浓度

Priority PAHs recommended by USEPA were all detected, and the mass concentrations of individual PAHs was between 1.49 and 87.43ngg^(-1), The PAHs with low molecular weight, such as Nap, Flu, Ace were observed in relatively low mass concentrations. The PAHs with high molecular weight, such as Chrysene, Indeno [1, 2, 3-cd] pyrene, Benzo perylene were relatively abundant and the mass congcentration of BghiP was the highest. The total mass congcentration of 16 PAHs exceeded standard of Holand from 10 to 40 times, and in middle pollution level comparatively with other districts.

美国环保总署推荐优先控制的16种多环芳烃均被检出，多环芳烃单体的质量浓度在1.49~87.43ngg^(-1)扩之间；其中萘、药、苊等低分子量芳烃的质量浓度相对较低；窟、茚并[1， 2， 3-cd]苝、苯并苝等高分子量芳烃的质量浓度相对较高，苯并苝的质量浓度最高。16种多环芳烃的质量总浓度超过荷兰政府规定无污染土壤PAHs值的10~40倍；与国内外其他地区相比较，多环芳烃污染处于中等水平。

As the DDP group A549DDP cells were treated with IC10 of cisplatin for 48 hours. As the DDP＋Rh2 group A549DDP cells were treated with IC10 of cisplatin and IC5 of gensenoside Rh2 for 48 hours. As the controlling group A549DDP cells were not treated with any kinds of drugs. Changes in cellular morphology were observed by fluorescence microscopy. Peak value apoptosis of A549DDP cells were inspected by flow cytometry. State of PTP was evaluated by ultraviolate spectrofluorometer. Changs of calcium in cells and membrane potential of mitochondrion were determined by flow cytometry. Release of cyt-c from mitochondrion with westton blot Radiative activity of〓Tc-MIBI ingested by cells was measured withγcounter. Results: IC50 of cisplatine to A549 cells was 24uM.

以无毒浓度的Rh2单独作用于A549DDP细胞作为Rh2组，以低效浓度的顺铂单独作用于A549DDP细胞作为DDP组，以无毒浓度的Rh2和低效浓度的顺铂联合作用于A549DDP细胞作为Rh2+DDP组，作用时间为48小时，以不加药物干预作为对照组，Hoechst33258染色荧光显微镜下观察细胞形态的变化，流式细胞仪检测细胞凋亡峰；紫外光分光光度计检测线粒体PTP开放情况，流式细胞仪检测细胞内钙离子浓度及线粒体跨膜电位的变化，Westton blot检测线粒体内细胞色素C释放情况及Caspase-3的活性，γ计数仪检测细胞摄取〓Tc-MIBI放射性活性。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

Diastatic power also enhanced except that the KCr(SO_4)_2 concentration was 250 mg·kg~(-1). When KCr(SO_4)_2 concentration was 100 mg·kg~(-1), diastatic power and total score achieved highest which were 311.2 W·K and 57.3.(2)Beer yeast grew rapidly in wort when KCr(SO_4)_2 concentration were 25 ~ 200 mg·kg~(-1). Yeast growed weakly when KCr(SO_4)_2 concentration was 250mg·kg~(-1). Cells of

3富铬麦芽制成的麦汁用于发酵，后酵结束时，KCr(SO_4)_2不同处理浓度降低了pH值和外观糖度；KCr(SO_4)_2处理浓度在25～200 mg·kg~(-1)时促进了酵母生长；KCr(SO_4)_2处理浓度达到200 mg·kg~(-1)和250 mg·kg~(-1)时，α-氨基氮的利用率小于对照；KCr(SO_4)_2处理浓度达到200 mg·kg~(-1)和250 mg·kg~(-1)时，双乙酰峰值的出现时间比对照晚且峰值小于对照，其他处理浓度时，双乙酰的峰值出现时间与对照一致且

And then the two different turfgrass seed germination tests conducted, results show that: 0.1 percent concentration of hydrogen peroxide concentration of 0.01% of the "Bi-care" and the concentration of 0.29 percent for the "God LU Hong Lu," The three kinds of reagents soaking treatment can significantly improve the wild Pennisetum and Eragrostis seed germination and growth of 0.10 percent by the concentration of the soaking H2O2 solution, then 0.29% concentration of the "God LU Hong Lu" after soaking solution, wild Pennisetum Eragrostis seeds and seed germination energy, germination percentage, germination index, root length and were significantly higher than control to deal with the seeds, but also to varying degrees higher than the other chemical reagents used to deal with the seeds, rapid germination and tidy.

然后对两种草坪草不同的种子处理进行了发芽试验，结果表明：浓度为0.1%的过氧化氢、浓度为0.01%的&碧护&和浓度为0.29%的&鲁虹神露&这三种试剂浸种处理能显著提高野生狼尾草和大画眉草种子的萌发和生长，经浓度为0.10%的H2O2溶液浸种，再经浓度为0.29%的&鲁虹神露&溶液浸种处理后，野生狼尾草种子和大画眉草种子的发芽势、发芽率、发芽指数、根长和芽长均显著高于空白对照处理的种子，也不同程度的高于用其他化学试剂处理的种子，发芽快而整齐。

Root-derived organic carbon is composed of three compartments: root detritus, root eaudates and root symbionts, which represents a large important and potential C sink in forest ecosystems, The known responses of root derived organic carbon and heterotrophic microbes to elevated CO2 were reviewed. Overall, increases in root biomass, productivity, and exudation indicate an augmentation of C inputs into the soil via roots, although changes in root lifespan are still unclear and may modify this flux positively or negatively, Altogether, changes in root chemistry and morphology that may augment soil C storage under elevated CO2 include decreased N concentration and wider root diameter, Alternately, a shift in rooting depth upwards in the soil profile may increase average turnover rates of root detritus. Effects of CO2 concentration on C qualities of root exudates and root symbionts are the least understood components discussed here, Changes of soil heterotropic microbial activity and community under elevated CO2 still remained large uncertainties.

林木根源有机C包括根东通过根枯落物、根系分泌物和根共生菌周转3条途径向土壤输入的有机C，它是森林生态系统中一个重要的、潜在的C汇，综述了根源有机C与其微生物对CO2浓度升高的响应，虽然对根系寿命的变化尚不清楚，但CO2浓度升高将导致根系生物量、生产量、死亡量和分泌物的增加；同时，CO2升高亦促使根共生菌生物量的增加而增加了共生菌的C归还潜力，表明CO2升高使根源有机C的输入增加了，CO2农度升高情况下，根系化学性质和形态特征的这些变化均有利于增加土壤C的吸存；而根分布深度的降低则对土壤C吸存不利；CO2浓度升高对根分泌物和根共生菌质量的影响研究则极少，CO2浓度升高下土壤微生物活性和群落组成的变化存在较大的不确定性，目前CO2浓度升高下林木根源有机C对森林长期C吸存的贡献仍很不清楚。

The results as fallows:(1) The color became dark with the increase of NaOH~* concentration, Cu~(2+) concentration and loadage, it became light with the increase of silver content, NH_3·H_2O concentration, Ag~+ complexing reagent A concentration, Cu/Ag ration and granularity, and it firstly became light and then became dark with the increase of NaOH concentration.

具体结果如下：(1)镀银铝粉的颜色随NaOH~*浓度、Cu~(2+)浓度、装载量的增加逐渐变深，随含银量、NH_3·H_2O浓度、Ag~+络合剂A浓度、Cu/Ag比、粉体粒度的增加变浅，随NaOH浓度的增加先变浅后变深。

The influence of NaOH~* concentration, Cu~(2+) concentration, NH_3·H_2O concentration, Cu/Ag ration, Ag~+ complexing reagent A concentration, NaOH concentration, loadage, silver content and granularity on the color, compact resistance, apparent density and anti-oxidation property of silver coated aluminum powder and silver plating time were studied by condition experiment.

通过条件实验，我们探讨了NaOH~*浓度、Cu~(2+)浓度、NH_3·H_2O浓度、Cu、Ag比、Ag~+络合剂A浓度、NaOH浓度、装载量、含银量、粉体粒度对镀银铝粉颜色、松装密度、压实电阻、抗氧化性及镀银时间的影响。

In a population of 11,770 NHANES participants from 1999 through 2004 who were 4 to 19 years old, the investigators looked at the relationship of headache with BMI; CRP; homocysteine levels; serum and red blood cell folate levels; vitamin B12; methylmalonic acid levels; total cholesterol, high-density lipoprotein cholesterol, and non-HDL cholesterol levels; triglyceride levels; uric acid levels; and platelet count.

在1999年到2004年之间，共有11，770位NHANES参与者，参与者的年龄介于4~19岁，研究者研究头痛与BMI、CRP、同半胱胺酸浓度；血清与红血球叶酸浓度；维生素B12；甲基丙二酸；总胆固醇、高密度脂蛋白、以及非HDL胆固醇浓度；三酸甘油脂浓度；尿酸浓度与血小板数目。

In a population of 11,770 NHANES participants from 1999 through 2004 who were 4 to 19 years old, the investigators looked at the relationship of headache with BMI; CRP; homocysteine levels; serum and red blood cell folate levels; vitamin B12; methylmalonic acid levels; total cholesterol, high-density lipoprotein cholesterol, and non-HDL cholesterol levels; triglyceride levels; uric acid levels; and platelet count.

在1999年到2004年之间，共有11，770位NHANES参与者，参与者的年龄介於4~19岁，研究者研究头痛与BMI、CRP、同半胱胺酸浓度；血清与红血球叶酸浓度；维生素B12；甲基丙二酸；总胆固醇、高密度脂蛋白、以及非HDL胆固醇浓度；三酸甘油脂浓度；尿酸浓度与血小板数目。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。