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This study was designed to investigate the mechanisms of histone deacetylase inhibitors(sulfonamide anilide compound referred as the "Compound") with antitumor activity against human bladder cancer cells. MATERIALS AND METHODS: Treated with different concentrations of the Compound and HCPT for different periods of time, human bladder cancer cell line BIU-87 growthwas measured by MTT assay.

材料与方法:以不同浓度的Compound和羟基喜树碱作用于体外培养的膀胱癌BIU-87细胞株;采用MTT法检测细胞生长抑制率;以流式细胞术测定不同浓度的Compound和HCPT作用前后膀胱癌细胞的调亡情况及细胞周期的变化;应用RT-PCR分析Compound和HCPT作用前后膀胱癌细胞中P21waf/cif1 mRNA表达的变化。

This study was designed to investigate the mechanisms of histone deacetylase inhibitors (sulfonamide anilide compound referred as the Compound') with antitumor activity against human bladder cancer cells. MATERIALS AND METHODS: Treated with different concentrations of the Compound and HCPT for different periods of time, human bladder cancer cell line BIU-87 growthwas measured by MTT assay.

材料与方法:以不同浓度的Compound和羟基喜树碱作用于体外培养的膀胱癌BIU-87细胞株;采用MTT法检测细胞生长抑制率;以流式细胞术测定不同浓度的Compound和HCPT作用前后膀胱癌细胞的调亡情况及细胞周期的变化;应用RT-PCR分析Compound和HCPT作用前后膀胱癌细胞中P21(上标 waf/cif1)mRNA表达的变化。

Methods: Separated 120 cases with glomerular diseases into chronic glomerular nephritis group (70 cases, including 33 cases with normal serum creatinine, 17 cases with azotemia, 20 cases with uremia, 33 cases accompanied with hypertension), acute nephritis group (12 cases), nephritic syndrome group (38 cases), and selected 30 healthy cases as control group. Detected concentration of NO in serum with nitrate reductase method, and made comparison among groups.

120例肾小球疾病患者分为慢性肾小球肾炎组(70例,其中血清肌酐正常33例,氮质血症17例,尿毒症20例,伴有高血压33例)、急性肾炎组(12例)、肾病综合征组(38例),同期选择30例健康体检者作为对照组,采用稍酸还原酶法测定肾小球疾病患者及对照组血浆NO浓度,并对各组NO浓度进行比较。

Methods: Minimal inhibitory concentration and minimal bactericidal concentration of mulberry leaves were determined in vitro.

测定桑叶水提物及50%,70%,85%,95%醇提物的最低抑菌浓度和最低杀菌浓度。

The spatiotemporal spectral line intensities of the first three atomic lines in Hydrogen Balmer series (λ=656. 28, 486. 13, 434. 05nm) of RF ion source plasma, are measured with calibrated Optical Multichannel Analyzer . Some plasma parameters, including electron temperature, hydrogen atom density and hydrogen ion density, are calculated and analyzed Using Partial Local Thermodynamic Equilibrium theory and Abel transform.

实验采用绝对定标后的光学多道分析系统测定了离子源等离子体不同时间和空间位置的氢原子巴耳末谱线系中前三条谱线(λ=656.28,486.13,434.05nm)的强度,并采用PLTE的理论和Abel变换方法,计算出了高频离子源等离子体的电子温度、氢原子浓度、氢离子浓度等参数在放电的不同阶段和径向分布情况,并进行了简要分析。

Head space gas chromatographical was the better method which can be used to measure the ethanol concentration in the biological sample.

用顶空气相色谱法测定MAC浓度来推断BAC浓度是可行而有效的方法。

On the basis of the pressure correcting method about the measurement of bubble size distribution a photoelectrical capillary probe technique with a micro-computer on line was successfully used to study in detail the effects of various operating parameters such as gas rate, feed rate, feed concentration, NaCl concentration in solution, gas bubbler and gas bubbling state on bubble and foam bubble size distributions in foam fractionation column.

在已建立的光电毛细探头技术测定气泡尺寸分布的压力校正方法基础上,应用光电毛细探头微机在线测量方法,研究了各种操作参数,如气体流速、进料量、进料浓度、溶液中NaCl浓度、气体分布器以及布气状态等,对泡沫分离塔内气泡和泡沫气泡尺寸与分布的影响。

Influence of the concentration of Cr in the leachate and quantity of Pb(CH3COO)2 of Pb(NO3)2 on the reaction of synthesizing yellow chrome was also studied. Finally, according to the criterion and standard method in GB3184-82, some yellow chrome synthesized in the experiments was sampled and the concentration of PbCrO4 in these samples was determined. The following conclusion can be drawn from these experiments.

根据中华人民共和国国家标准GB3184-82中的规定铅铬黄颜料的技术质量指标和检测方法,测定了铬黄样品中铬酸铅含量的厂迄回【、矾士牢位论父泅仰yW盯皿'S川mS达标情况,结论如下:门)反应中重铬酸盐的浓度并不需要达到前人所提出的150~2509·L'的范围,在一定条件下低于此浓度时对铬黄生成反应并无不利影响。

MethodsAfter oral administration of Wuchai Particles(2 g/kg) for the mice , the serum was collected at different time points .The components in the Wuchai Particles and serum were analyzed by HPLC to monitor the changes of the schizandrin in serum so as to draw the curve of concentration connected with time .

方法小鼠灌胃给予五柴颗粒2 g/kg,在不同的时间点采血样,用高效液相色谱方法分析五柴颗粒和血清样品中五味子甲素成分,测定五味子甲素浓度的经时变化,绘制浓度-时间曲线图。

The components in the Wuchai Particles and serum were analyzed by HPLC to monitor the changes of the schizandrin in serum so as to draw the curve of concentration connected with time . ResultsThe main component in Wuchai Particles was schizandrin ; after oral administration of Wuchai Particles , the schizandrin in serum of mice was detected .

方法小鼠灌胃给予五柴颗粒2 g/kg,在不同的时间点采血样,用高效液相色谱方法分析五柴颗粒和血清样品中五味子甲素成分,测定五味子甲素浓度的经时变化,绘制浓度-时间曲线图。

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