浓度测定

Methods: In accordance with conventional reporter gene analysis reagent preparation method, the main component Luciferase, Luciferin and ATP were serial diluted to find the optimal concentration; Reaction solution was prepared according to the optimal concentration of the main component, then ATP were serial diluted to measure their reporter gene. Then standard curve of ATP were drawed. Tumor cell lines H1299 were serial diluted and their ATP were measured by reaction solution prepared to analyse the minimum cell number.

按照常规报告基因分析试剂配制方法，将其中主要成分Luciferase和Luciferin及ATP浓度进行倍比稀释后找到最适合反应的最佳浓度值；按照最佳浓度配制反应液，将ATP浓度倍比稀释测定报告基因数值并绘制标准曲线，分析检测灵敏度；另外选取肿瘤细胞株H1299，倍比稀释后裂解细胞应用配制好的反应液进行ATP检测，分析最低检测细胞数。

The method on trans fatty acids analysis in food is very important. The effects of methylate sodium, potassium hydroxide, sulfuric acid and boron trifluoride in methanol and their different concentration and dosage on TFA analysis were studied. The chromatographic condition and the influence of time and temperature on the esterifation of TFA using boron trifluoride in methanol were optimized. A method for analyzing TFA were established by GC based on boron trifluoride in methanol and RT-2560(100m×0.25mm×0.2μm) capillary column.

研究了甲醇钠－甲醇、氢氧化钾－甲醇、浓硫酸－甲醇和三氟化硼－甲醇4种甲酯化试剂的浓度、用量及甲酯化时间和温度对反式脂肪酸检测的影响，优化了色谱条件，建立了以三氟化硼－甲醇法甲酯化，RT-2560(100m×0.25mm×0.2μm)毛细管柱为基础的反式脂肪酸气相色谱检测方法，C18：1-9 t， C18：1-11 t， C18：2-9 t， 12 t和C22：1-13 t 4种反式脂肪酸的最小检出浓度在0.774.08 mg/kg之间。4种反式脂肪酸不同添标浓度回收率为98.44%101.23%，重复测定的相对标准差在0.22%1.31%之间，有较好的准确度和重现性。

METHODS: A total of 264 chronic HBVinfected patients were enrolled in our study, and were divided into lowlevel HBsAg group(147 cases) and highlevel HBsAg group(117 cases) based on the HBsAg level or were divided into immune tolerance stage, immune clearance stage and nonactive stage based on the natural history. Realtime PCR and microparticle enzyme immunoassay were used to determine the content of HBV DNA and HBV M in serum samples of highlevel and lowlevel HBsAg patients, respectively, then the quantitative results were compared.

对264例慢性HBV感染者的HBsAg浓度(高浓度HBsAg组、低浓度HBsAg组)及自然史(免疫耐受期、免疫清除期、非活动期)进行分组，采用实时荧光聚合酶链反应和微粒子酶免疫试验对147例低浓度HBsA组和117例高浓度HBsAg组的血清标本进行HBV DNA和HBV M的定量测定并进行比较。

When the sulfur concentration is in the range of 0～4 mg/L, the elemental sulfur has a linear relation with the peak current of polarography.

该方法灵敏度高，当元素硫浓度为0.1 mg/L 时仍可以看到明显峰电流；精密度和准确度较好，对1 mg/L 元素硫的测定，标准偏差为0.02；元素硫浓度在0～4 mg/L 浓度范围与极谱峰电流呈线性关系。

S extract had an inhibition effect against Sphaerotheca fuligenea,The inhibition rate was 100% when extract concentration was 20mg/mL and the inhibition rate got to 67.66% even at the concentration of 2.5mg/mL. The extract of E. humifusa had different preventive effects against four strains of S. fuliginea from four districts in Shanghai. The preventive effects on the Qibao and Baoshan were better than those of Nanhui and Qingpu. The microorganism inhibition experiment by filter papers indicated that the extract of E. humifusa had higher inhibition effect against bacteria than on pathogenic fungus. Diameters of the zone of inhibition of salmonellas, Echerichia coli and Staphylococcus aureus were 15.77mm, 12.27mm and 10.53mm respectively. The extract of E. humifusa had a certain effect against Colletotrichum lagenarium, Botrytis cinerea, Rhizoctomia solan and Pyricularia oryza but no effect against Helminthosporium maydis and Gibberella zea. The 4 cation exchange resins and ethanol deposition were used to isolate the active fractions from supernate after alkali deposition.

提取液对黄瓜白粉病菌体外分生孢子萌发抑制实验表明地锦提取液对黄瓜白粉病菌分生孢子的萌发有很好的抑制作用，抑制率随着地锦提取液浓度的增大而增高，当浓度为20mg/mL时，抑制率达到100%，即使在浓度为2.5mg/mL时，抑制率也达到了67.66%；室内盆栽苗期预防性实验表明了地锦提取液对上海4个不同地区的黄瓜白粉病菌均具有不同程度的抑制效果，对七宝和宝山地区的菌株抑制效果优于南汇和青浦地区的菌株；用滤纸片法测定了地锦提取液的抑菌谱，实验结果表明地锦提取液对供试的4种植物病原真菌：黄瓜炭疽病菌、黄瓜灰霉病菌、水稻纹枯病菌和水稻稻瘟病菌均具有一定的抑制效果，而对玉米小斑病菌和小麦赤霉病菌没有抑制效果，对供试的3种人类致病细菌沙门氏菌、大肠杆菌和金黄色葡萄球菌均具有很好抑菌效果，抑菌圈直径分别为：15.77mm， 12.27mm和10.53mm。

To make the wapiti sperm capacitated in vitro, we selected and used the method of sperm-up. By making the rate of acrosome reaction as a main index, and the sperm energy, the survial time and the fertilizated rate of wapiti sperm and hamster mature oocytes as auxiliary indexes, it were carried out to compare the effects of wapiti sperm capacitation in vitro under different temperature, different density of pyruvate sodium, caffeine and heparin and different culture medium.

对马鹿精子，使用上浮法诱导其获能，通过对顶体染色测定精子的顶体反应率并作为主要指标，同时以精子活力、精子存活时间和异种受精率为辅助指标，对精子的获能效果进行评价，以比较不同培养温度、丙酮酸钠浓度、咖啡因浓度、肝素浓度以及培养基对马鹿精子体外获能效果的影响。

The experimental results of stepwise ethanol precipitation showed that the yeild represents the increasing trend with increase of the ethanol concentration, their polysaccharides contents have significant changes, and at the ethanol concentration of 30% and 70%, polysaccharides contents are up to maximum. Molecular weights of endopolysaccharides and exopolysaccharides are pockety.

分级醇沉实验表明，各醇沉浓度对应的得率都随醇浓度的升高而呈增加趋势，多糖含量变化起伏较大，醇浓度为30%和70%时所得多糖含量较高，虫草胞内和胞外多糖分子量分布都很不均匀；分步醇沉测定了各部分多糖所占的比例。

The effect of gold nanoparticles on the resolution and selectivity of separation was investigated. The influence of separation voltage, pH and buffer concentration on the separation of aminobenzoic acids was also examined.

探讨了修饰金纳米浓度、修饰时间以及缓冲溶液加入一定浓度的金纳米对三种氨基苯甲酸分离和测定的影响，优化了分离电压、酸度以及缓冲溶液浓度等实验条件。

Methods Sixteen adult Sprague-Dawley rats were enrolled, from which anticoagulative erythrocyte suspension was made, and was incubate with equivalent volume of 1 time,2 times,4 times and 8 times of clinical therapeutic blood concentration of different local anesthetics respectively. Erythrocyte-yeast rossete and Tumor-erythrocyte rosette rate were measured.

选择成年SD大鼠16只，取抗凝血制备成所需浓度的红细胞悬液，与等体积的临床血药浓度、2倍、4倍及8倍临床血药浓度的各研究麻醉药物孵育后，加入补体致敏酵母菌或用自身血浆作为补体来源致敏的肿瘤细胞，测定红细胞、酵母菌花环率和肿瘤细胞-红细胞花环率。

The changes of Cr and Fe concentration were measured during Co-culture experiment and slurry experiments. The microbial reducing ability of different chromate concentrations and the effect of chromate on dissimilatory iron reduction in paddy soil were investigated.

通过接种土壤微生物的混合培养及土壤泥浆培养试验，测定了厌氧培养过程中Cr和Fe浓度的变化，研究了不同浓度铬酸盐的微生物还原能力及不同浓度铬酸盐对水稻土中氧化铁还原的影响。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。