hyperbola models of the photosynthetic responses to flag leaf intercellular CO2 concentration A/C(subscript i and air CO2 concentration A/C(subscript a aimed to approach the reasonability of the fitted results obtained from the models.

结果表明:由A/C和A/C修正模型所给的光合能力和CO2补偿点几乎相同,但得到的光呼吸速率和初始羧化效率则完全不等;产生这种差异的原因是利用叶片气体交换测定的数据所计算的胞间CO2浓度并不是植物叶片本身的胞间CO2浓度;A/C修正模型比A/C修正模型更为合理是因为A/C所估算的光呼吸速率接近测量值。

The concentration of nitrate was determined indirectly through the effect of nitrate revivification enzyme on nitrate in the existence of NADPH.

在NADPH存在的条件下,通过硝酸盐还原酶将硝酸盐还原为亚硝酸盐,间接测定蔬菜中硝酸盐质量浓度,为检测蔬菜中硝酸盐质量浓度提供一种新方法。

Excellent lichun tomato was selected as transgnic acceptor. Through Kan allergy mensurate of cotyledon callus to establish filtration concentration is 50mg/l, filtration concentration of rootage is 40mg/L.

通过叶片愈伤诱导的卡那霉素敏感性测定,确立了叶片愈伤诱导的卡那霉素的筛选浓度为50mg/L,生根的卡那霉素筛选浓度为40mg/L。

High concentration of SO〓 did not interfere the determination of NO〓 because SO〓 was eluted later than NO〓. The effects of the concentration of sodium borate and CTAB on separation were also examined.

由于加入甲醇后,SO〓在NO〓后出峰,有利于河水中高浓度SO〓存在下,少量NO〓的测定,同时也考察了硼砂和CTAB的浓度对分离的影响。

The factors relevant to the running buffer and their effects on separation were investigated. The buffer solution composed of 30 mmol/L sodium borate and 2.4 mmol/L β-CD was found to be the most suitable electrolyte for separation. The second order dissociation constant range of honokiol was estimated. The content range of honokiol in Cortex Magnoliae Officinalis and was 3.42-5.98 mg/g, while the mean content of honokiol in its preparation 0.021 mg/mL.

优化了厚朴提取物的分离条件,考察了缓冲溶液浓度、pH 值、β-CD的添加浓度对分离的影响,确定了最佳的分离缓冲溶液组成为30 mmol/L 硼砂和2.4 mmol/L β-CD,pH 值为9.48;推测出和厚朴酚的二级离解常数pKa2 的范围为9.94-10.83;在优化的分离条件下,测定了厚朴药材和藿香正气口服液中和厚朴酚的含量分别为3.42-5.98 mg/g、0.021 mg/mL,和厚朴酚的线性范围是0.020-0.405 mg/mL,其线性相关系数R=0.998(n=5),加样回收率为98.4-103.5%,RSD 为3.2-5.7%(n=3)。

Calibration models were established by the spectral measurement of a set of hydrolyzed standard sugar solutions by treating with concentrated sulfuric acid and a chemometrical software.

其原理是基于样品中的糖经浓硫酸处理后生成有紫外吸收的糠醛和羟甲基糠醛,进行紫外波长吸收测定;通过化学计量学软件,根据一组已知糖浓度标准样品的多波长光谱和所对应组成浓度之间的数学关系,建立校正模型。

Methods Firstly,separated splenetic lymphocytes of BALB/c mice;after cultivation of 72 hours with concanavalin Aand several concentration of(1μM,5μM,10μM)ODN_(A151)or ODN_(1612)(10μM),then detected IFNγand IL-4 in supernatant by ELISA.

方法分离小鼠睥淋巴细胞,根据分组加入伴刀豆蛋白A以及不同浓度的ODN_(A151)或对照的ODN_(1612),培养72 h后采用夹心法ELISA测定上清中的IFN_γ和IL-4浓度。

The concentration was determined by LC/MS/MS and a concentration-time profiles could be obtained. The values of AUC were calculated with trapezoid method, t1/2 was calculated according to the last four points in the elimination phase.

采用液相色谱-质谱-质谱联用法,分别测定了给药后不同时刻比格犬血浆中氨苄西林钠和氨苄西林钠/丙磺舒钠浓度,绘制了血药浓度-时间曲线。

METHODS The effects of Honeysuckle and its monomer organic acid against platelet aggregation induced by ADP was determined by turbidimetry in a series of concentration,and the IC_50 was calculated by Logit method.

应用比浊法测定金银花及其单体有机酸类化合物的体外抗ADP诱导的血小板聚集作用,通过系列浓度,用Logit法计算50%聚集抑制浓度(IC50)。

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