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Aqueous extract of cotton has allelopathic effect on Raphanus sativus, Brassica campestris, Lactuca indica, Brassica campestris, Echinochloa crusgall, Setaria viridis and Abutilon theophrasti and its inhibition effect gradually strengthens with increasing concentration. Aqueous extracts from stems, leaves and roots of cotton inhibit or accelerate seed germination and seedling growth, and activity of enzyme of seedling root of cotton. While aqueous extracts from stems and leaves of cotton are 0.02 gmL^(-1), root length, shoot length, lateral root number and dry weight of cotton respectively increase by 10.3%, 3.1%, 22.9% and 2.4% over the control. The above mentioned indexes are lower than the control by 34.4%, 39.2%, 40.6% and 19.0% respectively as the aqueous extracts are 0.1 gmL^(-1). At the same time, root activity and SOD activity decrease by 38.2% and 36.7%, while POD activity and MDA content increase by 52.3% and 36.5%. Growth indexes of plant receptors increase at low concentrations but decrease at high concentrations of aqueous extracts. The allelopathic effect of aqueous extract of stems and leaves is stronger than that of root.

结果表明:棉花植株水浸提液对萝卜、白菜、莴苣、油菜、稗草、狗尾草、苘麻均具有化感效应,随着作用浓度增加,抑制效应逐渐加强;棉花茎叶和根系水浸提液对棉花种子萌发、幼苗生长、幼苗根系活力、根系酶活性有抑制或促进作用,当茎叶浸提液浓度为0.02 gmL^(-1)时,棉花根长、苗高、侧根数、干重分别比对照增加10.3%、3.1%、22.9%和2.4%,当浓度为0.1 gmL^(-1)时,上述指标与对照相比分别降低34.4%、39.2%、40.6%和19.0%,而根系活力、SOD活性比对照降低38.2%、36.7%,POD活性、MDA含量较对照增加52.3%和36.5%,不同浓度棉花植株水浸提液对棉花生长呈现"低促高抑"的规律,且茎叶浸提液作用效应大于根系浸提液。

The results indicate that the acetophenone conversion is affected orderly by substrate concentration, reaction time, reaction temperature and yeast concentration.

结果表明,影响苯乙酮转化率的因素依次为底物浓度、反应时间、反应温度和酵母浓度,影响1苯基乙醇对映选择性的因素依次为反应温度、底物浓度、酵母浓度和反应时间。

The higher the concentration of Cefotaxime was, the lower the rate of differentiation was; when the concentration of Cefotaxime was high 400~500mgL^(-1, the growth of callus was inhibited. However, the effect of Carbenicillin on callus was little and the differentiation of adventive buds was promoted under appropriate concentration. When the Carbenicillin's concentration was 200mgL^(-1), the differentiation rate reached the maximum 68.8%.

头孢霉素浓度愈大,213品种不定芽分化频率愈低,当头孢霉素浓度较高400~500mgL^(-1时,愈伤组织生长受到抑制;而羧苄青霉素对愈伤组织影响很小,在适宜的浓度下能促进不定芽分化,213品种在羧苄青霉素浓度为200mgL^(-1)时,分化频率达到最大值68.8%。

Analyzing the change of DH, PCL and active calcium, and selecting each of best method. The results:(1) Dealing with the bone under high pressure ①High content of protein in sheep bone or bone soup:0.05MPa, 2h, 1:2;②Low amino acids in bone powder:0.13MPa, 4h, 1:3; Low amino acids in bone soup: 0.10MPa, 3h, 1:4;(2) Hydrolysis the bone soup with enzymes ①High content of calcium: neutral enzyme, 1500u/L, 6h;②High DH: pancreatic enzyme, 1:100, 6h;③ Low PCL: Pancreatic enzyme, 1:125, 6h; High PCL: alkalescency enzyme,

结果表明:A、高压①骨粉及骨汤蛋白质含量最高:压力为0.05MPa,高压2.0h,骨水比为1:2;②骨粉中氨态氮最低:压力0.13MPa,高压4.0h,骨水比为1:3;骨汤中氨态氮最低:0.10MPa,3.0h,骨水比1:4;B、酶解①游离钙含量最高:中性蛋白酶,酶浓度1500u/L,酶解6h;②水解度最高:胰蛋白酶,酶浓度1:100,酶解6h;③PCL值最低:胰蛋白酶,酶浓度1:125,酶解6h;PCL值最高:碱性蛋白酶,酶浓度1500u/L,酶解8h。

The results:(1) Dealing with the bone under high pressure ① High content of protein in sheep bone or bone soup:0.05MPa, 2h, 1:2;② Low amino acids in bone powder:0.13MPa, 4h, 1:3; Low amino acids in bone soup: 0.10MPa, 3h, 1:4;(2) Hydrolysis the bone soup with enzymes ① High content of calcium: neutral enzyme, 1500u/L, 6h;② High DH: pancreatic enzyme, 1:100, 6h;③ Low PCL: Pancreatic enzyme, 1:125, 6h; High PCL: alkalescency enzyme,

结果表明: A 、高压①骨粉及骨汤蛋白质含量最高:压力为0.05MPa ,高压2.0h ,骨水比为1:2;②骨粉中氨态氮最低:压力0.13MPa ,高压4.0h ,骨水比为1:3;骨汤中氨态氮最低:0.10MPa ,3.0h ,骨水比1:4; B 、酶解①游离钙含量最高:中性蛋白酶,酶浓度1500u/L ,酶解6h ;②水解度最高:胰蛋白酶,酶浓度1:100,酶解6h ;③ PCL 值最低:胰蛋白酶,酶浓度1:125,酶解6h ; PCL 值最高:碱性蛋白酶,酶浓度1500u/L ,酶解8h 。

And then HepG2.2.15 cellswere cultured with different non-cellulotoxic concentration ofα-amanitin or 3TC for six days.

采用MTT法检测不同浓度α-鹅膏毒肽对体外培养的HepG2 2.2.15细胞株的毒性,然后在无毒浓度下用不同浓度α-鹅膏毒肽或拉米夫定与HepG2 2.2.15细胞共培养6天,分别在第3天和第6天收集细胞培养上清液,采用时间分辨免疫荧光法测定上清HBsAg、HBeAg浓度;收获细胞提取总RNA,行逆转录聚合酶链反应检测细胞内HBsAg和HBcAg mRNA的表达。

The results were as follows:The first, there were significantly differences percent between 0.6μg/mlgroups and other three groups (0.2μg/ml groups, 0.4μg/ml groups and 0.8μg/mlgroups) in 2 hour groups; and there were significantly differences between0.4μg/ml groups and other three groups in 4 hour groups; and there weresignificantly differences between 0.6μg/ml groups and other three groups in 6hour groups; and there were significantly differences between 0.4μg/ml groupsand other three groups in 8 hour groups on the metaphases (P<0.05), in theexperiment of the effect on the mouse 4- cell embryo stage single blastomere ofcolchicine with different concentrations and duration, by x~2 statistical analysis.

实验结果如下:1、在确定秋水仙素不同处理浓度和时间对小鼠4-细胞期胚胎单卵裂球中期分裂相数影响的实验中,经统计分析,阻断培养2小时组中0.6μg/ml浓度组与其他三组差异显著(0.2μg/ml组、0.4μg/ml组和0.8μg/ml组);阻断培养4小时组中0.4μg/ml浓度组与其他三组差异显著;阻断培养6小时组中0.6μg/ml浓度组与其他三组差异显著;阻断培养8小时组中0.4μg/ml浓度组与其他三组差异显著(P<0.05)。

The results were as follows:①The potassium ion concentration in serum were 4.2,3.7 and 4.4 mmol/L, resp., in sikas, C.aibirotris and red deer examined of Qinghai province.②The potassium ion concentration in whole blood were 28.3, 27.4 and 28.9 mmol/L, resp.③The potassium ion concentration in erythro...

结果发现:①被检青海梅花鹿、白唇鹿和马鹿的血清钾离子浓度分别为4.2、3.7和4.4 mmol/L;②全血钾离子浓度为28.3、27.4和28.9 mmol/L;③红细胞钾离子浓度分别为70.5、75.4和74.4 mmol/L;④按全血钾离子浓度或红细胞钾离子浓度判定,全部被检青海三种鹿的红细胞钾型都是高血钾型。

In the concentration rage used the rete constant k is directly proportional to the concentration of TEMPO while is proportional to the concentration of NaBr in a relatively lower range and tends to level off at higher concentration.

在本研究所用的TEMPO浓度范围内,氧化反应这率与TEMPO的浓度成正比。在较低浓度时,氧化反应速率与NaBr用量也成正比,但浓度较大时,氧化反应这率基本不随NaBr浓度增大而增大。

In the concentration rage used the rete constant k is directly proportional to the concentration of TEMPO while is proportional to the concentration of NaBr in a relatively lower range and tends to level off at higher concentration.

在本研究所用的TEMPO浓度范围內,氧化反应这率与TEMPO的浓度成正比。在较低浓度时,氧化反应速率与NaBr用量也成正比,但浓度较大时,氧化反应这率基本不隨NaBr浓度增大而增大。

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